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用硅橡胶膜生物反应器系统选育高性能酿酒酵母 总被引:1,自引:1,他引:1
应用硅橡胶膜生物反应器的发酵-渗透汽化耦合功能,进行长期封闭循环发酵,使酵母在这种特定环境下完成适应性自然进化,从而获得优势的突变株。以工业安琪酵母ADY为原始菌株,进行每一轮500h的封闭循环发酵,在发酵终点取菌悬液做平板筛菌,分离出遗传约200代的相对强势的新菌株,再转移新菌株进行同样的发酵和终点筛选实验。如此的发酵-筛选-转移实验共进行了3轮。试验结果表明:ADY遗传超过600代的菌株S33,在500h封闭循环发酵残液配制的培养基条件下,表现出了明显的环境适应性遗传优势,其细胞生长速率、产物乙醇的比生成速率和糖转化率都相对原种菌ADY分别提高了约71.8%、53.6%和52.7%。 相似文献
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The pervaporation of organics from dilute aqueous solution through a novel plate composite silicone rubber membrane was investigated. The measured and derived data indicated that the composite membrane possessed very high permeation flux and stable selectivity for dilute organic aqueous solution. Based upon the well- known resistance-in- series model, diffusive mass transfer behavior in membrane was investigated by calculation from the measured data with different skin layer thickness of membranes. The experimental results showed that the diffusive mass transfer coefficient conformed to Arrhenius correlation with temperature and was independent of the flow status. The diffusivities of the given alcohols in membrane had an order of magnitude 10 m-10·s-1 at a wider range of temperature, which is similar to those reported in literatures. 相似文献
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The pervaporation behavior of fermentation broth was investigated experimentally and compared with those started with ethanol mixtures. Ethanol was produced by Saccharomyces cerevisiae utilizing technical grade glucose and recovered by pervaporation using a composite polydimethylsiloxane (PDMS) membrane prepared in our laboratory. Ethanol concentration in fermentation broth decreased to a relatively low level when pervaporation was coupled with fermentation. The more active cells appeared in the fermentation broth, the better the membrane performance was. 相似文献
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硅橡胶膜生物反应器封闭循环乙醇发酵系统能长时间平稳运行,发酵罐中乙醇浓度、酵母浓度和乙醇产率维持稳定,乙醇产率较传统间歇发酵可提高4.5倍,但系统长期运行的周期受制于酵母细胞的老化。我们目前的实验连续运行记录是500h。系统运行周期结束时,发酵罐中的残液成为需要处理的发酵废弃物,其总量约为传统间歇发酵工艺的1/6,但性态差别很大。对残液进行后处理,有效利用废弃物并实现发酵工艺的废掖零排放,成为该系统的又一主要工艺技术问题。本文对系统运行及工艺技术、残液性态、残液处理工艺路线和设备进行了研究,提出了解决方案。 相似文献
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基于分离效率的卧螺离心机CFD分析 总被引:1,自引:0,他引:1
从分离效率的角度对卧式螺旋卸料离心机转鼓内的流场进行数值分析.通过剖析推料螺旋与转鼓溢流堰的结构特点可知,转鼓内流体在轴向为薄层流动,在径向由于涡流产生向内流动导致颗粒上浮,这两者对离心机分离效率有决定性的影响.结合CFD数值分析和颗粒的Stokes沉降运动关系,提出将薄层流动与上浮临界粒度应用于离心机分离能力计算的理论和方法.文章通过定义沉降粒度和上浮临界粒度,进一步把分离能力计算与分离效率相关联,使该方法更接近离心机分离工艺的实际情况.通过对工业中典型离心机进行实例计算,证明了该方法的可行性和准确性. 相似文献
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