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1.
Dr. Adarshi P. Welegedara Dr. Ansis Maleckis Ruchira Bandara Dr. Mithun C. Mahawaththa Iresha Dilhani Herath Yi Jiun Tan Dr. Angeliki Giannoulis Prof. Daniella Goldfarb Prof. Gottfried Otting Prof. Thomas Huber 《Chembiochem : a European journal of chemical biology》2021,22(8):1480-1486
The selenol group of selenocysteine is much more nucleophilic than the thiol group of cysteine. Selenocysteine residues in proteins thus offer reactive points for rapid post-translational modification. Herein, we show that selenoproteins can be expressed in high yield and purity by cell-free protein synthesis by global substitution of cysteine by selenocysteine. Complete alkylation of solvent-exposed selenocysteine residues was achieved in 10 minutes with 4-chloromethylene dipicolinic acid (4Cl-MDPA) under conditions that left cysteine residues unchanged even after overnight incubation. GdIII−GdIII distances measured by double electron–electron resonance (DEER) experiments of maltose binding protein (MBP) containing two selenocysteine residues tagged with 4Cl-MDPA-GdIII were indistinguishable from GdIII−GdIII distances measured of MBP containing cysteine reacted with 4Br-MDPA tags. 相似文献
2.
Geoffrey M. Rowe Jay N. Meegoda Andris Jumikis Mark J. Sharrock Nishantha Bandara Chamil H. Hettiarachchi 《Canadian Metallurgical Quarterly》2004,130(6):924-934
Abstract: This paper describes the research funded by the New Jersey Department of Transportation to develop an automated technology to monitor segregation during construction of hot-mix asphalt concrete pavements. A Laser-based system was used to measure surface texture and to detect segregation. Two segregated test sections and a control test section were tested to evaluate the applicability of Laser texture method to detect and quantify segregation. Laser texture data were gathered from all three sections. Ratios of texture in segregated areas to that in nonsegregated areas were set as the basis for detection of different levels of segregation. By combining the level of segregation and extent of segregation, an AREA index was developed to determine the acceptability of a pavement section. Based on AREA index, pay adjustment factors were proposed to reduce the payment to account for loss of pavement life due to segregation. Further remedial actions were proposed to correct segregated pavement sections with acceptable AREA index. Based on the above concepts, Windows-based computer program NJTxtr was developed to detect and quantify segregation. This computer program uses the Laser-equipment-collected pavement texture data and determines whether the pavement section is acceptable or unacceptable based on the level of segregation within a pavement section, and provides bonus or penalties to the contractor. The paper describes a novel technology using laser and associated software for construction quality control of asphalt concrete pavements. The proposed methodology was applied to detect segregation in an interstate highway section in New Jersey, and this section was repaved based on visual observation and recommendation from this study. 相似文献
3.
Bandara T. M. W. J. Wickramasinghe H. M. N. Wijayaratne K. DeSilva L. Ajith Perera A. A. I. 《Journal of Materials Science: Materials in Electronics》2021,32(22):26758-26769
Journal of Materials Science: Materials in Electronics - Dye-sensitized solar cells (DSCs) have evolved through the highest devotion of the research community as a low-cost alternative for... 相似文献
4.
In this report, we investigated an infrared photon active catalytic system for the conversion of solar energy into chemical energy in which we introduced an effective “Dark Photocatalyst” for photolysis of water. The Ag2O/TiO2 composite catalyst containing 28% of silver and 72% of titanium was synthesised by a simple chemical deposition method. The catalyst was found to be active in the infrared region of the solar spectrum or in the dark and hydrogen production was demonstrated for the first using low-energy 800–1200 nm IR radiation. Sub-band gap phonon assisted multi-photon excitation is proposed for the observed infrared photocatalytic activity of Ag2O/TiO2 composite. 相似文献
5.
A.K. Arof M.F. Aziz M.M. Noor M.A. Careem L.R.A.K. Bandara C.A. Thotawatthage W.N.S. Rupasinghe M.A.K.L. Dissanayake 《International Journal of Hydrogen Energy》2014
This paper reports the effect of using a mixed iodide salt system with two dissimilar cations to enhance the efficiency of dye-sensitized solar cells made with polyvinylidenefluoride (PVdF) based gel electrolyte. Instead of a single iodide salt, a mixture of potassium iodide (KI) with a small K+ cation and tetrapropylammonium iodide (Pr4NI) with a bulky Pr4N+ cation were used to provide the required iodide ion conductivity. Solar cells of configuration FTO/TiO2/Dye/electrolyte/Pt/FTO were fabricated using a mesoporous TiO2 electrode sensitized with a Ruthenium dye (N719). With identical electrolyte compositions, the cells with KI and Pr4NI alone gave efficiencies of 2.37% and 2.90% respectively. The cell with the mixed iodide system, KI:Pr4NI = 16.6:83.4 (% weight ratio), however, showed an enhanced efficiency of 3.92% with a short circuit current density of 9.16 mA cm−2, open circuit voltage of 674.4 mV and a fill factor of 63.4%. 相似文献
6.
