A Microfabricated Flow Controller for Refrigerant Expansion

A flow controller for refrigerant expansion is reported. Devices are fabricated using a micromolding technique that is developed for thick nickel electrodeposition. The device consists of a short-tube restrictor with an integrated normally open valve, which, when actuated, presents a controllable blockage into the flow passage to obstruct the flow. Fabricated devices are evaluated with compressed air, with up to 22% reduction in mass flow rate at maximum actuation of the restrictor. The devices are also evaluated in an R134a vapor compression system of 1.5-2 kW, with the ability to control mass flow that is found to be greatly influenced by the vapor compression cycle's operational parameters. After the inlet pressure, the level of subcooling proved to be the most important parameter. For a cycle operating between 29 deg C and 4 deg C , saturation temperatures in the condenser and evaporator, respectively, actuation of the device reduced the refrigerant mass flow rate by 3.5% with 0.6 ^oC subcooling and up to 10.8 % with 5 deg C subcooling.     

Dynamics and organization of MAP kinase signal pathways

In the budding yeast, Saccharomyces cerevisiae, four separate but structurally related mitogen-activated protein kinase (MAPK) activation pathways are known. The best understood of these regulates mating. Pheromone binding to receptor informs cells of the proximity of a mating partner and induces differentiation to a mating competent state. The MAPK activation cascade mediating this signal is made up of Ste11 (a MEK kinase [MEKK]), Ste7 (a MAPK/ERK kinase [MEK]), and the redundant MAPK-related Fus3 and Kss1 enzymes. Another MAPK activation pathway is important for cell integrity and regulates cell wall construction. This cascade consists of Bck1 (a MEKK), the redundant Mkk1 and Mkk2 enzymes (MEKs), and Mpk1 (a MAPK). We exploited these two pathways to learn about the coordination and signal transmission fidelity of MAPK activation cascades. Two lines of evidence suggest that the activities of the mating and cell integrity pathways are coordinated during mating differentiation. First, cells deficient in Mpk1 are susceptible to lysis when they make a mating projection in response to pheromone. Second, Mpk1 activation during pheromone induction coincides with projection formation. The mechanism underlying this coordination is still unknown to us. Our working model is that projection formation generates a mobile second messenger for activation of the cell integrity pathway. Analysis of a STE7 mutation gave us some unanticipated but important insights into parameters important for fidelity of signal transmission. The Ste7 variant has a serine to proline substitution at position 368. Ste7-P368 has higher basal activity than the wild-type enzyme but still requires Ste11 for its function. Additionally, the proline substitution enables the variant to transmit the signal from mammalian Raf expressed in yeast. This novel activity suggests that Ste7-P368 is inherently more permissive than Ste7 in its interactions with MEKKs. Yet, Ste7-P368 cross function in the cell integrity pathway occurs only when it is highly overproduced or when Ste5 is missing. This behavior suggests that Ste5, which has been proposed to be a tether for the kinases in the mating pathway, contributes to Ste7 specificity and fidelity of signal transmission.     

Principle Component Analysis in Conjuction with Data Driven Methods for Sediment Load Prediction

This study investigates sediment load prediction and generalization from laboratory scale to field scale using principle component analysis (PCA) in conjunction with data driven methods of artificial neural networks (ANNs) and genetic algorithms (GAs). Five main dimensionless parameters for total load are identified by using PCA. These parameters are used in the input vector of ANN for predicting total sediment loads. In addition, nonlinear equations are constructed, based upon the same identified dimensionless parameters. The optimal values of exponents and constants of the equations are obtained by the GA method. The performance of the so-developed ANN and GA based methods is evaluated using laboratory and field data. Results show that the expert methods (ANN and GA), calibrated with laboratory data, are capable of predicting total sediment load in field, thus showing their transferability. In addition, this study shows that the expert methods are not transferable for suspended load, perhaps due to insufficient laboratory data. Yet, these methods are able to predict suspended load in field, when trained with respective field data.     

Dynamic stability of cracked multi-bay frame structures

The effects of crack depth and crack location on the in-plane static and dynamic stability of cracked multi-bay frame structures subjected to periodic loading have been investigated numerically by using the finite element method. For the rectangular cross-section beam, a crack element is developed by using the principles of fracture mechanics. In addition, the effect of the number of spans and static and dynamic load parameters on static and dynamic stability analysis are also investigated. For buckling and dynamic stability analyses, the results obtained by using the present model are presented in three-dimensional graphical forms and tables.     

