Transglycosylation Activity of Engineered Bifidobacterium Lacto-N-Biosidase Mutants at Donor Subsites for Lacto-N-Tetraose Synthesis

The health benefits of human milk oligosaccharides (HMOs) make them attractive targets as supplements for infant formula milks. However, HMO synthesis is still challenging and only two HMOs have been marketed. Engineering glycoside hydrolases into transglycosylases may provide biocatalytic routes to the synthesis of complex oligosaccharides. Lacto-N-biosidase from Bifidobacterium bifidum (LnbB) is a GH20 enzyme present in the gut microbiota of breast-fed infants that hydrolyzes lacto-N-tetraose (LNT), the core structure of the most abundant type I HMOs. Here we report a mutational study in the donor subsites of the substrate binding cleft with the aim of reducing hydrolytic activity and conferring transglycosylation activity for the synthesis of LNT from p-nitrophenyl β-lacto-N-bioside and lactose. As compared with the wt enzyme with negligible transglycosylation activity, mutants with residual hydrolase activity within 0.05% to 1.6% of the wild-type enzyme result in transglycosylating enzymes with LNT yields in the range of 10–30%. Mutations of Trp394, located in subsite -1 next to the catalytic residues, have a large impact on the transglycosylation/hydrolysis ratio, with W394F being the best mutant as a biocatalyst producing LNT at 32% yield. It is the first reported transglycosylating LnbB enzyme variant, amenable to further engineering for practical enzymatic synthesis of LNT.     

Perceived stress management skill mediates the relationship between optimism and positive mood following radical prostatectomy.

This study evaluated relations among optimism, perceived stress management skills (PSMS), and positive mood in 46 men who had surgical treatment for localized prostate cancer. The authors found that optimism, PSMS, and positive mood scores were positively correlated. Positive mood was unrelated to demographic and disease-related control variables. In a hierarchical regression model controlling for PSMS, the relationship between optimism and positive mood became nonsignificant, whereas PSMS remained a correlate of positive mood. Results suggest that the relationship between optimism and positive mood may be mediated by belief in being able to use stress management techniques effectively. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Defensiveness, trait anxiety, and Epstein-Barr viral capsid antigen antibody titers in healthy college students.

Investigated the relationship of individual differences in repressive coping styles with differences in antibody titer to Epstein-Barr (EB) viral capsid antigen in a normal, healthy college population made up of people previously exposed to EB. Each of 54 1st-yr undergraduates completed a battery of physical-status questions and items pertaining to potential behavioral immunomodulatory confounds, along with the Taylor Manifest Anxiety Scale and the Marlowe-Crowne Social Desirability Scale. Ss reporting high and middle levels of anxiety had higher antibody titers to EB, suggesting poorer immune control over the latent virus, as compared with the low-anxious group. Similarly, high-defensive Ss had higher antibody titers than their low-defensive counterparts, and neither group differed from the middle group. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Mobile context-based framework for threat monitoring in urban environment with social threat monitor

Engaging users in threat reporting is important in order to improve threat monitoring in urban environments. Today, mobile applications are mostly used to provide basic reporting interfaces. With a rapid evolution of mobile devices, the idea of context awareness has gained a remarkable popularity in recent years. Modern smartphones and tablets are equipped with a variety of sensors including accelerometers, gyroscopes, pressure gauges, light and GPS sensors. Additionally, the devices become computationally powerful which allows for real-time processing of data gathered by their sensors. Universal access to the Internet via WiFi hot-spots and GSM network makes mobile devices perfect platforms for ubiquitous computing. Although there exist numerous frameworks for context-aware systems, they are usually dedicated to static, centralized, client-server architectures. There is still space for research in the field of context modeling and reasoning for mobile devices. In this paper, we propose a lightweight context-aware framework for mobile devices that uses data gathered by mobile device sensors and performs on-line reasoning about possible threats based on the information provided by the Social Threat Monitor system developed in the INDECT project.     

