Studies on the dechlorination and detoxificaton of organochlorine compounds. I. PCBs and PCNs

In the analysis of organochlorines such as polychlorinated biphenyls (PCBs) and polychlorinated naphthalenes (PCNs), levels of, for example, PCB concentration are often expressed as a ‘total PCB’ value, using a straight GC pattern-matching technique with electron capture detection. Such methods provide little information about the identity of the individual components. In the present study, preliminary investigations using dechlorination with a NiCl₂/NaBH₄ catalyst indicated that the technique offered a comprehensive method for the analysis of PCBs and PCNs, as well as a method of detoxification of the ‘priority pollutants’ . This paper reports the use of capillary column GC-MS with an ion trap detector (ITD^TM) for the analysis of a number of PCBs and PCNs, including the commercially produced Aroclor 1254 and 1221, employing this dechlorination technique.     

Effects of international collaboration and status of journal on impact of papers

This study examines the effect of international collaboration of Slovenian authors and the status of journals where papers are published (as determined by their impact factors) on the impact of papers as measured by the number of citations papers receive. Research programme groups working in Slovenia in the 2004–2008 period in the fields of physics, chemistry, biology, biotechnology, and medical science were used for analyses. The results of the analyses show that the effects of the two factors differ among the fields. We discuss possible reasons for this, including the possibility that differences are the result of Slovenia’s science policy.     

Preparation of copolymers of p-Isopropenylcalix[8]arene and Styrene

Summary p-Isopropenylcalix[n]arenes are useful building blocks for the design of novel polymer architectures. This paper is the first report on copolymerization of p-isopropenylcalix[8]arene and styrene by free radical polymerization. An extensive study on the optimization of the reaction conditions (especially monomer to initiator ratio, initiator concentration, and duration of the process) was performed in order to prepare high molecular weight copolymers in good yield. Homopolymerizations were also carried out for comparison. The products obtained by homopolymerization of the monomers and their copolymerization were fractionated and each fraction was characterized by chromatographic (GPC and HLPC) and spectroscopic methods as UV, IR and ¹H NMR and analytical methods. Considering the molecular weight, spectroscopic and analytical data the most probable structure of the copolymer was proposed. Extraction of metal picrates from water into organic solvent was used to evaluate the ion binding capabilities of p-isopropenylcalix[8]arenestyrene and O-acetylated p-isopropenycalix[8]arenestyrene copolymers.     

Hologram reconstruction corrected for measurements through layers with different refractive indices in digital in-line holographic microscopy

Digital in-line holographic microscopy (DIHM) using point sources has been shown to be a versatile technique, especially for three-dimensional tracking of particles or microorganisms. However, the spherical source wave is altered when measurements are performed through layers with different refractive indices, such as water cuvettes. The situations where a layer of medium with a refractive index different than that of the predominant surrounding propagation medium (usually air) is situated behind or in front of the plane to be reconstructed are analyzed in detail, and a general approach for reconstruction under such circumstances is developed. The proposed refractive index correction is tested experimentally and compared to conventional reconstruction algorithms. Using 3D traces of swimming algal spores, the influence on the velocity calculation is also shown.     

The influence of an organosulfonate on the reaction between Ellman's reagent and thiocholine

The aim of this work was to elucidate the effect of methanesulfonyl fluoride on the detection reaction for the determination of cholinesterase activity. The effect of methanesulfonyl fluoride was studied by monitoring the time course of the appearance and disappearance of the detection reaction product using a stopped-flow technique. The obtained experimental data were analyzed by progress curve analysis. It was found that the effect of methanesulfonyl fluoride results from a reaction between methanesulfonyl fluoride and the detection reaction product with the following rate constant: k1 = 5.33 x 10(-2) M-1s-1. In evaluating the effect of methanesulfonyl fluoride, the decomposition of this agent in water was included in the analysis because the decomposition considerably affected the reaction between methanesulfonyl fluoride and the product. The corresponding rate constant was found to be k2 = 4.9 x 10(-5) M-1s-1.     

Determinization of fuzzy automata with membership values in complete residuated lattices

In this paper we introduce a new method for determinization of fuzzy finite automata with membership values in complete residuated lattices. In comparison with the previous methods, developed by Bělohlávek [R. Bělohlávek, Determinism and fuzzy automata, Information Sciences 143 (2002), 205-209] and Li and Pedrycz [Y.M. Li, W. Pedrycz, Fuzzy finite automata and fuzzy regular expressions with membership values in lattice ordered monoids, Fuzzy Sets and Systems 156 (2005), 68-92], our method always gives a smaller automaton, and in some cases, when the previous methods result in infinite automata, our method can result in a finite one. We also show that determinization of fuzzy automata is closely related to fuzzy right congruences on a free monoid and fuzzy automata associated with them, and in particular, to the concept of the Nerode’s fuzzy right congruence of a fuzzy automaton, which we introduce and study here.     

Molecular and Clinical Implications of Variant Repeats in Myotonic Dystrophy Type 1

Myotonic dystrophy type 1 (DM1) is one of the most variable monogenic diseases at phenotypic, genetic, and epigenetic level. The disease is multi-systemic with the age at onset ranging from birth to late age. The underlying mutation is an unstable expansion of CTG repeats in the DMPK gene, varying in size from 50 to >1000 repeats. Generally, large expansions are associated with an earlier age at onset. Additionally, the most severe, congenital DM1 form is typically associated with local DNA methylation. Genetic variability of DM1 mutation is further increased by its structural variations due to presence of other repeats (e.g., CCG, CTC, CAG). These variant repeats or repeat interruptions seem to confer an additional level of epigenetic variability since local DNA methylation is frequently associated with variant CCG repeats independently of the expansion size. The effect of repeat interruptions on DM1 molecular pathogenesis is not investigated enough. Studies on patients indicate their stabilizing effect on DMPK expansions because no congenital cases were described in patients with repeat interruptions, and the age at onset is frequently later than expected. Here, we review the clinical relevance of repeat interruptions in DM1 and genetic and epigenetic characteristics of interrupted DMPK expansions based on patient studies.     

Über die Aufkohlungskinetik von Eisen-Zink-Legierungen

Untersuchung der Aufkohlungskinetik von zwischen 870 und 1107 °C ferritischen und austenitischen Eisen-Zink-Legierungen. Mikrohärtemessungen und Untersuchungen der Kohlenstoffverteilung innerhalb der Aufkohlungsschicht mit der Röntgenmikrosonde. Ermittlung der Wachstumskinetik der Schicht und Erörterung der Ergebnisse im Zusammenhang mit der Diffusion des Kohlenstoffs in den Legierungen.