Insulinomimetic effect on glucose transport by epidermal growth factor when combined with a major histocompatibility complex class I-derived peptide

Peptides derived from the alpha 1-region of the murine H-2Dk molecule enhance glucose uptake in rat adipose cells above the maximum obtained with insulin stimulation alone (Stagsted, J., Reaven, G. M., Hansen, T., Goldstein, A., and Olsson, L. (1990) Cell 62, 297-307). We now describe that epidermal growth factor (EGF) in combination with the same peptides, Dk-(61-85) and Dk-(62-85), stimulates cellular glucose uptake 5-7 times over the basal level, i.e. to 30-50% of the maximal insulin effect. EGF alone increased glucose uptake by only approximately 50% above basal and the peptide alone by 100% above basal. Maximal effect of EGF and peptide was reached in 10-20 min with 30 microM peptide (EC50 10-15 microM) and 50 nM EGF (EC50 1-2 nM). The effect of EGF and peptide on glucose uptake was additive to that of insulin and peptide until the maximal level attained with insulin and peptide was reached. The combined effect of EGF plus peptide on glucose transport was associated with a recruitment of GLUT4 molecules to the plasma membrane. However, the phosphatidylinositol (PI) kinase which is activated by insulin was not activated by EGF plus peptide. Thus, the effect of EGF plus peptide on glucose uptake seems independent of the activity status of the insulin receptor. 125I-Labeled EGF bound specifically to rat adipose cells with an apparent affinity of approximately 2 nM and Bmax approximately 5 x 10(3). However, the major histocompatibility complex (MHC) peptides did not affect EGF-stimulated internalization of EGF receptor, in contrast to their effect on the insulin receptors. Transforming growth factor alpha had an effect similar to EGF on glucose uptake. Three other peptides derived from other parts of murine MHC class I had no effect on glucose uptake in combination with EGF. Thus, EGF in combination with certain MHC class I-derived peptides is insulinomimetic concerning glucose transport and this effect is independent of the insulin receptor activity.     

System identification from noisy measurements by using instrumental variables and subspace fitting

This paper considers the estimation of the parameters of a linear discrete-time system from noise-perturbed input and output measurements. The conditions imposed on the system are fairly general. In particular, the input and output noises are allowed to be auto-correlated and they may be cross-correlated as well. The proposed method makes use of an instrumental variable (IV)-vector to produce a covariance matrix that is pre- and postmultiplied by some prechosen weights. The singular vectors of the so-obtained matrix possess complete information on the system parameters. A weighted subspace fitting (WSF) method is then applied to the aforementioned singular vectors to consistently estimate the parameters of the system. The IV-WSF technique suggested herein is noniterative and easy to implement, and has a small computational burden. The asymptotic distribution of its estimation errors is derived and the result is used to motivate the choice of the weighting matrix in the WSF step and also to predict the estimation accuracy. Numerical examples are included to illustrate the performance achievable by the method.This work has been supported by the Swedish Research Council of Engineering Sciences under contract 93-669 and by the Göran Gustafsson Foundation.     

Investigations of delamination criticality and the transferability of growth criteria

This article describes a study of delamination growth along 0 °/0 °, 0 °/ 5 °, ± 5 °, and 0 °/90 ° interfaces sandwiched between unidirectional carbon fibre/epoxy composite material. Relationships between damage criticality, growth rate and acoustic emission activity for delamination growth have been studied and the transferability of results from laboratory coupons to composite structural elements has been examined. Two types of coupon tests, conventional delamination beams and rigidly loaded single edge notched strips, have been compared for different mode ratios. Comparative tests have been made on buckling-induced delamination in plates. A graphite crack gauge has been used to measure delamination length and growth rate, ranging from 0.05–2000 m/s. Damage growth was also followed using visual, ultrasonic C-scan, X-ray radiography, macro-video and acoustic emission measurements. Empirical evaluations of interlaminar toughness for delamination beams are made using the Irwin-Kies relation. Unstable growth is analysed using elasto-dynamic moving finite elements. Bucklinginduced delamination is analysed using plate/shell FE methods with growth/remeshing algorithms.     

