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1.
Paolo Feraboli Attilio Masini Luigi Taraborrelli Andrea Pivetti 《Composite Structures》2007,78(4):495-506
When modern saloon cars are re-engineered as convertibles (or roadsters) it is typical for them to lose 50% or more of the body’s torsional rigidity. Consequently the vehicles rarely handle quite as crisply, nor do they ride as well as the coupes from which they derived. This paper highlights the fundamental contributions of advanced composites in achieving the desired value of handling of the Murcièlago Roadster without penalizing the overall weight of the vehicle. To compensate for the absence of the roof structure, the vehicle was strongly redesigned by introducing new structural members and reinforcing existing critical components. A new all-carbon/epoxy composite sub-frame, which spans the entire engine bay, is comprised of elliptical tubular members, and it is the first of its kind in a production vehicle. Engineering of the sub-frame, from preliminary design to manufacturing decisions, is the focus of this paper, and is achieved through a Building Block approach that sets the program within the Integrated Product Development (IPD) strategy that the Advanced Composites Division of Automobili Lamborghini S.p.A. employs for its technology demonstrators. The strategy consists in a concurrent analytical and experimental development of the product, from the initial conceptual design and coupon testing, through the stages of element and subcomponent engineering, to final component manufacturing. 相似文献
2.
3.
CT Migita KM Matera M Ikeda-Saito JS Olson H Fujii T Yoshimura H Zhou T Yoshida 《Canadian Metallurgical Quarterly》1998,273(2):945-949
The O2 and CO reactions with the heme, alpha-hydroxyheme, and verdoheme complexes of heme oxygenase have been studied. The heme complexes of heme oxygenase isoforms-1 and -2 have similar O2 and CO binding properties. The O2 affinities are very high, KO2 = 30-80 microM-1, which is 30-90-fold greater than those of mammalian myoglobins. The O2 association rate constants are similar to those for myoglobins (kO2' = 7-20 microM-1 s-1), whereas the O2 dissociation rates are remarkably slow (kO2 = 0.25 s-1), implying the presence of very favorable interactions between bound O2 and protein residues in the heme pocket. The CO affinities estimated for both isoforms are only 1-6-fold higher than the corresponding O2 affinities. Thus, heme oxygenase discriminates much more strongly against CO binding than either myoglobin or hemoglobin. The CO binding reactions with the ferrous alpha-hydroxyheme complex are similar to those of the protoheme complex, and hydroxylation at the alpha-meso position does not appear to affect the reactivity of the iron atom. In contrast, the CO affinities of the verdoheme complexes are >10,000 times weaker than those of the heme complexes because of a 100-fold slower association rate constant (kCO' approximately 0. 004 microM-1 s-1) and a 300-fold greater dissociation rate constant (kCO approximately 3 s-1) compared with the corresponding rate constants of the protoheme and alpha-hydroxyheme complexes. The positive charge on the verdoporphyrin ring causes a large decrease in reactivity of the iron. 相似文献
4.
Ceri S. Daniel F. Matera M. Raffio A. 《Knowledge and Data Engineering, IEEE Transactions on》2009,21(6):894-909
While business process definition is becoming more and more popular as an instrument for describing human activities, there is a growing need for software tools supporting business process abstractions to help users organize and monitor their desktop work. Tools are most effective when they embed some knowledge about the process, e.g., in terms of the typical activities required by the process, so that users can execute the activities without having to define them. Tools must be lightweight and flexible, so as to enable users to create or change the process as soon as there is a new need. In this article, we first describe an application-independent approach to flexible process support by discussing the abstractions required for modeling, creating, enacting, and modifying flexible processes. Then, we show our approach at work in the context of project-centered learning. In this application, learners are challenged to perform concrete tasks in order to master specific subjects; in doing so, they have to conduct significant projects and cope with realistic (or even real-life) working conditions and scenarios. Often, students are geographically dispersed or under severe timing constraints, because these activities intertwine with their normal university activity. As a result, they need communication technology in order to interact and workflow technology in order to organize their work. The developed platform provides a comprehensible, e-learning-specific set of activities and process templates, which can be combined through a simple Web interface into project-centered collaboration processes. We discuss how the general paradigm of flexible processes was adapted to the learning concept, implemented, and experienced by students. 相似文献
5.
Coiled bodies (CBs) are nuclear organelles whose structures appear to be highly conserved in evolution. In rapidly cycling cells, they are typically located in the nucleoplasm but are often found in contact with the nucleolus. The CBs in human cells contain a unique protein, called p80-coilin. Studies on amphibian oocyte nuclei have revealed a protein within the "sphere" organelle that shares significant structural similarity to p80-coilin. Spheres and CBs are also highly enriched in small nuclear ribonucleoproteins and other RNA-processing components. We present evidence that, like spheres, CBs contain U7 small nuclear RNA (snRNA) and associate with specific chromosomal loci. Using biotinylated 2'-O-methyl oligonucleotides complementary to the 5' end of U7 snRNA and fluorescence in situ hybridization, we show that U7 is distributed throughout the nucleoplasm, excluding nucleoli, and is concentrated in CBs. Interestingly, we found that CBs often associate with subsets of the histone, U1, and U2 snRNA gene loci in interphase HeLa-ATCC and HEp-2 monolayer cells. However, in a strain of suspension-grown HeLa cells, called HeLa-JS1000, we found a much lower rate of association between CBs and snRNA genes. Possible roles for CBs in the metabolism of these various histone and snRNAs are discussed. 相似文献
6.
