Divide-and-Conquer Matrisome Protein (DC-MaP) Strategy: An MS-Friendly Approach to Proteomic Matrisome Characterization

Currently, the extracellular matrix (ECM) is considered a pivotal complex meshwork of macromolecules playing a plethora of biomolecular functions in health and disease beyond its commonly known mechanical role. Only by unraveling its composition can we leverage related tissue engineering and pharmacological efforts. Nevertheless, its unbiased proteomic identification still encounters some limitations mainly due to partial ECM enrichment by precipitation, sequential fractionation using unfriendly-mass spectrometry (MS) detergents, and resuspension with harsh reagents that need to be entirely removed prior to analysis. These methods can be technically challenging and labor-intensive, which affects the reproducibility of ECM identification and induces protein loss. Here, we present a simple new method applicable to tissue fragments of 10 mg and more. The technique has been validated on human ovarian tissue and involves a standardized procedure for sample processing with an MS-compatible detergent and combined centrifugation. This two-step protocol eliminates the need for laborious sample clarification and divides our samples into 2 fractions, soluble and insoluble, successively enriched with matrisome-associated (ECM-interacting) and core matrisome (structural ECM) proteins.     

Bibliometric analysis of the evolution of biochar research trends and scientific production

Clean Technologies and Environmental Policy - Biochar has caught great attention over the last decade, yielding a large number of publications in a broad range of disciplines. This scientometric...     

RF MEMS continuous reversible variable inductor based on a microfluidic network

In this work, RF MEMS continuous reversible variable inductor has been fabricated by using microelectronic technology and lamination process. We review, evaluate and compare this variable inductor with other work. The proposed inductor is a dual circular coil and has an inductance of few nH. The fundamental idea is to place a liquid droplet between the metal turns of a coil in order to modify the capacitive/resistive coupling between metal tracks and hence to change the stored magnetic energy. The SU-8 resin was used to realize the microfluidic channels and Au as metallic tracks. To prove the reversibility of the inductor, two cases were studied: filling and emptying of channels. The tuning range of the inductance is approximately 107 % at 1.6 GHz, making these devices very suitable as building blocks in many RF applications.     

Analysis of e-business models in real estate

We use Canvas Application Framework to analyze e-business models of Realestate.yahoo.com, Yahoo.com, Redfin.com, Realtor.com, Trulia.com, Zillow.com, and Craigslist.com. We investigate the similarities and differences among e-business models and how e-business model differentiation affects company success. We looked at which business model components, such as distribution channels, business partnerships, attracting customers, revenue streams, etc., are important. We further investigate whether the web-advertising, brokerage, virtual value-chain, or the diversified internet business model is used by each company, and which ones are more successful. We discover similarities and differences among e-business models. Differences include the use of revenue streams for generating revenue, the extent of partnerships, and the role they play in a company’s success.     

Virtual-water content of agricultural production and food trade balance of Tunisia

This article is devoted to the assessment of Tunisian agricultural production and food trade balance water-equivalent. A linear regression model relating annual rainfall to crop yields is developed to estimate the agricultural production water-equivalent. Its implementation is based on national data for crop and animal production, leading to food demand water-equivalent quantification. Results highlight the relationship between agricultural and water policies and provide a picture of food security in the country in relation to local agricultural production, and to virtual water fluxes related to foodstuffs trade balance.     

Fusion of thermal infrared and visible spectra for robust moving object detection

The detection of moving objects is a crucial step for many video surveillance applications whether using a visible camera (VIS) or an infrared (IR) one. In order to profit from both types, several fusion methods were proposed in the literature: low-level fusion, medium-level fusion and high-level fusion. The first one is the most used for moving objects’ detection in IR and VIS spectra. In this paper, we present an overview of the different moving object detection methods in IR and VIS spectra and a state of the art of the low-level fusion techniques. Moreover, we propose a new method for moving object detection using low-level fusion of IR and VIS spectra. In order to evaluate quantitatively and qualitatively our proposed method, three series of experiments were carried out using two well-known datasets namely “OSU Color-Thermal Database” and “INO-Database”; the results of these evaluations show promising results and demonstrate the effectiveness of the proposed method.     

