Purification of a tumoral marker recognized by monoclonal antibody Po66 and associated with human lung squamous cell carcinoma

This paper reports on the production of tumor necrosis factor (TNF) and granulocyte macrophage colony-stimulating factor (GM-CSF) by cultured mononuclear adherent cells derived from bone marrow of 25 patients affected by myelodysplastic syndrome (MDS) of different FAB subtypes. Mean production of GM-CSF was much lower than in controls, without significant differences among different subtypes. Mean production of TNF was similar in MDS patients and in controls, but noteworthy differences were observed between patients with RA, RAEB and RAEB-t and patients with RARS and CMML. Growth of bone marrow granulocyte macrophage and erythroid progenitors did not correlate with TNF and GM-CSF production, although in MDS subtypes with higher GM-CSF levels, colony growth was slightly higher than in subtypes with lower GM-CSF production.     

Polyunsaturated fatty acids in tissues of rats fed trielaidin and high or low levels of linolenic acid

Male and female weanling rats that were born to dams fed a diet low in linolenic acid received diets of 15% lipids by weight containing 45% elaidic acid (as trielaidin) and 8.5% or 0.1% linolenic acid for 10 weeks. Four other groups, in which palmitic or oleic acid replaced elaidic acid in the diet, served as controls. The fatty acid profiles of several lipid classes were determined in adipose tissue, adrenals, testes, heart and brain. Elaidic acid was incorporated into tissue lipids in varying degrees, depending on the organ and on the lipid class. Feeding elaidic acid induced no changes in the polyunsaturated fatty acid (PUFA) profiles of testes lipids but resulted in definite modifications of the PUFA patterns of heart phosphatidylcholine (PC) and phosphatidylethanolamine (PE). In linolenic acid-deprived rats, arachidonic acid was decreased in PC and linoleic acid was increased in both PC and PE; 22∶5n−6 was strongly depressed in both PC and PE. In linolenic acid-fed rats, 22∶6n−3 was decreased in PC and PE. These changes, on the whole, were more evident in females, and some also were observed in adrenal cholesteryl esters but only slightly in brain phospholipids. the apparent inhibition of the biosynthesis of PUFA induced by dietary elaidic acid appeared to be complex and of greater intensity in the n−6 fatty acid series than in their n−3 homologues.     

Development of the Local Approach to Fracture over the Past 25 years: Theory and Applications

This review paper is devoted to the local approach to fracture (LAF) for the prediction of the fracture toughness of structural steels. The LAF has been considerably developed over the past two decades, not only to provide a better understanding of the fracture behaviour of materials, in particular the failure micromechanisms, but also to deal with loading conditions which cannot easily be handled with the conventional linear elastic fracture mechanics and elastic–plastic fracture mechanics global approaches. The bases of this relatively newly developed methodology are first presented. Both ductile rupture and brittle cleavage fracture micromechanisms are considered. The ductile-to-brittle transition observed in ferritic steels is also briefly reviewed. Two types of LAF methods are presented: (i) those assuming that the material behaviour is not affected by damage (e.g. cleavage fracture), (ii) those using a coupling effect between damage and constitutive equations (e.g. ductile fracture). The micromechanisms of brittle and ductile fracture investigated in elementary volume elements are briefly presented. The emphasis is laid on cleavage fracture in ferritic steels. The role of second phase particles (carbides or inclusions) and grain boundaries is more thoroughly discussed. The distinction between nucleation and growth controlled fracture is made. Recent developments in the theory of cleavage fracture incorporating both the effect of stress state and that of plastic strain are presented. These theoretical results are applied to the crack tip situation to predict the fracture toughness. It is shown that the ductile-to-brittle transition curve can reasonably be well predicted using the LAF approach. Additional applications of the LAF approach methods are also shown, including: (i) the effect of loading rate and prestressing; (ii) the influence of residual stresses in welds; (iii) the mismatch effects in welds; (iv) the warm-prestressing effect. An attempt is also made to delineate research areas where large improvements should be made for a better understanding of the failure behaviour of structural materials.     

