全文获取类型
收费全文 | 4860篇 |
免费 | 13篇 |
国内免费 | 3篇 |
专业分类
电工技术 | 19篇 |
综合类 | 3篇 |
化学工业 | 246篇 |
金属工艺 | 25篇 |
机械仪表 | 41篇 |
建筑科学 | 62篇 |
矿业工程 | 10篇 |
能源动力 | 30篇 |
轻工业 | 140篇 |
水利工程 | 9篇 |
石油天然气 | 6篇 |
无线电 | 131篇 |
一般工业技术 | 216篇 |
冶金工业 | 3757篇 |
原子能技术 | 10篇 |
自动化技术 | 171篇 |
出版年
2021年 | 13篇 |
2020年 | 9篇 |
2019年 | 12篇 |
2018年 | 13篇 |
2016年 | 15篇 |
2015年 | 9篇 |
2014年 | 22篇 |
2013年 | 46篇 |
2012年 | 34篇 |
2011年 | 44篇 |
2010年 | 29篇 |
2009年 | 30篇 |
2008年 | 43篇 |
2007年 | 54篇 |
2006年 | 28篇 |
2005年 | 39篇 |
2004年 | 27篇 |
2003年 | 42篇 |
2002年 | 28篇 |
2001年 | 27篇 |
2000年 | 30篇 |
1999年 | 158篇 |
1998年 | 1278篇 |
1997年 | 686篇 |
1996年 | 457篇 |
1995年 | 235篇 |
1994年 | 223篇 |
1993年 | 211篇 |
1992年 | 57篇 |
1991年 | 50篇 |
1990年 | 56篇 |
1989年 | 55篇 |
1988年 | 57篇 |
1987年 | 51篇 |
1986年 | 46篇 |
1985年 | 72篇 |
1984年 | 18篇 |
1983年 | 20篇 |
1982年 | 31篇 |
1981年 | 27篇 |
1980年 | 33篇 |
1979年 | 19篇 |
1978年 | 31篇 |
1977年 | 78篇 |
1976年 | 162篇 |
1975年 | 18篇 |
1974年 | 18篇 |
1973年 | 13篇 |
1972年 | 17篇 |
1944年 | 9篇 |
排序方式: 共有4876条查询结果,搜索用时 0 毫秒
1.
2.
Phospholamban (PLN) was expressed in Escherichia coli as a protein fusion with glutathione S-transferase (GST). GST-PLN was mostly present in the insoluble protein fraction and accounted for approximately 50% of total insoluble protein. Attempts to suppress inclusion body formation or to use GST as an affinity-purification tag failed. A successful purification method is based on preparative SDS/PAGE and electrodialysis. From 1 g cells we typically purified 13.5 mg fusion protein with a PLN content of 2.8 mg. We genetically inserted an enterokinase (EK) protease site just in front of the PLN sequence and demonstrated the proteolytical liberation of PLN from the carrier protein. The approach described represents a substantial advancement in PLN expression and purification. 相似文献
3.
4.
Antibiotic treatment options for Burkholderia cepacia infection are limited because of high intrinsic resistance. The problem is complicated by development of cross-resistance between antibiotics of different classes. We isolated antibiotic-resistant mutants by stepwise exposure to chloramphenicol (Chlor) and to trimethoprim/sulphamethoxazole (T/S) for four B. cepacia strains: ATCC13945, Per (clinical isolate), Cas and D4 (environmental isolates). Chlor(r) mutants did not produce chloramphenicol acetyl-transferase. Cross-resistance, defined as greater than four-fold increase in MIC by microtitre dilution method, was consistently seen in both types of mutants. For chloramphenicol-resistant (Chlor[r]) and trimethoprim/sulphamethoxazole-resistant (Tr/Sr) mutants of B. cepacia ATCC13945 and Cas, no MIC change was seen for piperacillin, ceftazidime, rifampicin, gentamicin, tobramycin, polymyxin B or azithromycin. B. cepacia-Per and -D4 mutants showed cross-resistance to ceftazidime and to piperacillin. Comparison of outer membrane protein (OMP) profiles of B. cepacia and their mutants by SDS-PAGE revealed Tr/Sr) mutants to be deficient in a major OMP (molecular weight 39-47 kDa). Tr/Sr mutants also expressed additional OMPs not found in wild type strains at 75-77 kDa for B. cepacia-ATCC13945 and -Cas, and 20-21 kDa in B. cepacia-D4 and -Per. No OMP changes occurred in Chlor(r) mutants. Lipopolysaccharide (LPS) profiles of each type of mutant showed new high and low molecular weight LPS bands. Cross-resistance seems to be mediated by alterations in porin and LPS for Tr/Sr mutants, but only by LPS in Chlor(r) mutants. 相似文献
5.
