An illustration of verification and validation in the modelling phase of KBS development

Reliability has become a key factor in KBS development. For this reason, it has been suggested that verification and validation (V&V) should become an integrated part of activities throughout the whole KBS development cycle. In this paper, it will be illustrated how the PROLOGA workbench integrates V&V aspects into its modelling environment, such that these techniques can be of assistance in the process of knowledge acquisition and representation. To this end, verification has to be performed incrementally and can no longer be delayed until after the system has been completed. It will be shown how this objective can be realised through an approach that uses the decision table formalism as a modelling instrument.     

Determination of furazolidone residues in eggs by HPLC followed by confirmation with a diode-array UV/Vis detector

A sensitive HPLC method for the determination of furazolidone residues in eggs (10-1,000 micrograms/kg) is described. Recovery is about 86%. With the aid of a UV/Vis Diode-Array detector confirmation up to the 15-ppb level was possible. In order to test this method with "real" samples, three laying hens received 30 mg each of furazolidone in feed (single dose). The eggs were collected for five days. After five days traces of furazolidone (5 micrograms/kg) could still be detected.     

GCase and LIMP2 Abnormalities in the Liver of Niemann Pick Type C Mice

The lysosomal storage disease Niemann–Pick type C (NPC) is caused by impaired cholesterol efflux from lysosomes, which is accompanied by secondary lysosomal accumulation of sphingomyelin and glucosylceramide (GlcCer). Similar to Gaucher disease (GD), patients deficient in glucocerebrosidase (GCase) degrading GlcCer, NPC patients show an elevated glucosylsphingosine and glucosylated cholesterol. In livers of mice lacking the lysosomal cholesterol efflux transporter NPC1, we investigated the expression of established biomarkers of lipid-laden macrophages of GD patients, their GCase status, and content on the cytosol facing glucosylceramidase GBA2 and lysosomal integral membrane protein type B (LIMP2), a transporter of newly formed GCase to lysosomes. Livers of 80-week-old Npc1^−/− mice showed a partially reduced GCase protein and enzymatic activity. In contrast, GBA2 levels tended to be reciprocally increased with the GCase deficiency. In Npc1^−/− liver, increased expression of lysosomal enzymes (cathepsin D, acid ceramidase) was observed as well as increased markers of lipid-stressed macrophages (GPNMB and galectin-3). Immunohistochemistry showed that the latter markers are expressed by lipid laden Kupffer cells. Earlier reported increase of LIMP2 in Npc1^−/− liver was confirmed. Unexpectedly, immunohistochemistry showed that LIMP2 is particularly overexpressed in the hepatocytes of the Npc1^−/− liver. LIMP2 in these hepatocytes seems not to only localize to (endo)lysosomes. The recent recognition that LIMP2 harbors a cholesterol channel prompts the speculation that LIMP2 in Npc1^−/− hepatocytes might mediate export of cholesterol into the bile and thus protects the hepatocytes.     

Bayesian assessment of the characteristic concrete compressive strength using combined vague-informative priors

Bayesian statistics can be used in order to determine the characteristic value of an unknown distribution based on a limited number of test samples. In cases where no previous test results are available, most often a Bayesian method based on vague prior information is used. The assumption of a vague or uniform prior results in a conservative approach in cases where only a limited number of test results are available. However, in case of concrete, prior information on concrete strength can be found in literature or country-specific prior information can be determined. Therefore, the use of a combined vague-informative prior is of particular interest, more specifically in the form of scaled inverse-χ² distributions that can be used for updating the standard deviation of the strength distribution of concrete. The differences between the use of a vague and a combined vague-informative prior are investigated through Monte Carlo simulations. Because prior information is taken into account, the uncertainty regarding the standard deviation of the predictive strength distribution is significantly reduced.     