Ductile rupture in thin sheets of two grades of 2024 aluminum alloy

The damage and rupture mechanisms of thin sheets of 2024 aluminum alloy (Al containing Cu, Mn, and Mg elements) are investigated. Two grades are studied: a standard alloy and a high damage tolerance alloy. The microstructure of each material is characterized to obtain the second phase volume content, the dimensions of particles and the initial void volume fraction. The largest particles consist of intermetallics. Mechanical tests are carried out on flat specimens including U-notched (with various notch radii), sharply V-notched and smooth tensile samples. Stable crack growth was studied using “Kahn samples” and pre-cracked large center-cracked tension panels M(T). The macroscopic fracture surface of the different specimens is observed using scanning electron microscopy. Smooth and moderately notched samples exhibit a slant fracture surface, which has an angle of about 45° with respect to the loading direction. With increasing notch severity, the fracture mode changes significantly. Failure initiates at the notch root in a small triangular region perpendicular to the loading direction. Outside this zone, slant fracture is observed. Microscopic observations show two failure micromechanisms. Primary voids are first initiated at intermetallic particles in both cases. In flat regions, i.e. near the notch root of severely notched samples, void growth is promoted and final rupture is caused by “internal necking” between the large cavities. In slanted regions these voids tend to coalesce rapidly according to a “void sheet mechanism” which leads to the formation of smaller secondary voids in the ligaments between the primary voids. These observations can be interpreted using finite element simulations. In particular, it is shown that crack growth occurs under plane strain conditions along the propagation direction.     

Can distributed generation offer substantial benefits in a Northeastern American context? A case study of small-scale renewable technologies using a life cycle methodology

Renewable distributed electricity generation can play a significant role in meeting today's energy policy goals, such as reducing greenhouse gas emissions, improving energy security, while adding supply to meet increasing energy demand. However, the exact potential benefits are still a matter of debate. The objective of this study is to evaluate the life cycle implications (environmental, economic and energy) of distributed generation (DG) technologies. A complementary objective is to compare the life cycle implications of DG technologies with the centralized electricity production representing the Northeastern American context. Environmental and energy implications are modeled according to the recommendations in the ISO 14040 standard and this, using different indicators: Human Health; Ecosystem Quality; Climate Change; Resources and Non-Renewable Energy Payback Ratio. Distinctly, economic implications are modeled using conventional life cycle costing. DG technologies include two types of grid-connected photovoltaic panels (3 kWp mono-crystalline and poly-crystalline) and three types of micro-wind turbines (1, 10 and 30 kW) modeled for average, below average and above average climatic conditions in the province of Quebec (Canada). A sensitivity analysis was also performed using different scenarios of centralized energy systems based on average and marginal (short- and long-term) technology approaches. Results show the following. First, climatic conditions (i.e., geographic location) have a significant effect on the results for the environmental, economic and energy indicators. More specifically, it was shown that the 30 kW micro-wind turbine is the best technology for above average conditions, while 3 kWp poly-crystalline photovoltaic panels are preferable for below average conditions. Second, the assessed DG technologies do not show benefits in comparison to the centralized Quebec grid mix (average technology approach). On the other hand, the 30 kW micro-wind turbine shows a potential benefit as long as the Northeastern American electricity market is considered (i.e., oil and coal centralized technologies are affected for the short- and long-term marginal scenarios, respectively). Photovoltaic panels could also become more competitive if the acquisition cost decreased. In conclusion, DG utilization will represent an improvement over centralized electricity production in a Northeastern American context, with respect to the environmental, energy and economic indicators assessed, and under the appropriate conditions discussed (i.e., geographical locations and affected centralized electricity production scenarios).     