Optimization of Ozonation Process for a Composting Leachate-Contaminated Soils Treatment Using Response Surface Method

The ozonation process has become one of the most favorable processes among soil remediation technologies nowadays. Ozone, which has an oxidation potential of 2.07 V and acts as a powerful oxidizer, is capable of degrading organic pollutants of soil in a short period of time without producing any toxic residuals. In this study the capability of ozone, as an ex-situ method of soil treatment, in remediating the leachate-contaminated soil has been investigated. To maximize the removal efficiency of organic content of soil, design of experiments using the response surface method (RSM) and central composite design (CCD) have been employed. To select the range of effective parameters several experiments were performed at laboratory scale. Results showed a range of effective parameters on the ozonation process, including pH, humidity, and initial soil pollution. Present research shows that acid-washed ozone greatly enhanced the removal efficiency. According to the developed model, the maximum removal efficiency using acid-washed ozone was obtained by setting the parameters as pH = 10.8, humidity of 5% and initial organic content of soil to be 7720 mg/kg. Confirmation experiments showed that RSM could be effectively utilized for the optimization of the ozonation process. Analysis of variance also showed that pH was the most significant factor affecting removal efficiency.     

A note on the Consecutive Ones Submatrix problem

A binary matrix has the Consecutive Ones Property (C1P) for columns if there exists a permutation of its rows that leaves the 1's consecutive in every column. The problem of Consecutive Ones Property for a matrix is a special variant of Consecutive Ones Submatrix problem in which a positive integer K is given and we want to know if there exists a submatrix B of A consisting of K columns of A with C1P property. This paper presents an error in the proof of NP-completeness for this problem in the reference cited in text by Garey and Johnson [Computers and Intractability, A Guide to the Theory of NP-Completeness, 1979].     

Comparison Between Using Longitudinal and Shear Waves in Ultrasonic Stress Measurement to Investigate the Effect of Post-Weld Heat-Treatment on Welding Residual Stresses

Fusion welding is a joining process widely used in the industry. However, undesired residual stresses are produced once the welding process is completed. Post-weld heat-treatment (PWHT) is extensively employed in order to relieve the welding residual stresses. In this study, effect of PWHT time and temperature on the residual stresses of a ferritic stainless steel is investigated. Residual stress distributions in eight welded specimens were measured by using an ultrasonic method. Ultrasonic stress measurement is a nondestructive method based on acoustoelasticity law, which correlates mechanical stresses with velocity of an ultrasonic wave propagating within the subject material. The ultrasonic wave employed could be longitudinal or shear wave produced by the longitudinal (normal) or transverse (shear) transducers, respectively. Ultrasonic stress measurements based on longitudinal waves use longitudinal critically refracted (L_CR) waves in this direction, while shear wave methods use an ultrasonic birefringence phenomenon. The results show that the effect of PWHT can be successfully inferred by both longitudinal and shear wave methods, but the former is found to be more sensitive to stress variation. Furthermore, the distribution of subsurface residual stresses is found to be more distinguishable when the L_CR method is employed.     

Printed dual-band base station antenna for GSM/DCS/PCS/UMTS and LTE applications with dual polarization

A new design of the dual-band and dual-polarized base station antennas for supporting the mobile communication systems operating at the GSM/DCS/PCS/UMTS and LTE frequency bands is presented. A wide input impedance matching bandwidth is achieved due to a trident-shaped feeding technique. Two printed dipoles, which are located perpendicularly to each other and fed by stepped-microstrip lines, establish the proposed antenna. In addition, by locating a low-profile cavity-backed structure, as a metal reflector under the antenna, bidirectional radiations of the dipoles are switched to unidirectional radiations with an increase in the gain of the antenna. Measurement results indicate that the proposed antenna is suitable for base station applications at the operating frequencies of 800/900/1800/1900/2300 MHz. The isolation is better than 20 dB, and peak gains of 10.08 and 9.96 dBi are attained at port-1 and port-2, respectively. Furthermore, the HPBWs of the antenna in H-plane is more than 61° for each port. The overall dimension of the antenna is 168 × 168 mm², which is mounted upon a 222 × 222 mm² cavity-backed structure with a depth of 42 mm.     

Engineering Functional Membrane–Membrane Interfaces by InterSpy

Engineering synthetic interfaces between membranes has potential applications in designing non-native cellular communication pathways and creating synthetic tissues. Here, InterSpy is introduced as a synthetic biology tool consisting of a heterodimeric protein engineered to form and maintain membrane–membrane interfaces between apposing synthetic as well as cell membranes through the SpyTag/SpyCatcher interaction. The inclusion of split fluorescent protein fragments in InterSpy allows tracking of the formation of a membrane–membrane interface and reconstitution of functional fluorescent protein in the space between apposing membranes. First, InterSpy is demonstrated by testing split protein designs using a mammalian cell-free expression (CFE) system. By utilizing co-translational helix insertion, cell-free synthesized InterSpy fragments are incorporated into the membrane of liposomes and supported lipid bilayers with the desired topology. Functional reconstitution of split fluorescent protein between the membranes is strictly dependent on SpyTag/SpyCatcher. Finally, InterSpy is demonstrated in mammalian cells by detecting fluorescence reconstitution of split protein at the membrane–membrane interface between two cells each expressing a component of InterSpy. InterSpy demonstrates the power of CFE systems in the functional reconstitution of synthetic membrane interfaces via proximity-inducing proteins. This technology may also prove useful where cell-cell contacts and communication are recreated in a controlled manner using minimal components.