Charalambides M. Flegkas P. Pavlou G. Rubio-Loyola J. Bandara A.K. Lupu E.C. Russo A. Dulay N. Sloman M. 《Network and Service Management, IEEE Transactions on》2009,6(1):15-30
Policy-based management provides the ability to (re-)configure differentiated services networks so that desired Quality of Service (QoS) goals are achieved. This requires implementing network provisioning decisions, performing admission control, and adapting bandwidth allocation to emerging traffic demands. A policy-based approach facilitates flexibility and adaptability as policies can be dynamically changed without modifying the underlying implementation. However, inconsistencies may arise in the policy specification. In this paper we provide a comprehensive set of QoS policies for managing Differentiated Services (DiffServ) networks, and classify the possible conflicts that can arise between them. We demonstrate the use of Event Calculus and formal reasoning for the analysis of both static and dynamic conflicts in a semi-automated fashion. In addition, we present a conflict analysis tool that provides network administrators with a user-friendly environment for determining and resolving potential inconsistencies. The tool has been extensively tested with large numbers of policies over a range of conflict types. 相似文献
7.
Sun Lianhong; Petrounia Ioanna P.; Yagasaki Makoto; Bandara Geethani; Arnold Frances H. 《Protein engineering, design & selection : PEDS》2001,14(9):699-704
We have used directed evolution methods to express a fungalenzyme, galactose oxidase (GOase), in functional form in Escherichiacoli. The evolved enzymes retain the activity and substratespecificity of the native fungal oxidase, but are more thermostable,are expressed at a much higher level (up to 10.8 mg/l of purifiedGOase), and have reduced negative charge compared to wild type,all properties which are expected to facilitate applicationsand further evolution of the enzyme. Spectroscopic characterizationof the recombinant enzymes reveals a tyrosyl radical of comparablestability to the native GOase from Fusarium. 相似文献
8.
R.M.G. Rajapakse R.M.M.Y. Rajapakse H.M.N. Bandara B.S.B. Karunarathne 《Electrochimica acta》2008,53(6):2946-2952
Cu(II)-exchanged fuller's earth was prepared by ion-exchanging Ca2+ ions which are present within the interlayer of fuller's earth with Cu(II)ions by the solution-phase ion-exchange process. Pyrrole was introduced into Cu(II)-exchanged fuller's earth to spontaneously polymerize to within the interlayer to result in a nanocomposite of Cu(I)-polypyrrole-fuller's earth where both Cu(I) and polypyrrole occupy within the interlayer spaces of fuller's earth. The nanomaterial [Cu(I)-PPY-FE] has been fully characterized with X-ray diffraction studies, FTIR spectroscopy, DC polarisation test with both blocking stainless steel and non-blocking copper electrodes. The material is found to be a mixed conductor whose ionic mobility is 1.5 times faster than electronic mobility. DC polarisation studies also clearly revealed that the mobile ionic species in this material to be cuprous ions. AC impedance studies have been carried out with blocking stainless steel electrodes at different applied potentials. The necessary theoretical background to explain AC impedance results is also provided and the results obtained agree very well with the corresponding data obtained by other mutually independent methods. The electronic conductivities are around 3.0 × 10−4 S cm−1 and the ionic conductivities are around 9.0 × 10−3 S cm−1. The material may find applications in semi-fuel cells such as air-metal batteries. 相似文献
9.
K.A.T. Amalka Perera S. Gaveshana Anuradha G.R. Asoka Kumara M. Lal Paranawitharana R.M. Gamini Rajapakse H.M.N. Bandara 《Electrochimica acta》2011,(11):19
Design, Development, fabrication and investigation of the I–V characteristics of the DSSC based on interconnected 15 nm SnO2 nanoparticles covered with a nano-scale thin layer of CaCO3 are described. The presence of CaCO3 has been confirmed by its characteristic XRD pattern and EDX plots. The thickness of the protective layer can be conveniently controlled by the molar ratio of SnO2:CaCO3 used in the preparation of the thin film and the optimum conditions for best performance of the DSSC are presented together with possible explanations for the variations observed when the molar ratio is changed. An optimum light-to-electricity conversion efficiency of 5.4% in the presence of a layer of CaCO3 has been obtained which is 3.2 times enhancement over the cell prepared without CaCO3. The characterization of the surface using different techniques is explained. 相似文献
10.