Loosening mechanism of threaded fastener for complex structures

Journal of Mechanical Science and Technology - Threaded fasteners are widely used in mechanical structures primarily owing to their easy disassembly for maintenance and low cost. However, the...     

Expression,purification, and characterization of human intestinal maltase secreted from Pichia pastoris

A gene encoding human intestinal maltase (HMA) was successfully expressed in Pichia pastoris under the control of the methanol-induced alcohol oxidase (AOX1) promoter. The secreted recombinant HMA fused with a His₆-tag was produced (150 U/L) and was easily purified from culture supernatants in a 3-step diafiltration, ultrafiltration, and affinity column chromatography protocol. The specific activity of the purified HMA was 16.8 U/mg. Endoglycosidase H digestion of the protein showed that the recombinant HMA was N-glycosylated. The purified HMA was maximally active at pH 6.5 and stable (≥90%) up to 65°C. The kinetic parameters K _m and V _max were 3.3±0.25 mM maltose and 61.9±2 U/mg, respectively.     

Improvement of flame stability and NOx reduction in hydrogen-added ultra lean premixed combustion

Lean premixed combustion is a well known method in gas turbine combustors that can reduce fuel consumption and decrease flame temperature. In lean premixed flames, flame instabilities can occur because the combustion takes place near the lean flammable limit. For the purpose of increasing flame stability, a small amount of hydrogen was added into a fuel, which has ultra low lean flammable limit. The extinction stretch rate increased and total equivalence ratio at extinction decreased with hydrogen addition; consequently, ultra lean premixed combustion was possible and flame stability could be achieved at low temperature conditions. The NO_x emission increased with hydrogen addition for the same stretch rate and equivalence ratio, but the extinction stretch rate and lean flammability limit was enlarged. Consequently, NO_x emission decreased with hydrogen addition in the near extinction conditions. Hydrogen addition could improve flame stability and reduce NO_x emission in ultra lean premixed combustion. This paper was recommended for publication in revised form by Associate Editor Ohchae Kwon Dr. Eun-Seong Cho received his B.S. and M.S. degrees in Mechanical Engineering from Hanyang University, Korea, in 1996 and 1998, respectively. He then received his Ph.D. degree from Seoul National University, Korea, in 2005. He was a principal engineer of KD Navien research center and currently a research associate at Delft University of Technology, The Netherlands. His research interests include eco-friendly clean combustion technology, new and renewable energy systems. Prof. Suk Ho Chung received his B.S. degree from Seoul National University, Korea, in 1976 and Ph.D. degree in Mechanical Engineering from Northwestern University, USA, in 1983. He is a Professor since 1984 in the School of Mechanical and Aerospace Engineering at Seoul National University in Seoul, Korea. His research interests cover combustion fundamentals, pollutant formation, laser diagnostics, and plasma-assisted combustion.     

Numerical evaluation of NOx mechanisms in methane-air counterflow premixed flames

The control of nitrogen oxides (NO_x) has been a major issue in designing combustion systems, since NO_x play a key role in ozone depletion and the generation of photochemical smog. The characteristics of NO_x emission can be essential information for the development of a clean combustor having suitable reduction methodologies. In the present study, NO_x emission characteristics were evaluated numerically, accounting for the effect of equivalence ratio, stretch rate, pressure, and initial temperature. In general, peak NO_x emission appeared near the equivalence ratio of unity case, and NO_x emission increased with pressure and initial temperature due to the temperature sensitivity in NO_x mechanism. NO_x decreased with stretch rate due to the decrease in residence time in high temperature region. Furthermore, the thermal and prompt mechanisms were evaluated with equivalence ratio for two calculation methods. The conventional methods ignore the interaction of coupled mechanism of thermal and prompt NO_x. The reaction path diagram was introduced to understand effective reaction pathways in various conditions. This paper was recommended for publication in revised form by Associate Editor Kyoung Doug Min Dr. Eun-Seong Cho received his B.S. and M.S. degrees in Mechanical Engineering from Hanyang University, Korea, in 1996 and 1998, respectively. He then received his Ph.D. degree from Seoul National University, Korea, in 2005. He was a principal engineer of KD Navien research center and currently a research associate at Delft University of Technology, The Netherlands. His research interests include eco-friendly clean combustion technology, new and renewable energy systems. Prof. Suk Ho Chung received his B.S. degree from Seoul National University, Korea, in 1976 and Ph.D. degree in Mechanical Engineering from Northwestern University, USA, in 1983. He is a Professor since 1984 in the School of Mechanical and Aerospace Engineering at Seoul National University in Seoul, Korea. His research interests cover combustion fundamentals, pollutant formation, laser diagnostics, and plasma-assisted combustion.     

