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1.
Emna Ouni Sbastien Pyr dit Ruys Marie-Madeleine Dolmans Gaëtan Herinckx Didier Vertommen Christiani A. Amorim 《International journal of molecular sciences》2020,21(23)
Currently, the extracellular matrix (ECM) is considered a pivotal complex meshwork of macromolecules playing a plethora of biomolecular functions in health and disease beyond its commonly known mechanical role. Only by unraveling its composition can we leverage related tissue engineering and pharmacological efforts. Nevertheless, its unbiased proteomic identification still encounters some limitations mainly due to partial ECM enrichment by precipitation, sequential fractionation using unfriendly-mass spectrometry (MS) detergents, and resuspension with harsh reagents that need to be entirely removed prior to analysis. These methods can be technically challenging and labor-intensive, which affects the reproducibility of ECM identification and induces protein loss. Here, we present a simple new method applicable to tissue fragments of 10 mg and more. The technique has been validated on human ovarian tissue and involves a standardized procedure for sample processing with an MS-compatible detergent and combined centrifugation. This two-step protocol eliminates the need for laborious sample clarification and divides our samples into 2 fractions, soluble and insoluble, successively enriched with matrisome-associated (ECM-interacting) and core matrisome (structural ECM) proteins. 相似文献
2.
Offline Arabic handwriting recognition: a survey 总被引:1,自引:0,他引:1
3.
Ivan Rodero David Villegas Norman Bobroff Yanbin Liu Liana Fong S. Masoud Sadjadi 《Journal of Grid Computing》2013,11(2):311-336
The goal of Grid computing is to integrate the usage of computer resources from cooperating partners in the form of Virtual Organizations (VO). One of its key functions is to match jobs to execution resources efficiently. For interoperability between VOs, this matching operation occurs in resource brokering middleware, commonly referred to as the meta-scheduler or meta-broker. In this paper, we present an approach to a meta-scheduler architecture, combining hierarchical and peer-to-peer models for flexibility and extensibility. Interoperability is further promoted through the introduction of a set of protocols, allowing meta-schedulers to maintain sessions and exchange job and resource state using Web Services. Our architecture also incorporates a resource model that enables an efficient resource matching across multiple Virtual Organizations, especially where the compute resources and state are dynamic. Experiments demonstrate these new functional features across three distributed organizations (BSC, FIU, and IBM), that internally use different job scheduling technologies, computing infrastructure and security mechanisms. Performance evaluations through actual system measurements and simulations provide the insights on the architecture’s effectiveness and scalability. 相似文献
4.
Energy consumption for developing countries is sharply increasing due to the higher economic growth due to industrialisation along with population growth and urbanisation. The increasing demand of energy leads to global energy crisis. Selecting the best energy technology and conservation requires both quantitative and qualitative evaluation criteria. The fuzzy set-based approach is one of the well-known theories to handle fuzziness, uncertainty in decision-making and vagueness of information. This paper proposes a new method of intuitionistic fuzzy analytic hierarchy process (IF-AHP) to deal with the uncertainty in decision-making. The new IF-AHP is applied to establish a preference in the sustainable energy planning decision-making problem. Three decision-makers attached with Malaysian government agencies were interviewed to provide linguistic judgement prior to analysing with the new IF-AHP. Nuclear energy has been decided as the best alternative in energy planning which provides the highest weight among all the seven alternatives. 相似文献
5.
Liana Franco Padilha Cristine Carretoni Ferreira Fabricio Machado Márcio Nele José Carlos Pinto 《应用聚合物科学杂志》2014,131(9)
In this work, NIRS is employed to provide compositions of α‐olefin copolymers, allowing for evaluation of the effects of certain key process variables on the final NIR spectral responses of obtained polymer materials. This work also introduces a new temperature programmed analytical technique, which combines NIRS measurements with partial fractionation of α‐olefin copolymers. The new proposed technique can be used for evaluation of polyolefin compositions, as presented here for poly(propene/1‐butene) copolymers. Besides, preliminary results obtained from thermal fractionation experiments indicate that this new proposed experimental technique can be employed for characterization of comonomer sequence distributions of α‐olefin copolymers. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014 , 131, 40127. 相似文献
6.