Micromechanical modeling of the behavior of duplex stainless steels

The aim of the present paper is the micromechanical modeling of an aged duplex (austenite/ferrite) stainless steel. While the elastic properties of both phases are almost the same, the plastic mismatch between ferrite and austenite is high due to ferrite aging. Accounting for the complex micro and macrostructure of this material, three scales are investigated. The first one corresponds to each single phase. The second scale consists of a percolated (interwoven) network of both phases. The third scale is an aggregate of percolated bicrystals. For the first scale, each phase is modeled as a single crystal. In the second scale, F.E. simulations are performed on a unit cubic cell representing the percolated networks. A mean field model is then fitted in order to represent that bicrystal. This mean field model is used at the third scale to model the behavior of the aggregate of two-phase grains, using a model for multi-phase materials. At this scale, it becomes possible to represent the distribution of average ferrite stresses in each ferrite-austenite bicrystal. These variations are thought to be responsible for the heterogeneous damage nucleation observed in this material.     

Impact fracture of a ferritic steel in the lower shelf regime

This paper reports the results of a systematic investigation on the fracture of Charpy-V notch A508 steel specimens, tested in the lower shelf regime. The fracture energy has been determined for quasi-static, standard Charpy and one-point-bend impact. The results show a general trend for the fracture energy to increase with the loading rate, at the lower temperature (–160 °C). At this temperature, the roughness of the fracture surface increases markedly with the loading rate. The fractographic analysis shows the presence of 3–4 cleavage initiation sites situated at 100–800 m from the crack front, irrespective of the loading rate. Numerous cleavage microcracks are observed underneath the main fracture plane. The statistical analysis shows that the length distribution of the microcracks is adequately described by Weibull statistics. It is also found that the number of microcracks increases with the loading rate. It is suggested that the larger number of microcracks is responsible for the observed increased roughness and energy dissipation.     

Corium spreading and coolability: CSC Project

In the context of severe accidents, large R&D efforts throughout the world are currently directed towards ex-vessel corium behaviour. Among the mitigation means which can be envisaged, the European industries and utilities are considering the implementation of a core-catcher outside the reactor pressure vessel in order to prevent basemat erosion and to stabilize and control the corium within the containment. The CSC project focused on two key phenomena for external core-catcher efficiency, reliability and safety: spreading and coolability. An experimental programme, covering different scenarios and including both simulant and real materials provided a lot of results which now constitute a large database and which enabled the qualification of computer codes.     

Efficacy and environmental acceptability of two ballast water treatment chemicals and an alkylamine based-biocide

Ship's ballast waters transport large numbers of organisms which may become invasive in coastal regions. One option to address this problem is the use of biocides as ballast water treatment (BWT). Efficacy and environmental acceptability of three commercial active substances (the BWT biocides Peraclean^® Ocean and Seakleen^®, and alkylamine-based biocide Mexel^® 432/336) were tested against three bacteria species, two vegetative microalgae and one zooplanktonic larva, in 10 and 30 Practical Salinity Unit (PSU) waters. In both salinities, PeraClean^® Ocean was the most effective biocide against bacteria causing > 90% mortality at 20 mg/l, compared with 50 mg/l for Mexel^® 432/336 and > 500 mg/l for Seakleen^®. Regarding zooplankton, Seakleen^® was the most effective chemical causing 90% mortality in 24 h at concentrations < 6 mg/l (LC90_24 h) in both salinities, compared with 23 and 26 mg/l for Mexel^® 432/336 and 370 and 480 mg/l for PeraClean^® Ocean in 10 and 30 PSU, respectively. Similar pattern of efficacy was obtained for microalgae in 30 PSU: effective concentrations inducing 50% growth inhibition in 4 days were ≤ 1.6 mg/l for Seakleen^®, ≤ 10.1 mg/l for Mexel^® 432/336 and ≤ 30.9 mg/l for PeraClean^® Ocean. Our work highlighted that treated waters displayed residual toxicity after 24 h still inducing mortality depending on the organism and biocide. However Mexel^® 432/336 is the only biocide which had no impact on oyster larvae development at effective concentration. Altogether our data showed that Mexel^® 432/336 was the only biocide displaying a broad spectrum efficacy in concentrations < 50 mg/l and not toxic for oyster larvae development at this concentration. However residual toxicity of treated waters for any organism should be taken into account in BWT systems utilising biocides.