Fluorescent Amino Acid Initiated de novo Cyclic Peptides for the Label-Free Assessment of Cell Permeability**

The major obstacle in applying peptides to intracellular targets is their low inherent cell permeability. Standard approaches to attach a fluorophore (e. g. FITC, TAMRA) can change the physicochemical properties of the parent peptide and influence their ability to penetrate and localize in cells. We report a label-free strategy for evaluating the cell permeability of cyclic peptide leads. Fluorescent tryptophan analogues 4-cyanotryptophan (4CNW) and β-(1-azulenyl)-L-alanine (AzAla) were incorporated into in vitro translated macrocyclic peptides by initiator reprogramming. We then demonstrate these efficient blue fluorescent emitters are good tools for monitoring peptide penetration into cells.     

Global climate change: Implications for thailand's energy systems

An energy/environmental model has been developed to estimate Thailand's energy consumption and CO₂ emissions through the end of the National Tenth Plan (year 2011). The projection suggests that fossil-fuel consumption, not deforestation, will be the major source of emissions during 1990–2011. By the year 2011, energy-efficiency improvement measures could bring about a 14% reduction of CO₂ emissions. Any reduction beyond this level would require switches of fuel mix in the power, transportation, and industrial sectors. Fuel shifts in the power sector alone could cut emissions by up to 20% to 30%. In the longer run, Thailand should consider adopting unconventional sources of energy, as well as make use of reforestation policy to absorb future CO₂ emissions.