Capillary Filling in Nanochannels—Modeling,Fabrication, and Experiments

While capillary filling in channels of micrometers scale is experimentally verified to obey Washburn's law well, the speed of capillary filling in nanochannels is noticeably lower than described by Washburn's formula. This article reports the theoretical and experimental results on capillary filling in open-end and closed-end nanochannels. Nanochannels of 45 nm and 80 nm depth, 10 μm width, were etched in silicon and bonded to a glass cover. Experiments on filling of non-electrolytic liquid in silicon nanochannels were carried out. The filling processes were observed and recorded. To estimate the influence of electrokinetics, a mathematical model to calculate the electroviscous effect was established. This model shows that the contribution of the electroviscous effect in the reduction of filling speed is small. This result also agrees well with previous theoretical work on the electroviscous effect. That means that besides the electroviscous effect, there are other phenomena that contribute to the reduction of capillary filling speed in a nanochannel, such as air bubbles formation. Experimental investigation of capillary filling in open-end and closed-end nanochannels with different lengths was performed. The filling processes of ethanol and isopropanol and the behavior of the trapped air were recorded and evaluated. Analytical models based on the continuum assumption were used to evaluate the experimental data. We observed that the filling process consists of two stages. At the initial stage, experimental data agree well with the theoretical model, but with a higher apparent viscosity. In the final stage, condensation of the liquid phase and dissolution of the gas phase lead to total filling of the nanochannel. The observed phenomena are important for understanding the behavior of multiphase systems in nanochannels.     

Processing, Physicochemical, Nutritional, and Organoleptical Properties of Dackere, an African Cereal/Tuber Food Semolina

Dackere is a granulated food made from tubers or cereals flours by women in rural areas of Northern Cameroon. The processing of the semolina faces some constraints that affect the quality and hence the potential to improve the incomes of producers. The objectives of this work were to identify and characterize the processing steps involved in the production of dackere and evaluate the physicochemical and hedonic characteristics of this food. Four groups of farmers consisting of organized and specialized women in dackere processing were used for this purpose and then followed up in their production site. The results obtained revealed that dackere was manufactured from either tubers or cereals based on the available food materials: sweet potato, cassava, corn, millet, fonio, and mixture of sweet potato/corn. Generally, the processes involved included hydrating the flour with water, granulating manually, steam cooking, and eventually drying. All these processes were done in a non-replicable manner using traditional equipment. The physicochemical characterization revealed that dackeres were in general rich in starch (60.63 to 94.99 g/100 g) and poor in proteins (0.77 to 4.42 g/100 g). Digestible starch varied from 34.9 g/100 g for corn dackere to 66.6 g/100 g for cassava and sweet potatoes dackeres, and the index of whiteness varied from 58.8 for sweet potatoes dackere to 75.8 for cassava and corn dackeres. Significant variations were observed among producers. Consumers more appreciated the sweet potato/corn dackere mixture which was associated by principal component analysis to high swallowing ability, taste, and adherence in mouth. However, they were irregular in size and brown in color, characteristics negatively correlated to acceptability.     

A randomized trial comparing fluconazole with amphotericin B for the treatment of candidemia in patients without neutropenia. Candidemia Study Group and the National Institute

BACKGROUND: Amphotericin B has long been the standard treatment for candidemia, but its use is complicated by its toxicity. More recently, fluconazole, a water-soluble triazole with activity against candida species and little toxicity, has become available. We conducted a multicenter randomized trial that compared amphotericin B with fluconazole as treatment for candidemia. METHODS: To be eligible, patients had to have a positive blood culture for candida species, a neutrophil count > or = 500 per cubic millimeter, and no major immunodeficiency. Patients were randomly assigned to receive either amphotericin B (0.5 to 0.6 mg per kilogram of body weight per day) or fluconazole (400 mg per day), each continued for at least 14 days after the last positive blood culture. Outcomes were assessed by a group of investigators blinded to treatment assignment. RESULTS: Of the 237 patients enrolled, 206 met all entry criteria. The most common diagnoses were renal failure, nonhematologic cancer, and gastrointestinal disease. There was no statistically significant difference in outcome: of the 103 patients treated with amphotericin B, 81 (79 percent) were judged to have been treated successfully, as were 72 of the 103 patients treated with fluconazole (70 percent P = 0.22; 95 percent confidence interval for the difference, -5 to 23 percent). The bloodstream infection failed to clear in 12 patients in the amphotericin group and 15 in the fluconazole group; the species most commonly associated with failure was Candida albicans. There were 41 deaths in the amphotericin group and 34 deaths in the fluconazole group (P = 0.20). Intravascular catheters appeared to be the most frequent source of candidemia. There was less toxicity with fluconazole than with amphotericin B. CONCLUSIONS: In patients without neutropenia and without major immunodeficiency, fluconazole and amphotericin B are not significantly different in their effectiveness in treating candidemia.     

