Surface structures of synthetic diamonds

Diamond crystals of about 2 mm in size were grown by the temperature gradient method using Ni, Ni-Fe alloy and Fe as solvent metals. Several characteristic patterns were seen on the surfaces of the grown diamonds and were found to correspond to the texture of the quenched solvent metals. A vague vein-like pattern appeared when Ni was used. The surface patterns became finer with the increase of the content of Fe in Ni-Fe alloy, and when 70 wt% Fe-30 wt% Ni alloy was used, very fine dendritic patterns were seen on all of the faces of diamond in accordance with the symmetries of the lattice. The patterns are formed by the precipitation of carbon atoms in the space of the dendrites of the solvent metals during the quenching process. Lamellar patterns and etch pits were seen when Fe was used. The patterns are formed when Fe dissolves the diamond surfaces to produce Fe₃C during the quenching process.     

Using bottom surface reflectance to map coastal marine areas: a new application method for Lyzenga's model

Remote sensing is widely used in coastal management. Lyzenga's model has been traditionally used to explain the relationship between bottom surface reflectance and the radiance level measured by satellite. Due to its central assumption, this model lacks accuracy compared with the other radiative transfer models. Nonetheless, it enables, with a single and simple equation, representation of the multiple optical processes taking place in coastal areas. Mapping processes associated with this model may include radiometric correction, a technique previously pointed out as a major driver of mapping accuracy. Radiometric correction is generally based on a depth-invariant index, efficient for clear waters (Jerlov water type I to II) but largely unsuitable when transparency decreases (Jerlov water type II to III). In order to overcome this problem, we developed a new index for radiometric correction, which combines bathymetry data with attenuation coefficients. The improved efficiency of our model with regard to the traditional depth invariant index was demonstrated through two case studies: Funakoshi Bay (Japan; Jerlov water type II) and the Gabes Gulf part located off Mahares (Tunisia; Jerlov water type II to III).     

A rapid-detection method for radioactive cesium in the air

We propose a method of rapidly detecting radioactive cesium floating in the air for the purpose of alerting people active in the ‘difficult-to-return zone’ of Fukushima to changes in airborne radioactivity. With this purpose in mind, we set requirement specifications and created a new system concept. The targeted detection limit was 30 Bq/m³ and the mandatory level of measurement time was 48 min with a target level of 12 min, and these periods are 10 times faster than those of conventional air monitors. The system consists of a conventional gamma-spectrometer with a novel shaped shield. The targeted peak region of the measured energy spectrum is analyzed. After the basic design and confirmation of feasibility, we perform some experiments in the normal environment of Yokohama city and the difficult-to-return zone of Fukushima. Using the results, we conducted the design improvement and performance evaluation. As a result, the system performance is found to satisfy requirements. We thus conclude that this system is a promising candidate for rapid detection of airborne radioactivity in the difficult-to-return zone of Fukushima.     

Ubiquitous Memories: a memory externalization system using physical objects

In this paper we propose an object-triggered human memory augmentation system named “Ubiquitous Memories” that enables a user to directly associate his/her experience data with physical objects by using a “touching” operation. A user conceptually encloses his/her experiences gathered through sense organs into physical objects by simply touching an object. The user can also disclose and re-experience for himself/herself the experiences accumulated in an object by the same operation. We implemented a prototype system composed basically of a radio frequency identification (RFID) device. Physical objects are also attached to RFID tags. We conducted two experiments. The first experiment confirms a succession of the “encoding specificity principle,” which is well known in the research field of psychology, to the Ubiquitous Memories system. The second experiment aims at a clarification of the system’s characteristics by comparing the system with other memory externalization strategies. The results show the Ubiquitous Memories system is effective for supporting memorization and recollection of contextual events.     

Etching of diamond octahedrons at high temperatures and pressure with controlled oxygen partial pressure

Etching of a natural diamond octahedron was carried out at temperatures of 800 to 1400° C and at pressures of 15 and 40 kbar under controlled oxygen partial pressure in the range 10⁻¹⁷ to 10⁴ atm by use of oxygen buffers. Well-defined etch pits of equiangular triangule outline were formed. When the results were plotted based on logP _{O 2} versus 1/T, reversal of the pit orientation clearly occurred on a boundary curve expressed by an equation, logP _{O 2}=−9.0×10⁴/T+63, whereP _{O 2} (atm) andT (K) are oxygen partial pressure and temperature, respectively. Etch pits with the same orientation as an octahedral face were produced in a low temperature and highP _{O 2} region, and those with the opposite orientation, i.e. the same as for natural “trigon”, were produced in the other region.     