Characteristics of NOx emission with flue gas dilution in air and fuel sides

Flue gas recirculation (FGR) is a method widely adopted to control NO_x in combustion system. The recirculated flue gas decreases flame temperature and reaction rate, resulting in the decrease in thermal NO production. Recently, it has been demonstrated that the recirculated flue gas in fuel stream, that is, the fuel induced recirculation (FIR), could enhance a much improved reduction in NO_x per unit mass of recirculated gas, as compared to the conventional FGR in air. In the present study, the effect of FGR/FIR methods on NO_x reduction in turbulent swirl flames by using N₂ and CO₂ as diluent gases to simulate flue gases. Results show that CO₂ dilution is more effective in NO reduction because of large temperature drop due to the larger specific heat of CO₂ compared to N₂ and FIR is more effective to reduce NO emission than FGR when the same recirculation ratio of dilution gas is used.     

Biochemical characterization of dextranase from Arthrobacter oxydans and its cloning and expression in Escherichia coli

Appreciably elevated levels of dextranase from Arthrobacter oxydans (AODex) isolated from sugar-cane farm soil was resulted from the culture on the Luria-Bertani (LB) medium containing 1%(w/v) soluble starch, glycerol, or dextran. The responsible gene (aodex) was cloned, its nucleotide sequence was determined, and expression of the encoded protein was achieved in Escherichia coli. An open reading frame was composed of 1,863 bp putatively encoding a 68.3 kDa protein. Recombinant A. oxydans dextranase (rAODex) was purified about 16 fold by nickel-nitrilotriacetic acid affinity column chromatography; K _m value for dextran T2000 was 0.85 mg/mL (w/v). AODex treatment of stale sugar cane juice resulted in a yield of square and light-colored sugar crystals.     

Quantification of extinction mechanism in counterflow premixed flames

The extinction mechanisms of stretched premixed flames have been investigated numerically for the fuels of CH₄, C₃H₈, H₂, CO and for the mixture fuels of CH₄+H₂ and CO+H₂ by adopting symmetric double premixed flames in a counterflow configuration. The local equilibrium temperature concept was used as a measure of energy loss or gain in order to quantify the extinction mechanism by preferential diffusion and/or incomplete reaction. The energy loss ratio from preferential diffusion arising from non-unity Lewis number and the loss ratio from incomplete reaction were calculated at various equivalence ratios near flame extinction. The results showed that the extinction of lean H₂, CH₄, CH₄+H₂, CO+H₂, and rich C₃H₈ premixed flames was caused by incomplete reaction due to insufficient reaction time, indicating that the effective Lewis number was smaller than unity, while the effect of preferential diffusion resulted in energy gain. However, the extinction of rich H₂, CH₄, CH₄+H₂, CO+H₂, and lean C₃H₈ premixed flames was affected by the combined effects of preferential diffusion and incomplete reaction indicating that the effective Lewis number was larger than unity. In CO premixed flames, incomplete reaction was dominant in both lean and rich cases due to the effective Lewis number close to unity. The effect of H2 mixing to CO is found to be quite significant as compared to CH₄+H₂ cases, which can alter the flame behavior of CO flames to that of H₂.     

An experimental study on combustion processes and nox emission characteristics of the air-staged burner

The combustion processes and emission characteristics in air-staged burner have been experimentally studied. The light fuel oil doped with pyridine(C₅H₅N) is used to investigate the fuel NO_X emission characteristics. Experiments are carried out for a wide range of operating conditions of single-staged and multi-staged burner. The detailed discussions are made for the flame structure of the air-staged burner as well as effects of excess air ratios, staged air flow percentage, and spray conditions on flame pattern and NO_X emission characteristics.     

Numerical study on no emission with flue gas dilution in air and fuel sides

Flue gas recirculation (FGR) is widely adopted to control NO emission in combustion systems. Recirculated flue gas decreases flame temperature and reaction rate, resulting in the decrease in thermal NO production. Recently, it has been demonstrated that the recirculated flue gas in fuel stream, that is, the fuel induced recirculation (FIR), could enhance much improved reduction in NO per unit mass of recirculated gas, as compared to conventional FGR in air. In the present study, the effect of dilution methods in air and fuel sides on NO reduction has been investigated numerically by using N3 and CO2 as diluent gases to simulate flue gases. Counterflow diffusion flames were studied in conjunction with the laminar flamelet model of turbulent flames. Results showed that CO2 dilution was more effective in NO reduction because of large temperature drop due to the larger specific heat of CO2 compared to N₂. Fuel dilution was more effective in reducing NO emission than air dilution when the same recirculation ratio of dilution gas was used by the increase in the nozzle exit velocity, thereby the stretch rate, with dilution gas added to fuel side.