DJ Conway MJ Holland RL Bailey AE Campbell OS Mahdi R Jennings E Mbena DC Mabey 《Canadian Metallurgical Quarterly》1997,65(3):1003-1006
Tumor necrosis factor alpha (TNF-alpha) may play a central role in the disease pathogenesis which occurs as a consequence of chlamydial infection. To investigate the importance of TNF-alpha gene promoter polymorphisms and TNF-alpha levels in tear fluid in scarring trachoma, a large matched-pair case-control study was performed in The Gambia. The -308A allele was present in a higher proportion of patients (28.4%) than controls (18.4%), with an increasing association for homozygotes (chi2 for trend, P = 0.032; allele frequency, 0.163 in patients and 0.099 in controls; chi2, P = 0.025). For the -238A allele, the association was similar but not significant. The disease association was highly significant when the number of either -308A or -238A sites in an individual was considered (P = 0.003). TNF-alpha promoter alleles are tightly linked to some HLA class I and II alleles, but multivariate analysis confirmed that the disease associations were independent of HLA, although a class I allele, A*6802, is also associated with disease. TNF-alpha was more frequently detected in tear samples from patients (27.6%) than from controls (15.9%), increasingly so for higher levels of detectable TNF-alpha (P = 0.015). Among patients, detectable TNF-alpha in tears was highly associated with the presence of ocular chlamydial infection (P < 0.001). The results indicate that TNF-alpha plays a major role in the tissue damage and scarring which occurs as a consequence of Chlamydia trachomatis infection. 相似文献
6.
Vertical phoria (vertical vergence in the absence of binocular feedback) can be trained to vary with non-visual cues such as vertical conjugate eye position, horizontal conjugate eye position and horizontal vergence. These prior studies demonstrated a low-level association or coupling between vertical vergence and several oculomotor cues. As a test of the potential independence of multiple eye-position cues for vertical vergence, context-specific adaptation experiments were conducted in three orthogonal adapting planes (midsagittal, frontoparallel, and transverse). Four vertical disparities in each of these planes were associated with various combinations of two specific components of eye position. Vertical disparities in the plane were associated with horizontal vergence and vertical conjugate eye position; vertical disparities in the frontoparallel plane were associated with horizontal and vertical conjugate eye position; and vertical disparities in the transverse plane were associated with horizontal vergence and horizontal conjugate eye position. The results demonstrate that vertical vergence can be adapted to respond to specific combinations of two different sources of eye-position information. The results are modeled with an association matrix whose inputs are two classes of eye position and whose weighted output is vertical vergence. 相似文献
7.
This paper addresses the existence of loop gain-phase shaping (LGPS) solutions for the design of robust digital control systems for SISO, minimum-phase, continuous-time processes with parametric uncertainty. We develop the frequency response properties of LGPS for discrete-time systems using the Δ-transform, a transform method that applies to both continuous-time and discrete-time systems. A theorem is presented which demonstrates that for reasonable specifications there always exists a sampling period such that the robust digital control problem has a solution. Finally, we offer a procedure for estimating the maximum feasible sampling period for LGPS solutions to robust digital control problems. 相似文献
8.
9.
10.