DNA Methylation Signatures of Bone Metabolism in Osteoporosis and Osteoarthritis Aging-Related Diseases: An Updated Review

DNA methylation is one of the most studied epigenetic mechanisms that play a pivotal role in regulating gene expression. The epigenetic component is strongly involved in aging-bone diseases, such as osteoporosis and osteoarthritis. Both are complex multi-factorial late-onset disorders that represent a globally widespread health problem, highlighting a crucial point of investigations in many scientific studies. In recent years, new findings on the role of DNA methylation in the pathogenesis of aging-bone diseases have emerged. The aim of this systematic review is to update knowledge in the field of DNA methylation associated with osteoporosis and osteoarthritis, focusing on the specific tissues involved in both pathological conditions.     

Evaluation of damage in composites by using thermoelastic stress analysis: A promising technique to assess the stiffness degradation

The stiffness degradation represents one of the most interesting damage phenomena used for describing the fatigue behaviour of composites. A critical aspect of modelling the damage is represented by the simulation of the whole behaviour of the composite and by the assessment of the actual stiffness for the models validation. In this work, the stiffness degradation of quasi‐isotropic carbon fibre reinforced polymer (CFRP) obtained by automated fibre placement has been assessed by means of thermoelastic stress analysis. The amplitude of temperature signal at the mechanical frequency (thermoelastic signal) was considered as an indicator of material degradation and compared with the data provided by an extensometer. The correlation between thermoelastic and mechanical data allowed to build a new experimental model for evaluating and predicting material stiffness degradation by just using thermoelastic data. The proposed approach seems to be very promising for stiffness degradation assessment of real and complex mechanical components subjected to actual loading conditions.     

ICOSL Stimulation by ICOS-Fc Accelerates Cutaneous Wound Healing In Vivo

Background: ICOS and its ligand ICOSL are immune receptors whose interaction triggers bidirectional signals that modulate the immune response and tissue repair. Aim: The aim of this study was to assess the in vivo effects of ICOSL triggering by ICOS-Fc, a recombinant soluble form of ICOS, on skin wound healing. Methods: The effect of human ICOS-Fc on wound healing was assessed, in vitro, and, in vivo, by skin wound healing assay using ICOS^−/− and ICOSL^−/− knockout (KO) mice and NOD-SCID-IL2R null (NSG) mice. Results: We show that, in wild type mice, treatment with ICOS-Fc improves wound healing, promotes angiogenesis, preceded by upregulation of IL-6 and VEGF expression; increases the number of fibroblasts and T cells, whereas it reduces that of neutrophils; and increases the number of M2 vs. M1 macrophages. Fittingly, ICOS-Fc enhanced M2 macrophage migration, while it hampered that of M1 macrophages. ICOS^−/− and ICOSL^−/− KO, and NSG mice showed delayed wound healing, and treatment with ICOS-Fc improved wound closure in ICOS^−/− and NSG mice. Conclusion: These data show that the ICOS/ICOSL network cooperates in tissue repair, and that triggering of ICOSL by ICOS-Fc improves cutaneous wound healing by increasing angiogenesis and recruitment of reparative macrophages.     

Emerging specializations,competences and firms' proximity in digital industries: The case of London

In order to overcome the limitations of defining industrial specializations in digital industries through SIC codes, this paper suggests measuring the specializations and competences of these industries on the basis of the degree of digital technologies present in the products and services supplied. Metadata from CrunchBase are employed, as proxies of firms' specializations and competences which are defined as the fields of activity in which firms are involved. Applying a network analysis, these specializations and competences are linked to the recognition of emerging digital technologies and the strongest combinations of products and services. We tested the proposed methodology on London, a leading centre for the digital economy.     

Evaluation of log‐of‐power extremum seeking control for wind turbines using large eddy simulations

The extremum seeking control (ESC) algorithm has been proposed to determine operating parameters that maximize power production below rated wind speeds (region II). This is usually done by measuring the turbine's power signal to determine optimal values for parameters of the control law or actuator settings. This paper shows that the standard ESC with power feedback is quite sensitive to variations in mean wind speed, with long convergence time at low wind speeds and aggressive transient response, possibly unstable, at high wind speeds. The paper also evaluates the performance, as measured by the dynamic and steady state response, of the ESC with feedback of the logarithm of the power signal (LP‐ESC). Large eddy simulations (LES) demonstrate that the LP‐ESC, calibrated at a given wind speed, exhibits consistent robust performance at all wind speeds in a typical region II. The LP‐ESC is able to achieve the optimal set‐point within a prescribed settling time, despite variations in the mean wind speed, turbulence, and shear. The LES have been conducted using realistic wind input profiles with shear and turbulence. The ESC and LP‐ESC are implemented in the LES without assuming the availability of analytical gradients.     