Runoff Estimation for an Ungauged Catchment Using Geomorphological Instantaneous Unit Hydrograph (GIUH) and Copulas

A methodology is proposed to apply Geomorphological Instantaneous Unit Hydrograph to ungauged basins using Monte Carlo Simulations and copulas. The effective rainfall, input of GIUH is assumed to be unknown; it is estimated with infiltration index method (?-index). Correlations are detected between this index and the characteristics of rainfall. They are modeled with copulas, and are used to derive effective rainfall hyetographs. The generated hydrographs from GIUH are analyzed and give statistically the same results: dispersion and variability for all studied characteristics (volume, peak discharge, peak time and base time). However, only these hydrographs derived from ? conditioned to maximum intensity distribution allow reconstituting the observed hydrographs. Moreover comparing the series of order statistics of interest output and observed series, leads to decide on the representative hydrograph of the catchment behavior.     

Compositional characteristics and aromatic profile of date palm seeds from seven varieties grown in Tunisia

This paper reports a discrimination study based on the physico‐chemical characteristics, fatty acids and profile of volatile compounds of the seeds from seven date palm varieties (Phoenix dactylifera L.) grown in Tunisia. Date seeds contained 10.49–14.76% moisture; 6.28–11.2% fat (on a dry weight basis); 2.67–12.85% protein; 0.91–6.06% reducing sugar; 0.61–2.98% sucrose and 0.97–1.17% ash. Gas liquid chromatography revealed that the oil fraction of the date palm seeds contained eighteen fatty acids, with oleic acid (30.77–42.50%) and lauric acid (18.51–27.48%) as the main unsaturated and saturated ones. Volatile profile showed differences among varieties. In total, forty‐five compounds were identified, mainly alcohols, aldehydes and unsaturated hydrocarbons ones. This study provides evidence that the seeds of date may be a potential source of valuable nutrients with interesting functionality.     

Osmotic Dehydration Kinetics of Pomegranate Seeds Using Date Juice as an Immersion Solution Base

Pomegranate seeds were osmodehydrated using date juice added with sucrose (final °Brix, 55) as immersion solution. The kinetics of osmotic dehydration showed that the most significant changes of mass transfer took place during the first 20 min of the process, regardless of date juice varieties. During this time, seed water loss and solid gain were estimated to be ∼39% and ∼6%, respectively. After 20 min of the process, the percentage of water loss and solid gain varied slightly and ranged on average close to ∼40% and ∼9%, respectively. During osmotic dehydration, there was a leaching of natural solutes from seeds into the solution, which is quantitatively not negligible, and might have an important impact on the sensorial and nutritional value of seeds and date juices. Both scanning electron microscopy and texture (compression) analysis revealed that osmotic dehydration process induced modifications of seed texture and cell structure. Sucrose was found to be the essential element which influences the texture of seed and the viscosity of date juice. Additionally, natural sugar present in date juice permits substituting 35% of the total quantity of sucrose added to the osmotic solution.     

Synergistic effect of organoclay fillers based on fluorinated surfmers for preparation of polystyrene nanocomposites

Novel fluorinated reactive surfactants were used for the organic modification of monmorillonite clays. These organoclays were used for the preparation of polystyrene?clay nanocomposites by in‐situ free radical polymerization. Reference systems based on hydrocarbon homologous surfmer and nonpolymerizable surfactants were also used to deduce the effect of the fluorine moiety and the polymerizable function on the morphology and thermal stability of the prepared nanocomposites. Different structural parameters of the surfactants were investigated and modulated for the clay modification including: the nature of surfactant (surfmer/classical surfactant, fluorinated, or hydrocarbonated), the length of the fluorinated chain as well as the length of the hydrocarbon spacers linking the ammonium head to the fluorine chain or the polymerizable acrylic function. Wide angle‐X‐ray scattering (WAXD), thermogravimetric analysis (TGA), and electronic microcopies (TEM and SEM) were used to establish a structure‐morphology, thermal properties relationships, and to highlight the key parameters governing the exfoliation process. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 42347.