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目的:研究葡萄皮渣提取不溶性膳食纤维对铅中毒模型小鼠体内铅含量的影响。方法:以新鲜的葡萄皮渣为原料,采用发酵法,利用乳酸菌提取不溶性膳食纤维。通过选用KM小鼠进行建模,随机分成对照组、空白组、铅中毒组和膳食纤维-铅中毒组4组。3周后铅中毒组和膳食纤维-铅中毒组造成实验性铅中毒,对照组和膳食纤维-铅中毒组服用膳食纤维至实验结束。分别采集1、3、6、9周后的血液、尿液和粪便,采用微波密闭消解,电感耦合等离子体发射光谱法(ICP-AES法)检测铅浓度。结果:服用膳食纤维的膳食纤维-铅中毒组小鼠血铅含量明显降低,与对照组相比呈现极显著差异(P0.01)。尿铅和粪铅的含量呈现降低趋势。结论:从葡萄皮渣中提取的不溶性膳食纤维有一定的排铅作用,可以有效降低铅中毒模型小鼠体内铅的含量。 相似文献
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Effects of hypoxia on learning-memory function and swimming capability of rat were studied and the ergonomics under hypoxic condition was also evaluated from the biological point of view. Three modes of hypoxia were designed and plots of oxygen concentration versus time for each group in hypoxic environment were produced. Results showed that the effects of hypoxia on learning-memory function and swimming capability were related with the time and strength of hypoxia. It had nothing to do with the individual difference of rat models. 10% O2 long-term intermittent anamorphosis hypoxia could improve the swimming capability of rat model significantly. Stimulating with proper level of hypoxia, carbon dioxide could improve ergonomics in airtight hypoxia environment. Under hypoxia condition, from the ergonomic point of view, 6% O2 is the important threshold and might belong in critical region. 相似文献
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原子光谱法测定金花葵花瓣及种子中微量元素 总被引:1,自引:0,他引:1
金花葵微量元素的测定多采用原子光谱法,但因实验条件、样本预处理方法不一致,各测定方法间的重现性和可比性较差。采用原子吸收分光光度计测定微量元素铜、镁、铁、锰、铅、锌,采用原子荧光分光光度计测定微量元素硒。实验详细研究了微波消解与电热板消解法两种消解方法对含量测定的影响,对金花葵花瓣和种子中的微量元素含量进行了比较。结果表明微波消解法更有利于微量元素含量的测定,精密度与重现性更高。金花葵种子与花瓣相比,种子中的微量元素锌、镁、锰等含量均比花瓣中高,金花葵花瓣及种子中都含有微量元素硒,种子中的微量元素含量达0.063 5 mg/kg,花瓣中的含量为0.027 2 mg/kg。微量元素铅含量没有超标,金葵花种子的药用价值更高。 相似文献
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