Diamond-like carbon thin film with controlled zeta potential for medical material application

With the purpose of applying diamond-like carbon (DLC) thin films as a biocompatible material, we experimented with introducing functional groups such as amino and carboxyl groups to the surface of DLC thin films by plasma surface treatment. From the results, it was found that the contact angle values of the DLC thin films surface were decreased with increasing in the OCO bonded network on the surface. Measurement of the zeta potential when the amounts of the functional groups were varied showed that this successfully varied the zeta potential over the wide range of − 48 mV to + 12 mV. It was found that when carboxyl groups are introduced by O₂ plasma modification of the DLC thin films surface, the zeta potential was lower than that of untreated DLC thin films sample. It was also found that amino groups can be introduced to the DLC thin films surface by NH₃ plasma treatment, the zeta potential was higher than that of untreated DLC thin films sample. This means that zeta potential of the DLC thin films can also be controlled by controlling these two functional groups. Therefore, we have succeeded in developing a multifunctional DLC thin films that does not use polymers and is suitable as a biocompatible material.     

Changes in composition of newly synthesized sphingolipids of HeLa cells during the cell cycle -- suppression of sphingomyelin and higher-glycosphingolipid synthesis and accumulation of ceramide and glucosylceramide in mitotic cells

Sphingolipid biosynthesis in synchronized HeLa cells was studied by pulse labeling with [14C]Ser or [14C]Gal and a simple TLC method. The major HeLa cell sphingolipids are ceramide (Cer), sphingomyelin, glucosylceramide (GlcCer), lactosylceramide (LacCer), globotriaosylceramide (Gb3Cer), N-acetylneuraminosylgangl iotriaosylceramide (GM2) and sialylparagloboside (G[M1-GlcNAc]). The sphingolipid biosynthetic profiles of HeLa cells in the G1, G1/S boundary, S and G2 phases were similar, but significant changes occurred during M phase, when incorporation of radioactivity into sphingomyelin, Gb3Cer and a mixture of GM2 and G(M1-GlcNAc) decreased, and those of Cer and GlcCer increased. These data indicate that transfer of phosphocholine and galactose to Cer and GlcCer, respectively, decreased in mitotic cells, resulting in accumulation of Cer and GlcCer. Analysis of LacCer synthase activity revealed that GlcCer accumulation was not due to reduced activity of this enzyme. The results suggest that Cer and GlcCer accumulation in mitotic cells resulted from suppression of sphingomyelin and LacCer synthesis, probably caused by vesiculation of membranous organelles, such as the endoplasmic reticulum and Golgi apparatus.     

Formation of Cubic Boron Nitride from Rhombohedral Boron Nitride by Explosive Shock Compression

When rhombohedral BN, which has a layer structure with a three-layered stacking sequence, was shock-compressed at 40, 60, and 100 GPa, it was converted to cubic BN. Hexagonal BN was converted to wurtzite-type BN under the same conditions. These results indicate that the transformation proceeds by a diffusionless mechanism in which the stacking sequence of the BN layers in the starting materials is retained during the process.     

An approach for protein to be completely reversible to thermal denaturation even at autoclave temperatures

Reversibility of protein denaturation is a prerequisite forall applications that depend on reliable enzyme catalysis, particularly,for using steam to sterilize enzyme reactors or enzyme sensortips, and for developing protein-based devices that performon–off switching of the protein function such as enzymaticactivity, ligand binding and so on. In this study, we have successfullyconstructed an immobilized protein that retains full enzymaticactivity even after thermal treatments as high as 120°C.The key for the complete reversibility was the development ofa new reaction that allowed a protein to be covalently attachedto a surface through its C-terminus and the protein engineeringapproach that was used to make the protein compatible with thenew attachment chemistry.