Do You Know What I Know? A Shared Understandings Perspective on Text‐Based Communication

This article illustrates how the hermeneutic analysis of text illuminates how shared understandings affect our interpretations of lean communication in distributed work environments. It is proposed that in contrast to the pessimistic conclusions of media richness theory that lean communication channels cannot support complex or equivocal work tasks, miscommunications are not the result of technology, but rather occur due to a lack of shared understandings among the individuals communicating. An illustrative case study based on fieldwork in franchise organizations is presented to demonstrate the possibilities for how the hermeneutic analysis of coherence, invention, intention, and reference can be used to discover how workers create and recreate shared understandings through text.     

Scale-up and control of droplet production in coupled microfluidic flow-focusing geometries

A single microfluidic chip consisting of six microfluidic flow-focusing devices operating in parallel was developed to investigate the feasibility of scaling microfluidic droplet generation up to production rates of hundreds of milliliters per hour. The design utilizes a single inlet channel for both the dispersed aqueous phase and the continuous oil phase from which the fluids were distributed to all six flow-focusing devices. The exit tubing for each of the six flow-focusing devices is separate and individually plumbed to each device. Within each flow-focusing device, the droplet size was monodisperse, but some droplet size variations were observed across devices. We show that by modifying the flow resistance in the outlet channel of an individual flow-focusing device it is possible to control both the droplet size and frequency of droplet production. This can be achieved through the use of valves or, as is done in this study, by changing the length of the exit tubing plumbed to the outlet of the each device. Longer exit tubing and larger flow resistance is found to lead to larger droplets and higher production frequencies. The devices can thus be individually tuned to create a monodisperse emulsion or an emulsion with a specific drop size distribution.     

Deformation and Fracture of Micron‐Sized Metal‐Coated Polymer Spheres: An In Situ Study

In situ imaging and analysis of the mechanical behavior of micron‐sized metal‐coated polymer particles under compression is reported. A nanoindentation set‐up mounted in a scanning electron microscope is used to observe the deformation and fracture of 10 μm polymer spheres with Ni, Ni/Au, Au, and Ag coatings. The spheres fracture in one of two metallization‐dependent modes, brittle, and ductile, depending only on the presence of a nickel layer. The metal coating always fractures parallel to the direction of compression. The mechanical properties up to the point of coating fracture are rate‐dependent due to the viscoelastic polymer core. Metal‐coated polymer spheres are an important composite material in electronics packaging, and this study demonstrates a novel method of evaluating the mechanical properties of particles to tailor them for electronic materials.

     

The provision of online public goods: Examining social structure in an electronic network of practice

Electronic networks of practice are computer-mediated social spaces where individuals working on similar problems self-organize to help each other and share knowledge, advice, and perspectives about their occupational practice or common interests. These interactions occur through message postings to produce an on-line public good of knowledge, where all participants in the network can then access this knowledge, regardless of their active participation in the network. Using theories and concepts of collective action and public goods, five hypotheses are developed regarding the structural and social characteristics that support the online provision and maintenance of knowledge in an electronic network of practice. Using social network analysis, we examine the structure of message contributions that produce and sustain the public good. We then combine the results from network analysis with survey results to examine the underlying pattern of exchange, the role of the critical mass, the quality of the ties sustaining participation, the heterogeneity of resources and interests of participants, and changes in membership that impact the structural characteristics of the network. Our results suggest that the electronic network of practice chosen for this study is sustained through generalized exchange, is supported by a critical mass of active members, and that members develop strong ties with the community as a whole rather than develop interpersonal relationships. Knowledge contribution is significantly related to an individual's tenure in the occupation, expertise, availability of local resources and a desire to enhance one's reputation, and those in the critical mass are primarily responsible for creating and sustaining the public good of knowledge. Finally, we find that this structure of generalized exchange is stable over time although there is a high proportion of member churn in the network.     

The effect of popping,soaking, boiling and roasting processes on antinutritional factors in chickpeas and red kidney beans

This study was conducted to determine the effect that a popping head (like a rice cake machine), a low-shear and low-water processing technology, has on the concentration of antinutritional factors in chickpeas and red kidney beans. Seeds were popped under several parameters (popping time, sample format and equipment type) and analysed against soaking (1:5 w/v in reverse osmosis water for 24 h), roasting (100 g at 180 °C for 20 min) and boiling (1:5 w/v in reverse osmosis water at 100 ± 1 °C for 1 h) processes. Popping and roasting significantly reduced phytic acid content in chickpeas (6%–22%) and red kidney beans (16%–39%). In contrast, phytic acid content after soaking and boiling was not significantly different to raw seeds. Condensed tannins were significantly reduced in red kidney beans after soaking (74%), boiling (100%) and 4 s popping (28%–42%) treatments and increased in both pulses after roasting (137%) and 8 s popping (21%–47%). Further analysis showed that the soluble phenolic content increased with popping, but total and bound phenolic content was reduced. These results demonstrate that the high temperatures and pressures applied during the popping process effectively reduce antinutritional factors in pulses, compared to conventional processing methods.     

