How amantadine and rimantadine inhibit proton transport in the M2 protein channel

To understand how antiviral drugs inhibit the replication of influenza A virus via the M2 ion channel, molecular dynamics simulations have been applied to the six possible protonation states of the M2 ion channel in free form and its complexes with two commercial drugs in a fully hydrated lipid bilayer. Among the six different states of free M2 tetramer, water density was present in the pore of the systems with mono-protonated, di-protonated at adjacent position, tri-protonated and tetra-protonated systems. In the presence of inhibitor, water density in the channel was considerably better reduced by rimantadine than amantadine, agreed well with the experimental IC(50) values. With the preferential position and orientation of the two drugs in all states, two mechanisms of action, where the drug binds to the opening pore and the histidine gate, were clearly explained, i.e., (i) inhibitor was detected to localize slightly closer to the histidine gate and can facilitate the orientation of His37 imidazole rings to lie in the close conformation and (ii) inhibitor acts as a blocker, binding at almost above the opening pore and interacts slightly with the three pore-lining residues, Leu26, Ala30 and Ser31. Here, the inhibitors were found to bind very weakly to the channel due to their allosteric hindrance while theirs side chains were strongly solvated.     

Microarc oxidation in slurry electrolytes: A review

The experience gathered from the use of plasma electrolytic methods in the context of improving the performance of valve group metals is summarized. Emphasis is placed on the formation of coatings by microarc oxidation (MAO) in slurry electrolytes containing powders with different degree of dispersion (a few nanometers to a few tens of microns) and nature (oxides, carbides, nitrides, borides, graphite, etc.). A phenomenological model of the mechanism of formation of MAO coatings in slurry electrolytes is proposed; characteristics of the electrolytes and the coatings are discussed. The results of our studies of the composition of MAO coatings carried out by nuclear backscattering (NBS) spectrum simulation are described. A significant improvement in the properties of MAO coatings formed in slurry electrolytes is registered. It is concluded that this modification can considerably extend the range of application of the MAO method not only in conventional fields of mechanical and instrument engineering but also in aerospace engineering, medicine, biology, and living systems technology.     

Microstructure and mechanical properties of Mg-5Li-1Al sheets prepared by accumulative roll bonding

Ultrafine-grain and high-strength Mg-5Li-1Al sheets were prepared by accumulative roll bonding (ARB) process. Evolution of microstructure and mechanical properties of ARB-processed Mg-5Li-1Al sheets was investigated.Results show that, during ARB process, the evolution of deformation mechanism of t Mg-5Li-1Al alloy is as follows: twinning deformation, shear deformation, forming macro shear zone, and finally dynamic recrystallization (DRX). The grain refining mechanism changes from twin DRX to rotation DRX. With the increase in ARB cycles, strength of the Mg-5Li-1Al sheets is enhanced, whilst elongation varies slightly. With the increase in rolling cycles, anisotropy of mechanical properties decreases. It is conclusive that strain hardening and grain refinement dominate the strengthening mechanism of Mg-5Li-1Al alloy.     

Electrodeposition of nickel–copper alloys to use as a cathode for hydrogen evolution in an alkaline media

The electrocatalytic activity of nickel–copper (Ni–Cu) alloy coated electrodes for the hydrogen evolution reaction (HER) in an alkaline media was studied. The Ni–Cu alloys were electrodeposited on a Cu substrate by direct current (DC) and pulse current (PC) electrodeposition in a fixed plating bath. A wide alloy composition range (6–81 mol% Ni) was achieved by controlling the applied current density between 5 and 300 mA cm⁻². It was found that the electrocatalytic activity for the HER depended on the composition of the Ni–Cu alloys, where electrodes having low Ni content gave high electrocatalytic activities. DC electrodeposition resulted in better electrocatalytic performances than PC. Pulse plating parameters other than the magnitude of the applied current density did not substantially influence the electrocatalytic performance of the Ni–Cu alloy electrodes. Ni content was found to have a stronger effect on the electrocatalytic activity for the HER than the deposit morphology.     

Design and testing of continuous acid-catalyzed esterification reactor for high free fatty acid mixed crude palm oil

Using acid-catalyzed esterification, a continuous reactor, containing four separate continuous stirred tank reactors (CSTR's), was designed and used to reduce the free fatty acid (FFA) content of mixed crude palm oil (MCPO). A six-blade disk turbine and four baffles were installed in each of the four reactors to enhance mixing. The complete reactor was tested using response surface methodology (RSM). A 5-level, 4-factor, central composite design (CCD) was employed to optimize the four important reaction variables (methanol/oil ratio; sulfuric acid/oil ratio; speed of the stirrer; and residence time) to reduce the FFA content of the MCPO to less than 1 wt.% of oil. Multiple regression analysis was used to derive a polynomial equation to predict the FFA content of the product. This was then used to indicate optimal conditions for reducing the FFA in mixed crude palm oil to less than 1 wt.%.     

A novel CMOS charge-pump circuit with current mode control 110 mA at 2.7 V for telecommunication systems

This paper presents a novel organization of switch capacitor charge pump circuits based on voltage doubler structures. Each voltage doubler takes a dc input and outputs a doubled dc voltage. By cascading voltage doublers the output voltage increases up to 2 times. A two-phase voltage doubler and a multiphase voltage doubler structures are discussed and design considerations are presented. A simulator working in the Q-V realm was used for simplified circuit level simulation. In order to evaluate the power delivered by a charge pump, a resistive load is attached to the output of the charge pump and an equivalent capacitance is evaluated. To avoid the short circuit during switching, a clock pairs generator is used to achieve multi-phase non-overlapping clock pairs. This paper also identifies optimum loading conditions for different configurations of the charge pumps. The proposed charge-pump circuit is designed and simulated by spice with TSMC 0.35-μm CMOS technology and operates with a 2.7 V to 3.6 V supply voltage. It has an area of 0.4 mm2; it was designed with a frequency regulation of 1 MHz and internal current mode to reduce power consumption.     

Theoretical and Experimental Analysis of GFRP Bridge Deck under Temperature Gradient

The temperature difference between the top and bottom of a glass fiber reinforced polymer (GFRP) composite deck, ～ 65°C ( ～ 122°F), is nearly three times that of conventional concrete decks ～ 23°C ( ～ 41°F). Such a large temperature difference is attributed to the relatively lower thermal conductivity of GFRP material. In this study, laboratory tests were conducted on two GFRP bridge deck modules (10.2 and 20.3?cm deep decks) by heating and cooling the top surface of the GFRP deck, while maintaining ambient (room) temperature at the deck bottom. Deflections and strains were recorded on the deck under thermal loads. Theoretical results (using macro approach, Navier-Levy, and FEM) were compared with the laboratory test data. The test data indicated that the GFRP deck exhibited hogging under a positive temperature difference (i.e., Ttop>Tbottom, heating test; Ttop and Tbottom are temperatures at top and bottom of the deck, respectively) and sagging under a negative temperature difference (i.e., Ttop相似文献   

Human action and event recognition using a novel descriptor based on improved dense trajectories

Multimedia Tools and Applications - We propose a unified method for recognizing human action and human related events in a realistic video. We use an efficient pipeline of (a) a 3D representation...     

Change in the charge and defect-impurity state of silicon for solar-power engineering under the effect of a magnetic field

The effect of a weak permanent magnetic field on the structure and charge state of silicon for solar-power engineering is investigated. It is revealed that magnetostimulated changes in the defect-impurity state and surface potential have a reversible character.