Carlo Matera Pablo Calv Vernica Casad-Anguera Rosalba Sortino Alexandre M. J. Gomila Estefanía Moreno Thomas Gener Cristina Delgado-Sallent Pau Nebot Davide Costazza Sara Conde-Berriozabal Merc Masana Jordi Hernando Vicent Casad M. Victoria Puig Pau Gorostiza 《International journal of molecular sciences》2022,23(17)
Understanding the dopaminergic system is a priority in neurobiology and neuropharmacology. Dopamine receptors are involved in the modulation of fundamental physiological functions, and dysregulation of dopaminergic transmission is associated with major neurological disorders. However, the available tools to dissect the endogenous dopaminergic circuits have limited specificity, reversibility, resolution, or require genetic manipulation. Here, we introduce azodopa, a novel photoswitchable ligand that enables reversible spatiotemporal control of dopaminergic transmission. We demonstrate that azodopa activates D1-like receptors in vitro in a light-dependent manner. Moreover, it enables reversibly photocontrolling zebrafish motility on a timescale of seconds and allows separating the retinal component of dopaminergic neurotransmission. Azodopa increases the overall neural activity in the cortex of anesthetized mice and displays illumination-dependent activity in individual cells. Azodopa is the first photoswitchable dopamine agonist with demonstrated efficacy in wild-type animals and opens the way to remotely controlling dopaminergic neurotransmission for fundamental and therapeutic purposes. 相似文献
7.
Verônica M. F. Frade Attilio Converti Saleh Al Arni Milena F. Silva Mauri S. A. Palma 《化学工程与技术》2017,40(4):663-669
Enrofloxacin is a synthetic second‐generation fluoroquinolone used as an antimicrobial agent exclusively in veterinary medicine. To simulate the treatment of wastewater contaminated by enrofloxacin, four‐day long fed‐batch runs were carried out according to the Fenton process with an enrofloxacin solution as model, to which hydrogen peroxide and ferrous ion were added twice a day. The residual enrofloxacin concentration was practically coincident to that detected at the end of the batch tests. Hydrogen peroxide was almost completely consumed after each feeding period, while the total organic carbon (TOC) concentration decreased gradually within three days, corresponding to a reduction > 58 %. From the third day on, the TOC falling rate was quite low. A yellow sludge settled due to the precipitation of both Fe(OH)3 and a complex formed by ferric ion with adsorbed enrofloxacin and/or its oxidation products. 相似文献
8.
Elisabetta Finocchio Alessandra Lodi Carlo Solisio Attilio Converti 《Chemical engineering journal (Lausanne, Switzerland : 1996)》2010,156(2):264-269
Biosorption of heavy metals is an interesting approach to treat industrial wastewaters by an environmentally friendly system. Spirulina platensis biomass, an effective biosorbent for cations, cannot be used to adsorb chromate due to its negatively charged surface close to neutral conditions; therefore, methylation of biomass was performed to increase its adsorption capacity under these conditions. Batch adsorption tests carried out varying both Cr(VI) and methylated biomass concentrations showed that 2–4 g l?1 of biosorbent were able to remove Cr(VI) with efficiency ≥80%, while higher Cr(VI) levels (43–50 mg l?1) showed low removal efficiency. The model of Langmuir was shown to describe the adsorption phenomenon better than the Freundlich one. The values of the overall adsorption capacity of methylated biomass suggested that increased biosorbent availability does not necessarily correspond to larger amount of adsorbed metal. FT-IR spectra of dried and methylated biomass of S. platensis allowed us monitoring the efficiency of the methylation process through the analysis of CH and COO? vibrational stretching modes, taken as diagnostic of this process. 相似文献
9.
Quality of service control in Ethernet passive optical networks based on virtual private LAN service technique 总被引:1,自引:0,他引:1
A technique is proposed that allows achievement of logical point-to-multipoint paths in gigabit Ethernet (GbE) networks with access based on passive optical networks (PONs), guaranteeing upstream/downstream bandwidth and quality of service also in conditions of traffic congestion. Such a method is based on the combination of the virtual private LAN service (VPLS), implemented in the core network, and the VLAN tagging forwarding process used in the edge-PON segment. 相似文献
10.
Inulin was used as a prebiotic to improve quality of skim milk fermented by pure cultures of Lactobacillus acidophilus, Lactobacillus rhamnosus, Lactobacillus bulgaricus and Bifidobacterium lactis, binary co-cultures with Streptococcus thermophilus, or a cocktail containing all them. Inulin supplementation to pure cultures lowered the generation time, with particular concern to S. thermophilus and L. acidophilus. The generation time of all micro-organisms decreased in the following order: mono-cultures, co-cultures, cocktail. It was demonstrated a synergism between S. thermophilus and the other strains and a bifidogenic effect of inulin. Enumerations of L. rhamnosus in cocktail markedly decreased compared to co-cultures likely because of greater competition for the same substrates. The results of this work highlight the industrial potential of the cocktail, mainly in terms of fermentation acceleration. 相似文献