Identification of metal ion solutions using acoustic plate mode devices and pattern recognition

The temperature dependence of acoustic plate mode (APM) devices used as probes for dilute electrolytes is described. Specifically, the probe responses that consist of the frequency change and device loss were studied for dilute aqueous solutions of alkali metal ions. It is shown that by integrating the temperature dependence of the APM probe responses with pattern recognition techniques, valuable information about the solutions can be obtained that include identification and quantification. A preliminary investigation of the feasibility of identifying binary mixtures of the alkali metal ion solutions using only the temperature responses showed good results.     

Making and negotiating value: design and collaboration with community led groups

ABSTRACT

Value in collaborative design research and practice can be understood fundamentally as relationships, materials, processes, contexts, and outcomes that are subjects of and for negotiation. We argue for conceptions of value that move beyond traditional ‘outcomes’ based measurements to reimagine and rearticulate value itself as co-created, emerging from negotiation, relationality and immersion in specific contexts. These understandings of value, we argue, are not rooted in or always knowable through designers’ experiences, even as designers participate in creating them. Using case studies from our research we suggest that value in design collaboration emerges as a question: value to whom, and to what end? We propose that addressing these questions ethically through co-design requires actively engaged, grounded work with collaborators based in three principles: being present for the work, participant making, and co-creating capacity for collaborators to ‘go off and do their thing’.     

生活方式视角下获得性下肢残疾青年轮椅体验设计

目的 源于对获得性下肢残疾青年群体社会融入问题的关注,探讨其生活方式转变与轮椅设计需求的关系,通过体验设计的提升,促进目标群体生活信心的重建。方法 在理解获得性下肢残疾青年群体特征和社会融入问题的基础上,结合网络文本分析和用户访谈结果,改良AIOD生活方式测量模型,建立该群体生活方式的测量维度。基于测量模型维度,进行问卷调查,通过统计分析建构该群体的生活方式并进行细分。以生活方式测量结果为依据,结合CUE体验设计框架,输出轮椅体验设计机会点。结果 研究发现获得性下肢残疾青年群体的生活方式可细分为积极乐观型、安稳生活型、消极懈怠型,从包容各类型方式的角度出发提出适应多元化生活方式的轮椅设计策略,进行可控性、有效性、易用性、感知性和认可性等方面的优化轮椅体验,满足生活转型产生的新需求,进一步促进生活转型积极心理的形成。     

Multisensor Data Product Fusion for Aerosol Research

Combining data sets from multiple satellite sensors is a powerful method for studying Earth-atmosphere problems. By fusing data, we can utilize the strengths of the individual sensors that may not be otherwise possible. In this paper, we provide the framework for combining level 2 data products, using data from three sensors aboard the National Aeronautics and Space Administration (NASA)'s Terra satellite. These data include top-of-the-atmosphere (TOA) radiative energy fluxes obtained from the Clouds and the Earth's Radiant Energy System (CERES), aerosol optical thickness from the multispectral Moderate Resolution Imaging Spectroradiometer (MODIS), and aerosol properties from the Multi-angle Imaging SpectroRadiometer (MISR). The CERES Single Scanner Footprint (SSF) contains the pixel level CERES TOA fluxes and the level 2 MODIS aerosol data. We specifically focus upon fusing the CERES SSF with the MISR aerosol products. Although this project was undertaken specifically to address aerosol research, the methods employed for fusing data products can be used for other problems requiring synergistic data sets. We present selected case studies over different aerosol regimes and indicate that multisensor information provides value-added information for aerosol research that is not available from a single sensor.     

Mass customization in the product life cycle

This study presents an introduction to mass customization in the product life cycle—the goal of mass customization, mass customization configurations, and new customer integration techniques, modular design techniques, flexible manufacturing systems (FMSs), and supply chain management methods. The study reviews three selected books and twenty-one selected papers—early papers that describe the goal of mass customization, early papers that describe mass customization configurations, and recent papers that describe new customer integration techniques, modular design techniques, FMSs, and supply chain management methods. The study shows that the goal of mass customization is to create individually customized products, with mass production volume, cost, and efficiency, that most companies use ‘assemble-to-order’ configurations to create standardized products, and that more work is needed on interactive customer integration techniques, collaborative modular design techniques, reconfigurable manufacturing systems, and integrated supply chain management methods to achieve the goal of mass customization.     

Circulating Tumor Cells (CTC) and Cell-Free DNA (cfDNA) Workshop 2016: Scientific Opportunities and Logistics for Cancer Clinical Trial Incorporation

Despite the identification of circulating tumor cells (CTCs) and cell-free DNA (cfDNA) as potential blood-based biomarkers capable of providing prognostic and predictive information in cancer, they have not been incorporated into routine clinical practice. This resistance is due in part to technological limitations hampering CTC and cfDNA analysis, as well as a limited understanding of precisely how to interpret emergent biomarkers across various disease stages and tumor types. In recognition of these challenges, a group of researchers and clinicians focused on blood-based biomarker development met at the Canadian Cancer Trials Group (CCTG) Spring Meeting in Toronto, Canada on 29 April 2016 for a workshop discussing novel CTC/cfDNA technologies, interpretation of data obtained from CTCs versus cfDNA, challenges regarding disease evolution and heterogeneity, and logistical considerations for incorporation of CTCs/cfDNA into clinical trials, and ultimately into routine clinical use. The objectives of this workshop included discussion of the current barriers to clinical implementation and recent progress made in the field, as well as fueling meaningful collaborations and partnerships between researchers and clinicians. We anticipate that the considerations highlighted at this workshop will lead to advances in both basic and translational research and will ultimately impact patient management strategies and patient outcomes.     

Fluorescent THF‐Based Fructose Analogue Exhibits Fructose‐Dependent Uptake

Recent publications suggest that high dietary fructose might play a significant role in cancer metabolism and can exacerbate a number of aspects of metabolic syndrome. Addressing the role that fructose plays in human health is a controversial question and requires a detailed understanding of many factors including the mechanism of fructose transport into healthy and diseased cells. Fructose transport into cells is thought to be largely mediated by the passive hexose transporters Glut2 and Glut5. To date, no probes that can be selectively transported by one of these enzymes but not by the other have been identified. The data presented here indicate that, in MCF‐7 cells, a 1‐amino‐2,5‐anhydro‐D ‐mannitol‐based fluorescent NBDM probe is transported twice as efficiently as fructose and that this takes place with the aid of Glut5. Its Glut5 specificity and differential uptake in cancer cells and in normal cells suggest this NBDM probe as a potentially useful tool for cross‐cell‐line correlation of Glut5 transport activity.     

Allele-Specific Inhibition of Histone Demethylases

Histone demethylases play a critical role in mammalian gene expression by removing methyl groups from lysine residues in degree- and site-specific manner. To specifically interrogate members and isoforms of this class of enzymes, we have developed demethylase variants with an expanded active site. The mutant enzymes are capable of performing lysine demethylation with wild-type proficiency, but are sensitive to inhibition by cofactor-competitive molecules embellished with a complementary steric “bump”. The selected inhibitors show more than 20-fold selectivity over the wild-type demethylase, thus overcoming issues typical to pharmacological and genetic approaches. The mutant–inhibitor pairs are shown to act on a physiologically relevant full-length substrate. By engineering a conserved amino acid to achieve member-specific perturbation, this study provides a general approach for studying histone demethylases in diverse cellular processes.