Fully automatic welding of thick-walled pressure vessels by the narrow gap subarc process

The demands for welding of thick-walled pressure vessels are very stringent. The cost of repairing a weld defect deep down in a weld joint could be very high. As submerged arc welding is considered to be a reliable process for both the welding of pressure vessels and other demanding applications, ESAB chose this process when developing their narrow gap welding technique. This two-pass narrow gap process is described together with the welding and control equipment. The welding results using two types of agglomerated fluxes are presented.     

Signal generation and transmission at 40, 80, and 160 Gb/s using a fiber-optical parametric pulse source

Short return-to-zero pulses (/spl sim/2 ps) are generated at bit rates of 40, 80, and 160 Gb/s using a fiber-optical parametric amplifier. The performance of the parametric pulse source is evaluated both back-to-back and in a 110-km transmission link. A receiver sensitivity of -33 dBm back-to-back was achieved after demultiplexing from 160 to 10 Gb/s. The power penalty at 160 Gb/s due to 110-km transmission was less than 2 dB. Very short pulses (0.5 ps) were also achieved when using the parametric amplifier as a compressor.     

MODE-type algorithm for estimating damped, undamped, or explosive modes

We propose a new algorithm for estimating the parameters of damped, undamped, or explosive sinusoidal signals. The algorithm resembles the MODE algorithm, which is commonly used for direction of arrival estimation in the array signal processing field. It is derived as a natural approximation to an asymptotically (high-SNR) optimal parameter estimator and has excellent statistical accuracy. Nevertheless, it is computationally simple and easy to implement. Numerical examples are included to illustrate the performance of the proposed method.This work has been supported by the Swedish Research Council for Engineering Sciences (TFR).     

Interaction between the Drug Information Centre and the Regional Centre for Adverse Drug Reaction Monitoring in northern Sweden

Two Ghanaian women, aged 21 and 29 years, who were involved in road traffic accidents sustaining blunt trauma to the chest and who had delayed diagnosis of their total bronchial rupture and underwent successful surgical repair 18 and 29 months, respectively after injury are reported with a review of the literature. We believe these to be the first reported cases of delayed diagnosis and successful repair of total bronchial rupture in Tropical Africa.     

Effects of gemfibrozil (Lopid) on hyperlipidemia in acitretin-treated patients. Results of a double-blind cross-over study

Retrospectively 78 patients with uni- or bilateral acute acoustic trauma (AAT) were evaluated to assess the therapeutic effect of hyperbaric oxygenation (HBO). All subjects received saline or dextran (Rheomacodrex) infusions with Ginkgo extracts (Tebonin) and prednisone. Thirty six patients underwent additional hyperbaric oxygenation at a pressure of 2 atmospheres absolute for 60 minutes once daily. Both treatment groups were comparable as far as age, gender, initial hearing loss and prednisone dose are concerned. The delay of therapy onset was 15 hours in both groups and treatment was started within 72 hours in all cases. Control audiometry was performed after 6.5 days, when the HBO group had had 5 exposures to hyperbaric oxygenation. The average hearing gain in the group without HBO was 74.3 dB and in the group treated additionally with HBO 121.3 dB (P < 0.004). It is concluded, that hyperbaric oxygenation significantly improves hearing recovery after AAT. Therefore acute acoustic trauma with significant hearing threshold depression remains an otological emergency. Minimal therapy involving waiting for spontaneous recovery, which is mostly incomplete leaving a residual C5 or C6 and handicapping tinnitus, is not the treatment of choice. Randomized prospective clinical trials with a larger patient series are needed and further experimental studies are required to understand the physiological mechanisms of HBO responsible for the clinical success in AAT.     

Industrial energy efficiency: the need for investment decision support from a manager perspective

Global competition, commitment to the Kyoto Protocol and a deregulated, integrated European electricity market will in all probability increase the demand for energy efficiency on the part of companies in Sweden. Investment decisions are an important part of meeting the new demands, because they decide the future efficiency of industrial energy systems. The objective of this study is to investigate, from a managerial perspective, the need to improve decision support in some industries, which can help to facilitate and improve investment decisions concerning energy efficiency. This work has been conducted through in-depth interviews with representatives for a number of energy-intensive companies and non-energy-intensive companies from different sectors. One need that was identified was the improvement of working methods in order to support the decision-making process. Here, external players seem to be playing an increasingly important role. Access to correct information, better follow-up activities, and transparent, understandable calculations are also considered to be important. The study will form the foundation for subsequent work on decision support and energy efficiency in industry.