维珍果 品质创造效益

<正>"维珍果",富含维生素的珍(榛)果,是土耳其榛子在中国的注册商标名称。土耳其维珍果(中国)推广集团是由土耳其榛子推广集团为在中国开展榛子推广活动而与剑平国际集团合作成立的,旨在全国范围内推广土耳其榛子的机构。该机构成立于2001年,并于2003年在位于北京怀柔的雁栖工业开发区建立了土耳其维珍果示范中心,同时依托此示范中心成立了北京芬帝食品有限公司。     

Escherichia coli, enterococci, and Bacteroides thetaiotaomicron qPCR signals through wastewater and septage treatment

Fecal indicators such as Escherichia coli and enterococci are used as regulatory tools to monitor water with 24 h cultivation techniques for possible input of sewage or feces and presence of potential enteric pathogens yet their source (human or animal) cannot be determined with routine methods. This critical uncertainty has furthered water pollution science toward new molecular approaches. Members of Bacteroides genus, such as Bacteroides thetaiotaomicron are found to have features that allow their use as alternative fecal indicators and for Microbial Source Tracking (MST). The overall aim of this study was to evaluate the concentration and fate of B. thetaiotaomicron, throughout a wastewater treatment facility and septage treatment facility. A large number of samples were collected and tested for E. coli and enterococci by both cultivation and qPCR assays. B. thetaiotaomicron qPCR equivalent cells (mean: 1.8 × 10⁷/100 mL) were present in significantly higher concentrations than E. coli or enterococci in raw sewage and at the same levels in raw septage. The removal of B. thetaiotaomicron target qPCR signals was similar to E. coli and enterococci DNA during the treatment of these wastes and ranged from 3 to 5 log₁₀ for wastewater and was 7 log₁₀ for the septage. A significant correlation was found between B. thetaiotaomicron marker and each of the conventional indicators throughout the waste treatment process for both raw sewage and septage. A greater variability was found with enterococci when compared to E. coli, and CFU and equivalent cells could be contrasted by various treatment processes to examine removal and inactivation via septage and wastewater treatment. These results are compared and contrasted with other qPCR studies and other targets in wastewater samples providing a view of DNA targets in such environments.     

Nanogold plasmon resonance-based glucose sensing. 2. Wavelength-ratiometric resonance light scattering

Gold colloids are well known to display strong plasmon absorption bands due to electron oscillations induced by the incident light. When the colloids are in proximity, the plasmon absorption bands are often perturbed. This has enabled us recently to successfully develop a glucose sensing platform based on the disassociation of dextran-coated gold colloids, cross-linked with Con A, by glucose. However, a much more useful and simpler property of gold colloids, which has been ill explored with regard to sensing, is their ability to efficiently scatter excitation light. We have found that our nanogold sensing aggregates are indeed efficient light scatters around the nanogold plasmon absorption band. By measuring the ratio of scattered light intensities at two different arbitrary wavelengths, 560 and 680 nm, glucose concentrations can be readily determined from a few millimolar up to approximately 60 mM, using a simple white light LED and detection system. Further, by measuring the ratio of the scattered intensities, this sensing approach is independent of the total sensing aggregate concentration and the excitation and detection instrumentation fluctuations or drifts. This simplistic and low-cost approach to glucose sensing, coupled with the sensing aggreagates' ability to scatter red light, suggests the potential use of these aggregates for use in physiological transdermal glucose monitoring, either for implantable skin sensors or glucose sensing tattoos (discussed later).     

利用胶原水解物制备枯草芽孢杆菌ATCC6633角蛋白酶的研究

本研究中,我们在碱性条件下采用商业蛋白酶水解制革厂的铬鞣革屑。随后进行了一些化学的分析并且测定处理铬鞣革屑后得到的胶原水解物的氨基酸组成。胶原水解物可以作为碳和氮的来源生产枯草芽孢杆菌ATCC6633角蛋白酶。在pH7．0和pH10．0,搅拌速率200rpm时,采用不同浓度的胶原水解液（1％～5％w／v）进行培养发酵。角蛋白酶的最佳工艺条件是：含有1％水解物（w／v）在pH7．0条件下发酵36小时以及含有3％水解物（w／v）在pH10．0条件下发酵24小时。     

Detection of the frequency, antimicrobial susceptibility, and genotypic discrimination of Aeromonas strains isolated from municipally treated tap water samples by cultivation and AP-PCR

