Reversible or irreversible modification of [3H]PAF binding on rabbit platelet membranes differentiates various PAF receptor antagonists

[³H]Platelet-activating factor (PAF) binding to rabbit platelet membranes was examined before and after 20 min preincubation at 25°C in the presence of PAF, lysoPAF, or of five different PAF receptor antagonists (L 652731, BN 52021, WEB 2086, BN 52111 and BN 52115). When platelet membranes were not washed after preincubation with PAF or PAF antagonists, no significant specific binding of [³H]PAF was observed, which suggests full occupancy of the binding sites. When membranes were extensively washed, full recovery of specific [³H]PAF binding was attained with L 652731 and partial recoveries (60%, 55% and 30%) were reached with BN52021, WEB 2086 and PAF, respectively; no recovery was seen with the dioxolanes BN 52111 and BN 52115. Scatchard analysis of the binding data indicated that no significant change in the dissociation constant (K_d) and maximum number of binding sites (B_max) occurred after preincubation of platelet membrane with L 652731, whereas a reduction of B_max was observed when PAF and BN 52021 were measured. When platelet membranes were preincubated with WEB 2086, B_max and K_d significantly increased. The data suggest differing binding properties for PAF and the PAF antagonists. Some of the PAF antagonists may tightly bind to the PAF receptor site(s) and/or irreversibly modify or downregulate PAF recognition sites. Our results also suggest that the interaction of PAF receptor antagonists with PAF receptor can be divided into at least two components, namely a reversible component and an irreversible one. Based on a paper presented at the Third International Conference on Platelet-Activating Factor and Structurally Related Alkyl Ether Lipids, Tokyo, Japan, May 1989.     

Purification of the human anionic polypeptide fraction of the apo-bile lipoprotein complex by zonal ultracentrifugation

The two main proteic constituents of the human Apo-bile lipoprotein complex (BLC), i.e., the anionic polypeptide fraction (APF) and the IgA fragments, were separated by preparative zonal ultracentrifugation using a sucrose gradient containing 1.5 mM glycodesoxycholate. The purification of the APF was verified by sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis and immunology, and its amino acid composition then was determined. This procedure was used to obtain a polyclonal antiserum directed solely against the APF.     

Improving Neural Networks for Mechanism Kinematic Chain Isomorphism Identification

Detection of isomorphism among kinematic chains is essential in mechanical design, but difficult and computationally expensive. It has been shown that both traditional methods and previously presented neural networks still have a lot to be desired in aspects such as simplifying procedure of identification and adapting automatic computation. Therefore, a new algorithm based on a competitive Hopfield network is developed for automatic computation in the kinematic chain isomorphism problem. The neural approach provides directly interpretable solutions and does not demand tuning of parameters. We have tested the algorithm by solving problems reported in the recent mechanical literature. Simulation results show the effectiveness of the network that rapidly identifies isomorphic kinematic chains.     

Multiwalled Nanotubes: Their Formation by Irradiating Graphite with High Doses of Electrons

We report the production of carbon nanotubes by high dose of electron irradiation. The irradiation was performed with a 2 MeV Van de Graaff accelerator, while the irradiation conditions were the following: voltage 1.3 MeV, current 5 µA, dose rate 25 kGy/min, and total dosage 1000 kGy. The samples were analyzed in a high-resolution transmission electron microscope. The main features observed on the samples, were huge nanotubes of several nanometer long and few nanometer wide, which are capped at one end. It is good to point out, that at this level of irradiation, we were not able to find either onion-like or particle structures throughout the material, as it is usual in similar hexagonal structures. This behavior could be attributed to the level of irradiation used to create the nanotubes under investigation.     

A variable rate multipulse speech coder for CDMA cellular systems

Variable Rate (VR) speech coders are classified into: source-controlled VR coders where the rate is selected depending on the local character of the speech, and network-controlled VR coders where an external control signal selects the coding rate. The first category benefits from the variable rate channels used by Code Division Multiple Access (CDMA) mobile communications. The second category is indispensable for the right behaviour of the CDMA systems under conditions as high traffic levels. The VR speech coder presented in this communication exhibits both types of control. The source control is achieved by means of a Voice Activity Detector (VAD) and a phonetic classifier. The network control acts on the selection procedure of the multipulse excitation sequence to the synthesis filter. This is the main advantage of our VR MultiPulse speech coder because by means of an external signal the bit rate can be changed only every 4 msec, without transitions or distortions. Considering one-way communication, six different operating rates can be externally selected ranging from 4.8 to 9.1 kbps for the active frames; an average bit rate of 380 bps is required for the noise frames.This work has been partly funded by the Spanish Research National Plan under grant no. TIC92-0800-C05-02 and by Northern Telecom.     

Behavior of biliary phospholipids in intestinal lumen during fat digestion in rat

The phospholipids present in the intestinal lumen of rats following ingestion of triglycerides are of biliary origin. They consist of lecithins accompanied by a small proportion of lysolecithins. Their behavior in comparison with the other lipid constituents of the intestinal content was studied by subjecting the latter to gel filtration on an agarose column in the presence of a solution of 6 mM sodium taurocholate in 0.1 M NaCl. Part of the phospholipids is present with the triglycerides and diglycerides in the emulsified phase excluded from the gel where pancreatic lipase and colipase also are found. The remainder is found in optically clear fractions containing fatty acids, monoglycerides, and bile salts. These fractions are eluted at 2.0 column volumes, while mixed fatty acids, monoglycerides, bile salts micelles emerge from the column at 2.4 column volumes in the same chromatographic conditions. This difference in behavior may be explained by the presence of biliary lecithins. This presence could have an important bearing upon the mucosal uptake of the lipolysis products of triglycerides.     

Balanced pasture fertilization in the Basque Country: I. Phosphorus and potassium budgets on dairy farms

Dairy farming is the main agricultural activity of the Basque Country. A dairy farm is characterized as a system with soils and crops, forage, cattle and manure as main components, and in such a system, nutrient cycling is very important to maintain soil fertility and optimize forage production. To quantify nutrient transfers in the cycle, a simple system was developed and has been applied to seventeen farms to examine its ability to achieve a balanced P and K fertilization. These farms have provided data on inputs (fertilizer, feeds, concentrates), pasture and manure management, and outputs (milk production), and soil samples have been taken from farm pastures. Phosphorus and K in excreta and uneaten pasture is used with a relatively high efficiency as suggested by the relatively high efficiency of P and K utilization by the pasture that usually ranges from 70 to 90%. Concentrate feeding (3000 kg cow^–1 yr^–1) represents one of the main P and K inputs in Basque Country dairy farms, averaging 26 and 66 kg ha^–1, respectively. Besides, release of K in the soil through slow liberation from non-exchangeable sites was estimated as 30 kg ha^–1. Thus, a high efficiency in excreta recycling would diminish substantially P and K mineral fertilizer needs. Farm nutrient budgets appear to be a convenient tool for determining nutrient shortages and surpluses at farm level, and thus they are considered as a first step to support a better management of maintenance fertilization of permanent pastures.