Mapping low-Reynolds-number microcavity flows using microfluidic screening devices

Low-Reynolds-number flows in cavities, characterized by separating and recirculating flows are increasingly used in microfluidic applications such as mixing and sorting of fluids, cells, or particles. However, there is still a lack of guidelines available for selecting the appropriate or optimized microcavity configuration according to the specific task at hand. In an effort to provide accurate design guidelines, we investigate quantitatively low-Reynolds-number cavity flow phenomena using a microfluidic screening platform featuring rectangular channels lined with cylindrical cavities. Using particle image velocimetry (PIV), supported by computational fluid dynamics (CFD) simulations, we map the entire spectrum of flows that exist in microcavities over a wide range of low-Reynolds numbers (Re = 0.1, 1, and 10) and dimensionless geometric parameters. Comprehensive phase diagrams of the corresponding microcavity flow regimes are summarized, capturing the gradual transition from attached flow to a single vortex and crossing through two- and three-vortex recirculating systems featuring saddle-points. Finally, we provide design insights into maximizing the rotational frequencies of recirculating single-vortex microcavity systems. Overall, our results provide a complete and quantitative framework for selecting cavities in microfluidic-based microcentrifuges and vortex mixers.     

Hepatic patatin-like phospholipase domain-containing protein 3 sequence,single nucleotide polymorphism presence,protein confirmation,and responsiveness to energy balance in dairy cows

Patatin-like phospholipase domain-containing protein 3 (PNPLA3), commonly known as adiponutrin, is part of a novel subfamily of triglyceride lipase enzymes with potential effects on triglyceride metabolism in adipose and hepatic tissues. The predicted bovine PNPLA3 sequence has been identified, but expression of the gene had not been examined. The objectives of this study were to confirm the predicted bovine PNPLA3 gene sequence, determine expression of the bovine PNPLA3 gene in response to whole-animal energy balance, identify single nucleotide polymorphisms present in dairy cows, and verify the presence of the protein in the liver. Using liver biopsy samples collected from cows at +28 d relative to calving (DRTC), RNA was isolated and used to generate a cDNA template for amplification of the entire predicted coding sequence of PNPLA3 via PCR. To determine if energy balance alters the expression of PNPLA3, RNA was isolated and mRNA expression quantified in liver samples from mid-lactation cows after a 5-d ad libitum period (n = 5) and after a subsequent 5-d 50% feed restriction period (n = 5), and in samples collected from cows at −14, +1, +14, and +28 DRTC (n = 16). The presence of PNPLA3 protein was detected by Western blot in liver protein samples collected at +28 DRTC. Expression of hepatic PNPLA3 was decreased after a period of feed restriction (8.14 vs. 1.08 ± 2.17 arbitrary units, ad libitum vs. fasted). Expression of PNPLA3 mRNA was decreased at +1 and +14 DRTC compared with −14 DRTC (23.35, 7.28, 10.17, and 14.5 ± 4.9 arbitrary units, −14, +1, +14, and +28 DRTC, respectively). The presence of PNPLA3 protein was detected as a 55-kDa band in hepatic protein isolations from liver tissue collected at +28 DRTC. These data confirm the presence and sequence of the bovine hepatic PNPLA3 gene and single nucleotide polymorphisms. Furthermore, these data indicate responsiveness of bovine hepatic PNPLA3 to energy balance.     

Cone Photoreceptor Loss in Light-Damaged Albino Rats

We investigated the etiology of decreased cone-driven vision in a light damage (LD) model of retinal degeneration. To induce slow, moderate degeneration, albino rats underwent low-intensity light exposure for 10 days. Electroretinography was utilized to assess physiologic function of the rod- and cone-driven retinal function in LD and control rats. Immunohistochemistry targeting cone arrestin allowed for quantification of cone density and for comparison of the decline in function. Photoreceptor loss was quantified by outer nuclear layer thickness decreases, as observed by optical coherence tomography and histology. The LD rats showed decreased rod- and cone-driven function with partial recovery 30 days after cessation of light exposure. In addition, LD rats showed decreased cone photoreceptor densities in the central retinal region compared to control rats. Our results demonstrate that the loss of cone-driven visual function induced by light damage is at least partially due to the death of cone photoreceptors.     

Fluorescence tracking of dissolved and particulate organic matter quality in a river-dominated estuary

Excitation-emission matrix (EEM) fluorescence was combined with parallel factor analysis (PARAFAC) to model base-extracted particulate (POM) and dissolved (DOM) organic matter quality in the Neuse River Estuary (NRE), North Carolina, before and after passage of Hurricane Irene in August 2011. Principle components analysis was used to determine that four of the PARAFAC components (C1-C3 and C6) were terrestrial sources to the NRE. One component (C4), prevalent in DOM of nutrient-impacted streams and estuaries and produced in phytoplankton cultures, was enriched in the POM and in surface sediment pore water DOM. One component (C5) was related to recent autochthonous production. Photoexposure of unfiltered Neuse River water caused an increase in slope ratio values (S(R)) which corresponded to an increase in the ratio C2:C3 for DOM, and the production of C4 fluorescence in both POM and DOM. Changes to the relative abundance of C4 in POM and DOM indicated that advection of pore water DOM from surface sediments into overlying waters could increase the autochthonous quality of DOM in shallow microtidal estuaries. Modeling POM and DOM simultaneously with PARAFAC is an informative technique that is applicable to assessments of estuarine water quality.