Project affinity: the role of emotional attachment in construction projects

Although a wide range of human and organizational factors have been found to be important in the operation of projects, those determined by cultural variables are less well defined. One such influence concerns the notion of ‘project affinity’, the commitment and attachment by stakeholders and participants to projects and their outcomes. The temporal nature and transient involvement context provided by construction projects arguably presents a climate in which many participants are less likely to display commitment to its goals. This note is concerned with the issue of whether an attachment to a project's goals or to the completed product can lead to improved commitment among those involved. The concept of project affinity was developed in the course of a case study investigation in which operatives' attitudes towards the construction of a cancer research facility were explored. Because of the contribution of the facility to the future care of cancer sufferers, the concept of project affinity proved helpful in understanding a factor that appeared to be influencing the commitment of those involved. The results suggest a complementary concept to that of ‘project chemistry’ (c.f. Nicolini, 2002 Nicolini, D. 2002. In search of ‘project chemistry’.. Construction Management and Economics, 20: 167–77.  ).     

Multi-level Modeling of Silica–Template Interactions During Initial Stages of Zeolite Synthesis

Zeolite synthesis is driven by structure-directing agents, such as tetrapropyl ammonium ions (TPA⁺) for Silicalite-1 and ZSM-5. However, the guiding role of these organic templates in the complex assembly to highly ordered frameworks remains unclear, limiting the prospects for advanced material synthesis. In this work, both static ab initio and dynamic classical modeling techniques are employed to provide insight into the interactions between TPA⁺ and Silicalite-1 precursors. We find that as soon as the typical straight 10-ring channel of Silicalite-1 or ZSM-5 is formed from smaller oligomers, the TPA⁺ template is partially squeezed out of the resulting cavity. Partial retention of the template in the cavity is, however, indispensable to prevent collapse of the channel and subsequent hydrolysis.

Probabilistic evaluation of conformity criteria for concrete families

When only a limited number of test results are available, compressive strength results of concrete family members can be combined in order to enable conformity control on a sufficient number of test results. The principles behind the conformity control of concrete families are explained and an original probabilistic evaluation of conformity criteria for concrete families is introduced. For the calculation of the corresponding operating characteristics, some approximate formulae are presented. Additionally, Monte Carlo simulation techniques are used for more exact calculations. In this way, also autocorrelation between consecutive test results can be taken into account, using an autoregressive process. The current guidelines and conformity criteria for concrete families in EN 206-1 are evaluated and discussed, based on the AOQL concept. Different conformity criteria and transformation methods are described, evaluated and discussed.     

Uncertainties in the fate of nitrogen I: An overview of sources of uncertainty illustrated with a Dutch case study

This study focuses on the uncertainties in the fate of nitrogen (N) in the Netherlands. Nitrogen inputs into the Netherlands in products, by rivers, and by atmospheric deposition, and microbial and industrial fixation of atmospheric N₂ amount to about 4450 Gg N y^–1. About 60% of this N is transported out of the Netherlands in products. The fate of the remaining 40%, however, is less clear. We discuss uncertainties in losses to the atmosphere (as ammonia or through denitrification), by leaching and runoff, and in N accumulation in biomass and soils. These processes may account for the fate of about 40% of the N in the Netherlands, and for the fate of about 60% of the N in Dutch agricultural soils. Reducing uncertainties in the estimates of these fluxes is necessary for reducing the impact of excess N in the environment. In particular, monitoring the environmental effects of ammonia emissions and nitrate leaching to groundwater and aquatic systems requires an increased understanding of the fate of N. Uncertainties arise because (1) some N fluxes cannot be measured directly and are usually quantified indirectly as the balance in N budgets, (2) direct measurements of N fluxes have inevitable inaccuracies, (3) lack of experimental data and other information (e.g. statistics) needed for upscaling, (4) large spatial and temporal variability of fluxes, and (5) poor understanding of the processes involved. These uncertainties can be reduced by additional experimental studies and by further development of process-based models and N budget studies. We prioritize these future research needs according to a range of different criteria.