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MA Molina MJ Romero E Abril I Delgado RM Cano F Garrido JM de Pablos ML Garrido 《Canadian Metallurgical Quarterly》1994,39(4):253-256
INTRODUCTION: Heterozygous beta-thalassemia manifests hematologically with microcytosis, reduced red blood cell hemoglobin concentration and high hemoglobin A2 levels. Almost all molecular alterations are due to point mutations. We attempt to determinate the frequency of that mutations in the Oriental Andalusia Area, and its relationship with the hematological phenotype. PATIENTS AND METHODS: We have studied 45 heterozygous patients. DNA samples were amplified by PCR, using the printers CD7 and HI1. A 16 Kb fragment corresponding to beta globin gene was obtained and analyzed by Dot Blot assay and hybridized with allelic specific oligonucleotide (ASO) probes to detect the 6 more frequent mutations found in the South of Spain. RESULTS: Codon 39 nonsense mutation (31.1%) was the most frequent finding followed by IVS-1 NT 110 (26.7%). The relationship between hematological parameters and molecular mutations concluded that IVS-I NT 6 mutation developed a minimal anemia. DISCUSSION: From the practical point of view, this study indicates that we were able to detect more than 90% of heterozygous beta-tal. with 5 out of 6 ASO probes used in this work. Thus, our data also provides a further implication in prenatal diagnosis. 相似文献
3.
María P. Albano Liliana B. Garrido 《Journal of Materials Synthesis and Processing》2002,10(4):211-218
Si3N4powders coated with 6 wt% Y2O3and 4 wt% Al2O3were prepared by coprecipitation. The resulting powders were dispersed in water at different pH values and with addition of various amounts of ammonium polyacrylate (NH4PA) to produce 32 vol% slips. The influence of the amount of NH4PA solution added and pH on the rheological properties of 32 vol% coated Si3N4slips were studied. In addition, the sintered density of cast samples was determined and related to the degree of slip dispersion. The adsorption of the NH4PA on the coated particle surface was rather high and the surface became saturated near 0.86 mg/m2at pH 9.2. High NH4PA concentrations (1.7–3 wt%) were necessary to obtain well dispersed 32 vol% coated Si3N4slips at pH 9.2. The best stabilization was obtained with the addition of 2.3 wt% NH4PA; in this condition, the viscosity reached a minimum value of 35 mPa.s at 100 s–1. The slip viscosity increased with increasing pH from 9.2 to 10.2. Slips with low viscosities gave a more dense packing of cast samples and consequently higher sintered density values. 相似文献
4.
J Santafe J Segarra M Garrido V Pablo MJ Martinez de Ibarreta 《Canadian Metallurgical Quarterly》1993,24(2):471-478
1. Interaction between topical isoprenaline and timolol on intraocular pressure was studied in conscious rabbits through changes of the dose-response curves. 2. Both drugs caused a dose-dependent fall of intraocular pressure mainly by reducing the aqueous humour formation. 3. When combined, timolol increased the response to submaximal concentrations of isoprenaline as well as EC50 (from 2.06 x 10(-3) M to 3.63 x 10(-3) M) and slope (from 1.21 to 1.89), but peak response to isoprenaline remained unchanged. 4. Discordance between experimental and theoretical additive curves as well as an interaction index smaller than 1 show that the interaction between both drugs is synergistic. 相似文献
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In this paper, we propose a stable neurovisual servoing algorithm for set-point control of planar robot manipulators in a fixed-camera configuration an show that all the closed-loop signals are uniformly ultimately bounded (UUB) and converge exponentially to a small compact set. We assume that the gravity term and Jacobian matrix are unknown. Radial basis function neural networks (RBFNNs) with online real-time learning are proposed for compensating both gravitational forces and errors in the robot Jacobian matrix. The learning rule for updating the neural network weights, similar to a back propagation algorithm, is obtained from a Lyapunov stability analysis. Experimental results on a two degrees of freedom manipulator are presented to evaluate the proposed controller. 相似文献
7.