Zimnyakov DA Agafonov DN Sviridov AP Omel'chenko AI Kuznetsova LV Bagratashvili VN 《Applied optics》2002,41(28):5989-5996
Measurements of the contrast value of time-averaged speckle-modulated images of cartilage tissue are used to study tissue thermal modification in the case of laser-light treatment. This modification is related to thermally induced internal stress relaxation in the matrix of the treated tissue. The specific feature of the evolution of time-averaged speckle contrast with a change in the current temperature of modified collagen tissue is the typical looplike form of the contrast-temperature dependencies associated with irreversible changes in tissue structure and correlated with changes in the tissue diffuse transmittance and the tissue internal stress mentioned by other researchers. 相似文献
7.
Machado Liana; Wyatt Natalie; Devine Amy; Knight Benjamin 《Canadian Metallurgical Quarterly》2007,33(5):1045
Humans have a remarkable capability to respond efficiently to a stimulus of interest despite other stimuli competing for neural resources. The current study investigated how the human system copes with distracting stimuli. During each trial, participants viewed 2 sequential stimuli that were each associated with a specific action based on an arbitrary mapping. The 1st stimulus served as a distractor, and the 2nd stimulus required a response (target). When the distractor preceded the target by more than a few hundred milliseconds, response latencies were slower when the 2 stimuli were associated with the same response. The authors propose that this negative compatibility effect stemmed from an inhibitory mechanism that the human system utilizes to prevent the distractor from eliciting an unwanted response. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
8.
Nivia Maria Streit Luisa Helena Rychecki Hecktheuer Marta Weber do Canto Carlos Augusto Mallmann Letícia Streck Thaylise Vey Parodi Liana Pedrolo Canterle 《Food chemistry》2007,102(3):560-564
Erva-mate (Ilex paraguariensis St. Hil.) is a native species from temperate regions of South America, such as Brazil, Paraguay, and Argentina, that is consumed as a beverage known as mate. The objective of this research was to determine the content of caffeic acid, catechin, chlorogenic acid, caffeine, and gallic acid in mate to explain their influence in beverage taste and sensory differences between native and reforested plants, as well as between beverages from plants of different regions of Brazil (Santa Catarina and Rio Grande do Sul states). Compounds were determined by HPLC and results were related to a sensory evaluation performed by trained tasters. Tasters considered the beverage from reforested plants to be more bitter than the beverage from native plants. Beverages from reforested plants had significantly higher caffeic acid and lower catechin, chlorogenic acid, caffeine, and gallic acid content than native plants. Beverages from plants of Santa Catarina state had significantly higher catechin, caffeine, and gallic acid content than plants from Rio Grande do Sul state. 相似文献
9.
10.
Ana Karina Couto Campos Ângela Maria Soares Cardonha Liana Bacurau Galvão Pinheiro Neide Rocha Ferreira Paulo Roberto Medeiros de Azevedo Tânia Lúcia Montenegro Stamford 《Food Control》2009,20(9):807-810
The aim of this study was to assess the hygiene practices of food handlers in municipal schools of Natal, Brazil, where 27 public schools were evaluated, using a checklist and microbiological analysis of hands. It was found that 74.1% of the handlers did not receive periodic training, 51.9% did not undergo annual health examinations and 100% did not practice proper hand hygiene, a situation that reflected significantly (p < 0.05) in hand contamination, in which fecal coliforms were detected on 55.6% of the hands analyzed. It was concluded that the schools studied did not have appropriate hygienic conditions, suggesting the need for interventions that ensure the quality of school food served to the children. 相似文献