Does carbon nanopowder threaten amphibian development?

Lethal and teratogenic potentials of carbon nanoparticles (CNPs) in their amorphous form were investigated by the standardized Frog Embryo Teratogenesis Assay-Xenopus (FETAX), a 96-h in vitro whole-embryo toxicity test based on the amphibian Xenopus laevis. Embryos were acutely exposed to 1, 10, 100 and 500 mg/L CNP suspensions and evaluated for lethality, malformations and growth inhibition. Larvae were processed for histological and ultrastructural analyses to detect the main affected organs, to look for specific lesions at the subcellular level and to image and track CNPs into tissues. Only the highest CNP suspension resulted in being embryolethal for X. laevis larvae, while malformed larva percentages significantly differed from controls starting from 100 mg/L. The stomach and gut were the preferential CNP accumulation sites, on the contrary, the digestive epithelium remained intact. The analyses showed the presence of isolated nanoparticles and/or aggregates in different secondary target organs. CNPs were found in circulating erythrocytes. The research confirms the good tolerance of X. laevis towards pure elemental carbon in its nanoparticulate amorphous form, but highlights the possibility of CNP transfer toward all body areas.     

An exploratory study of the relationship between construction workforce physical strain and task level productivity

The monitoring of construction workforce physical strain can be a valuable management strategy in improving workforce productivity, safety, health, and quality of work. Nevertheless, clear relationships between workforce performance and physical strain have yet to be established. An exploratory investigation of the relationship between task level productivity and physical strain was conducted. Nine participants individually performed a four-hour simulated construction task while a wearable physiological status monitor continuously assessed their physiological condition. Heart rate, relative heart rate, and breathing rate were utilized as predictors of physical strain, and task level–single factor productivity was used as an index of productivity. Numerous regression models were generated using the collected data. This investigation initially unsuccessfully attempted to establish a relationship between physiological condition and productivity at the individual worker level. However, an analysis of the regression models showed that there is a relationship between productivity and either heart rate or relative heart rate at the group level, and that this relationship is parabolic. Breathing rate was proved to not be a significant predictor of productivity. Research results significantly improve understanding of the relationship between work physiology and task productivity. Researchers and practitioners may use the tested monitoring devices, analysis methods, and results to design further applied studies and to improve workforce productivity.     

Control of the J-Aggregation Phenomenon by variation of the N-alkyl-substituents

Cyanine dyes ( 1a–d ) with the 5,5′,6,6′-tetrachloro-1,1′-dialkyl-3,3′-di-(3-carboxypropyl)-benzimidocarbocyanine chromophore differing only in the chain length of their alkyl groups in 1,1′-position have been synthesized, spectroscopically characterized, and compared with 5,5′6,6′-tetrachloro-1,1′-diethyl-3,3′-di-(4-sulfobutyl)-benzimidocarbocyanine( TDBC ). In aqueous solution the dyes form J-aggregates which, depending on the alkyl group chain length, exhibit J-bands differing in spectral positions, bandwidth, and in the number of peaks.     

Traceability of electrical quantities obtained by sampling

The substitution of the traditional analogic measurement and processing techniques with sampling methods is a trend in modern metrology. Many commercial instruments perform this determination by means of their internal firmware. An analysis is necessary when sampling instruments are employed within a laboratory, for the determination of one or more quantities. However, this analysis is possible only under some specific circumstances, where both the characteristics of the samples and the processing algorithms have to be known.The paper will consider the samples as input for the determination of given quantities and the methods to evaluate the uncertainty of the results from the uncertainty of the samples and their correlation. The example is derived by the uncertainty evaluation of the INRIM system for the precision measurement of the power, where the determination of the accuracy has been accomplished both by an almost analytical approach followed by a numerical evaluation.