Homogeneously catalyzed hydrogenation and gas-liquid chromatographic analysis of the methyl esters of cyclopropene fatty acids

Various concentrations of cyclopropene fatty acids have been determined down to 0.2% by the use of gas liquid chromatographic (GLC) analysis of the methyl esters of fatty acids that have been quantitatively hydrogenated using a homogeneous transition metal complex catalyst. The effectiveness of the use of bromotris(triphenylphosphine)-rhodium(I), Br(P(C₆H₅)₃)₃Rh, as a homogeneous hydrogenation catalyst to convert the cyclopropene ring to a cyclopropane ring has been evaluated and compared with the analogous chloro- and iodo-complexes. The hydrogenation/GLC method of analysis has been compared with the method of titration with hydrogen bromide in benzene and with the method involving the use of high resolution nuclear magnetic resonance (NMR).     

Severity Indices of Personality Problems (SIPP–118) in adolescents: Reliability and validity.

The Severity Indices of Personality Problems (SIPP–118; Verheul et al., 2008) is a self-report questionnaire focusing on core components of (mal)adaptive personality functioning. The SIPP–118 was developed and validated in an adult population. In adult populations, the 16 facets of the SIPP–118 fit into 5 higher order domains: self-control, identity integration, relational capacities, social concordance, and responsibility. In this study we present the 1st psychometric properties of the SIPP–118 in adolescents. We compared the SIPP–118 scores of a patient and a nonpatient sample of adolescents, and compared personality disordered and non-personality disordered adolescents. In addition, the relationship between scores on the SIPP–118 and other clinical instruments (Symptom Checklist—90–Revised; SCL–90–R; Derogatis, 1975; Dimensional Assessment of Personality Pathology—Basic Personality; DAPP–BQ; Livesley & Jackson, 2002) was investigated. The questionnaires were completed by 378 adolescent patients and 389 adolescents in the community. Facets appeared to be homogeneous, as alpha coefficients ranged from .62 to .89, indicating moderate to acceptable reliability. Also, more pathological SIPP–118 scores were found in the patient sample, and more specifically in the personality disordered sample, suggesting that the facet scores of the SIPP–118 can discriminate between various populations (divergent validity). Correlation with other clinical instruments was moderate to high (–.82 to .10). Taken together, the SIPP–118 seems to be a promising instrument measuring personality pathology in adolescents. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Effect of partial discharges on thermal breakdown of oil‐impregnated paper

In this paper, we investigate the effect of partial discharges (PDs) on the thermal breakdown voltage of oil‐impregnated paper (OIP). A disk‐shaped cavity in a stack of paper sheets formed the PD source. The paper sample was exposed to PDs for 18 h. Dielectric spectroscopy measurements were performed on the samples before and after exposure to PD activity. The dielectric spectroscopy results showed a big change in the real and imaginary part of the permittivity. These results were used to calculate the activation energy and heat losses inside the insulation. In order to investigate the possibility of thermal breakdown in the system, the OIP system was simulated by using the FEM software COMSOL Multiphysics. The heat produced by PD pulses and dielectric losses was considered in the simulation. The results show that for the OIP insulation, PD activity can reduce the thermal breakdown voltage by a factor up to 4 times. © 2015 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.     

A rapid density gradient technique for separating polymorphonuclear granulocytes

A gradient separation technique followed by isotonic ammonium chloride haemolysis was compared with two methods for the isolation of polymorphonuclear neutrophils from blood. This technique provided a high yield, excellent purity without lymphocyte and erythrocyte contamination, and made it possible to isolate more than 50 x 10(6) human neutrophils from 15 ml of blood. The polymorphonuclear neutrophils isolated in this way were capable of generating a large amount of reactive oxygen species. This technique for the separation of polymorphonuclear neutrophils is an effective method for in vitro studies.     

Kappa-Opioid Receptor Blockade Ameliorates Obesity Caused by Estrogen Withdrawal via Promotion of Energy Expenditure through mTOR Pathway

Weight gain is a hallmark of decreased estradiol (E2) levels because of menopause or following surgical ovariectomy (OVX) at younger ages. Of note, this weight gain tends to be around the abdomen, which is frequently associated with impaired metabolic homeostasis and greater cardiovascular risk in both rodents and humans. However, the molecular underpinnings and the neuronal basis for these effects remain to be elucidated. The aim of this study is to elucidate whether the kappa-opioid receptor (k-OR) system is involved in mediating body weight changes associated with E2 withdrawal. Here, we document that body weight gain induced by OVX occurs, at least partially, in a k-OR dependent manner, by modulation of energy expenditure independently of food intake as assessed in Oprk1−/−global KO mice. These effects were also observed following central pharmacological blockade of the k-OR system using the k-OR-selective antagonist PF-04455242 in wild type mice, in which we also observed a decrease in OVX-induced weight gain associated with increased UCP1 positive immunostaining in brown adipose tissue (BAT) and browning of white adipose tissue (WAT). Remarkably, the hypothalamic mTOR pathway plays an important role in regulating weight gain and adiposity in OVX mice. These findings will help to define new therapies to manage metabolic disorders associated with low/null E2 levels based on the modulation of central k-OR signaling.