Indecomposability of free algebras in some subvarieties of residuated lattices and their bounded subreducts

In this paper, we show that free algebras in the variety of residuated lattices and some of its subvarieties are directly indecomposable and show, as a consequence, the direct indecomposability of free algebras for some classes of their bounded implicative subreducts.     

Hybrid phenol biosensor based on modified phenoloxidase electrode

Electrolytic deposition has been widely used to immobilize biomacromolecules, and it is always the most important factor to preserve or even increase an activity of the immobilized protein. We report here simple and rather universal method for the highly efficient immobilization of laccase for amperometric biosensing. Laccase from Cerrena unicolor has been successfully immobilized (electrolytic deposition) on the surface of thin, ordered polythiophene films (3-methylthiophene/3-thiopheneacetic acid/N-heptyl-3,6-bis(2-thiophene)carbazole). Two different compounds capable of mediating laccase-catalyzed reactions have been tested by cyclic voltammetry. They exhibited quasi-reversible electrodic behaviour with formal redox potentials ranging from 68 and 918 mV (E⁰vs. SCE). The immersion of the laccase-coated electrode in solution with substrate generated large catalytic currents easily recorded by cyclic voltammetry at low potential scan rates. Considering the fact, that immobilization strategy showed high efficiency, obtained results suggest that method for phenoloxidase immobilization has a great potential of enabling high throughput fabrication of bioelectronics’ devices.     

Quantitative analysis in a heuristic evaluation experiment

In usability context of interactive systems the heuristic evaluation method is widespread. In most applications the results tend to be qualitative, describing such aspects that require some improvement for the benefit of usability. However, these qualitative results do not allow us to determine how usable it is or how it becomes an interactive system. Hence the need for quantitative results may also be very necessary in order to determine the effort that would be needed to get a sufficiently usable system.This article describes, following the idea of the UsabAIPO Project, a new experiment to obtain quantitative results after a heuristic evaluation. This new experimentation has required some variation on the original idea, working with a set of different heuristic categories, while considering the use of the score depending on severity and frequency parameters.     

Wherein is Human Cognition Systematic?

The ??systematicity argument?? has been used to argue for a classical cognitive architecture (Fodor in The Language of Thought. Harvester Press, London, 1975, Why there still has to be a language of thought? In Psychosemantics, appendix. MIT Press, Cambridge, pp 135?C154, 1987; Fodor and Pylyshyn in Cognition 28:3?C71, 1988; Aizawa in The systematicity arguments. Kluwer Academic Press, Dordrecht, 2003). From the premises that cognition is systematic and that the best/only explanation of systematicity is compositional structure, it concludes that cognition is to be explained in terms of symbols (in a language of thought) and formal rules. The debate, with connectionism, has mostly centered on the second premise-whether an explanation of systematicity requires compositional structure, which neural networks do not to exhibit (for example, Hadley and Hayward, in Minds and Machines, 7:1?C37). In this paper, I will take issue with the first premise. Several arguments will be deployed that show that cognition is not systematic in general; that, in fact, systematicity seems to be related to language. I will argue that it is just verbal minds that are systematic, and they are so because of the structuring role of language in cognition. A dual-process theory of cognition will be defended as the best explanation of the facts.     

A Stress and Coping Model of Medication Adherence and Viral Load in HIV-Positive Men and Women on Highly Active Antiretroviral Therapy (HAART).

The authors tested a structural model that incorporated age, time since diagnosis, social support, coping, and negative mood as predictors of medication adherence and HIV viral load on 188 men and 134 women on highly active antiretroviral therapy (HAART). The authors used psychosocial latent factors formed from baseline measures to predict latent factors of adherence, as assessed by electronic monitoring and self-report, and viral load defined by indicators assessed over a 15-month period. Results from the model indicate that greater negative mood and lower social support are related to greater use of avoidance-oriented coping strategies. Use of these coping strategies by patients on HAART is related to poorer medication adherence and, subsequently, higher viral load. This model advances researchers' understanding of the contribution of psychosocial variables in predicting treatment adherence and disease progression in HIV-positive men and women. (PsycINFO Database Record (c) 2010 APA, all rights reserved)