The frequency, antibiotic susceptibility, and genotypic discrimination of Aeromonas strains isolated from municipally treated drinking tap water distribution systems were investigated in this study. We have analyzed 148 tap water samples collected from 8 different locations by bacterial cultivation and arbitrarily primed polymerase chain reaction (AP-PCR). Gram negative, hemolytic, oxidase (+) and catalase (+) bacterial colonies were applied to the study. Identification of bacterial colonies was done by conventional biochemical method and API ID 20E panel (BioMerieux-France). Molecular epidemiological discrimination of the isolates was done by AP-PCR. Aeromonas spp. was detected in 6 of 148 (4%) tap water samples from 8 different locations. Five isolates were identified as Aeromonas hydrophila and one isolate was identified as Vibrio fluvialis by conventional biochemical method. These data were also confirmed by API 20E panel. One of 6 isolates was resistant to gentamicin, 2 of 6 isolates were resistant to trimethoprim/sulfamethoxazole, 4 of 6 isolates were resistant to ampicillin and ampicillin-sulbactam and all of 6 isolates were resistant to cephalothin. All isolates were found to be susceptible to amikacin, aztreonam, ceftazidime, ceftriaxone, ciprofloxacin. All 6 strains of Aeromonas were discriminated by AP-PCR and were determined that all isolates were from different genotypic sources. Although the frequency of the isolates was under the standard limits, the results indicate that hemolytic A. hydrophila are present in municipally treated tap water samples in Mersin City. While all strains were genotypically distinct, all of them were resistant to first generation beta lactam antibiotics tested in this study.     

Rapid crystallization of L-arginine acetate on engineered surfaces using metal-assisted and microwave-accelerated evaporative crystallization()

We report the application of our newly described crystallization technique, which employs silver island films (SIFs) and microwave heating, to rapid crystallization of L-arginine acetate (LAA). Using our technique, LAA crystals (~ 1.2 mm in length) were grown from a 20 μl solution in 1 min on surface functionalized SIFs. In control experiments (glass slides and at room temperature) the growth of LAA crystals (0.1-0.3 mm) took ~ 55 min.     

第十五届土耳其电子显微学会议

第十五届土耳其电子显微学会议(15th National Electron Microscopy Congress)将 在2001年9月18日—21日在土耳其国Kusadasi举行。 　　 地点： Pine Bay Holiday Resort in Kusadasi Congress secreteriat: 　　Serenas Turizm 　　Cinnah Cad. 2817 　　Cankaya-Ankara 　　Turkey Tel: +90 312 467 6991 Fax: +90 312 467 6072 E-mail: serenas@serenas.com.tr     

Spatial and temporal control of microwave triggered chemiluminescence: a protein detection platform

We have combined the principles of microwave circuitry and antenna design and our recent work in microwave-triggered metal-enhanced chemiluminescence to now "trigger" chemically and enzyme-catalyzed chemiluminescent reactions with spatial and temporal control. With this technology platform, we achieve spatial and temporal control of enzyme and chemically catalyzed chemiluminescence reactions to achieve more than 500-fold increases in "on-demand" photon flux from chemically catalyzed chemiluminescent reactions. We also report a 6-fold increase in photon flux from HRP-catalyzed assays on disposable coverslips functionalized with HRP and placed proximal to the substrates modified with thin-film aluminum triangle disjointed "bow-tie" structures. In addition, we demonstrate the applicability of this technology to develop multiplexed or high-throughput chemiluminescent assays. We also demonstrate the clinical and biological relevance of this technology platform by affixing aluminum structures in proximity to HRP protein immobilized on nitrocellulose to improve the sensitivity for this model Western blot scheme by 50-fold. We believe analytical applications that rely on enzyme-catalyzed chemiluminescence, such as immunoassays, may greatly benefit from this new platform technology.     

Biological denitrification of drinking water in a slow sand filter

Biological removal of nitrate from drinking water was studied in a slow sand filter. Optimum carbon to nitrogen ratio (C/N) was found to be 1.8 when using acetic acid in batch tests. The filtration rates impact on NO(3)-N removal through the sand filter was assessed for 22.6 mgNO(3)-N/l concentrations while keeping C/N ratio as 1.8 for acetic acid. The filtration rates varied from 0.015, 0.02, 0.03, 0.04, 0.05, and 0.06 m/h, respectively, corresponding to an overall average NO(3)-N removal efficiency of 94%. Although increasing filtration rates decreased NO(3)-N removal, effluent NO(3)-N concentrations at the effluent port were lower than the limit value. The slow sand filter process was unable to provide NO(3)-N removal rate more than 27.1 gN/(m(2)day) (0.05 m/h flow rate). The NO(3)-N removal efficiency slightly dropped from 99% to 94% when the loading rate increased from 27.1 to 32.5 g/(m(2)day), but the effluent water contained higher concentration of NO(2)-N than the standard value.