Aqueous tape casting of yttria stabilized zirconia 总被引:2,自引:0,他引:2
Tape casting process was used to produce yttria stabilized zirconia (YSZ) substrates in an aqueous system with poly(vinylalcohol) (PVA) and glycerine as binder and plasticizer, respectively. Various compositions of YSZ slips with different amounts of PVA and glycerine and consequently different solid/liquid ratios were prepared. The influence of the slip composition on the rheological properties of the slips was studied. In addition, the effect of the slip composition on the properties of the green and sintered tapes was investigated. PVA and glycerine did not affect the dispersion properties of the YSZ powder. Glycerine additions enhanced the flexibility of the green tapes but also produced a decrease in the tensile strength. The increase in the PVA content increased the tensile strength but resulted in a markedly decrease in the green density of the tapes. A correlation between the green and sintered density was found. The anisotropic sintering shrinkage parallel and perpendicular to the casting direction increased with increasing the PVA content. The slip compositions with 5 wt% PVA produced green tapes with satisfactory tensile strength. They had the highest sintered density, the lower sintering shrinkage and the lesser shrinkage anisotropy. 相似文献
8.
L. Thom A. Gentils J. Jagielski F. Garrido T. Thom 《Nuclear instruments & methods in physics research. Section B, Beam interactions with materials and atoms》2006,250(1-2):106-113
The evaluation of the damage generated in ceramic oxides exposed to radiations is a major challenge in many technological domains which cover electronic, space and nuclear industries. Thus there is a great need of data concerning the production and recovery of radiation damage in this type of materials. This work aims to present a short review of the main structural and chemical modifications recently observed in yttria-stabilized zirconia and magnesium aluminate spinel that are submitted to ion bombardment and thermal treatments. 相似文献
9.
PF Eire D Vallejo JL Sastre MA Rodriguez M Garrido 《Canadian Metallurgical Quarterly》1998,33(12):1820-1821
Mesenteric venous thrombosis (MVT) after appendicectomy has not been reported in the pediatric literature. This complication is associated with significant morbidity and mortality rates in adults. The report of this case attempts to analyze the features found in an appendicectomized child documented by radiological examination and surgery before and after starting heparin therapy. Anticoagulation treatment should be started as soon as possible and continued for a long time to decrease the risk of relapse. 相似文献
10.
Lorena Cuenca-Bermejo Elisa Pizzichini Valeria C. Gonalves María Guilln-Díaz Elena Aguilar-Moino Consuelo Snchez-Rodrigo Ana-María Gonzlez-Cuello Emiliano Fernndez-Villalba María Trinidad Herrero 《International journal of molecular sciences》2021,22(9)
The diurnal rodent Octodon degus (O. degus) is considered an attractive natural model for Alzheimer’s disease and other human age-related features. However, it has not been explored so far if the O. degus could be used as a model to study Parkinson’s disease. To test this idea, 10 adult male O. degus were divided into control group and MPTP-intoxicated animals. Motor condition and cognition were examined. Dopaminergic degeneration was studied in the ventral mesencephalon and in the striatum. Neuroinflammation was also evaluated in the ventral mesencephalon, in the striatum and in the dorsal hippocampus. MPTP animals showed significant alterations in motor activity and in visuospatial memory. Postmortem analysis revealed a significant decrease in the number of dopaminergic neurons in the ventral mesencephalon of MPTP animals, although no differences were found in their striatal terminals. We observed a significant increase in neuroinflammatory responses in the mesencephalon, in the striatum and in the hippocampus of MPTP-intoxicated animals. Additionally, changes in the subcellular expression of the calcium-binding protein S100β were found in the astrocytes in the nigrostriatal pathway. These findings prove for the first time that O. degus are sensitive to MPTP intoxication and, therefore, is a suitable model for experimental Parkinsonism in the context of aging. 相似文献