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排序方式: 共有768条查询结果,搜索用时 15 毫秒
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Chinh Ngo Radhika Mehta Kanchan Aggarwal Audrey G. Fikes Dr. Ines C. Santos Dr. Sylvester M. Greer Prof. Dr. Emily L. Que 《Chembiochem : a European journal of chemical biology》2019,20(8):1003-1007
One-third of all proteins are estimated to require metals for structural stability and/or catalytic activity. Desthiobiotin probes containing metal binding groups can be used to capture metalloproteins with exposed active-site metals under mild conditions so as to prevent changes in metallation state. The proof-of-concept was demonstrated with carbonic anhydrase (CA), an open active site, Zn2+-containing protein. CA was targeted by using sulfonamide derivatives. Linkers of various lengths and structures were screened to determine the optimal structure for capture of the native protein. The optimized probes could selectively pull down CA from red blood cell lysate and other protein mixtures. Pull-down of differently metallated CAs was also investigated. 相似文献
3.
Xueming Huang Gordon I. McCalla Jim E. Greer Eric Neufeld 《User Modeling and User-Adapted Interaction》1991,1(1):87-115
A user/student model must be revised when new information about the user/student is obtained. But a sophisticated user/student model is a complex structure that contains different types of knowledge. Different techniques may be needed for revising different types of knowledge. This paper presents a student model maintenance system (SMMS) which deals with revision of two important types of knowledge in student models: deductive knowledge and stereotypical knowledge. In the SMMS, deductive knowledge is represented by justified beliefs. Its revision is accomplished by a combination of techniques involving reason maintenance and formal diagnosis. Stereotypical knowledge is represented in the Default Package Network (DPN). The DPN is a knowledge partitioning hierarchy in which each node contains concepts in a sub-domain. Revision of stereotypical knowledge is realized by propagating new information through the DPN to change default packages (stereotypes) of the nodes in the DPN. A revision of deductive knowledge may trigger a revision of stereotypical knowledge, which results in a desirable student model in which the two types of knowledge exist harmoniously. 相似文献
4.
Technology can take many forms from the development of sophisticated electronics to the application of basic procedural principles. Technology transfer can also take several forms, however, in this case, it is the transfer of technological principles between application domains that is of primary interest. This paper presents a framework (the Five ‘M’s framework) that can be used to consider the likely success of technology transfer. The framework is based upon a sociotechnical system approach. The framework is illustrated with an evaluation of the introduction of intelligent cruise control into motor vehicles. It is argued that this is a technological concept first developed as an aerospace application in the form of the autopilot. In this case, it is argued that the transfer of this technology in its current form may potentially compromise safety. 相似文献
5.
CM Wheeler CA Parmenter WC Hunt TM Becker CE Greer A Hildesheim MM Manos 《Canadian Metallurgical Quarterly》1993,20(5):286-289
OBJECTIVES: To confirm the risk factors for genital human papillomavirus (HPV) infection. GOAL OF THIS STUDY: To investigate risk factors for HPV detection apart from the correlated risk factors for cervical neoplasia. STUDY DESIGN: Cervical human papillomavirus (HPV) DNA was assessed in 357 cytologically normal women attending the University of New Mexico student health center. Cervical swab samples were obtained for HPV DNA detection and typing using a PCR-based DNA amplification system. Possible determinants of cervical HPV were examined including age, ethnicity, history of sexually transmitted disease, oral contraceptive use, smoking, age at first intercourse, lifetime number of sex partners, marital status, and history of pregnancy. RESULTS: A 44.3% overall prevalence of cervical HPV was observed. On univariate analysis, factors associated with increasing HPV prevalence included higher lifetime number of sex partners and single marital status. After adjustment for potential confounding variables, we found that HPV prevalence increased with higher lifetime number of sexual partners. CONCLUSION: These findings, along with those from the companion reports in this issue of the journal, support the sexual route of transmission of the virus. 相似文献
6.
Randall Billi; Moss Helen E.; Rodd Jennifer M.; Greer Mike; Tyler Lorraine K. 《Canadian Metallurgical Quarterly》2004,30(2):393
Patients with category-specific deficits have motivated a range of hypotheses about the structure of the conceptual system. One class of models claims that apparent category dissociations emerge from the internal structure of concepts rather than fractionation of the system into separate substores. This account claims that distinctive properties of concepts in the living domain are vulnerable because of their weak correlation with other features. Given the assumption that mutual activation among correlated properties produces faster activation in the normal system, the authors predicted a disadvantage for the distinctive features of living things for unimpaired adults. Results of a speeded feature verification study supported this prediction, as did a computational simulation in which networks mapped from orthography to semantics. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
7.
The crystallization of Pd40Ni40P20 glass, produced by melt-spinning in air, has been studied by optical metallography. Crystallization is predominantly from the surface and is more prevalent on the wheel-side. The non-uniformity is attributed to variation in quench rate during production. A three-stage anneal permits crystals which have nucleated at the surface to be identified and their size distribution to be analysed. The surface nucleation is heterogeneous and appears to be hindered by mild oxidation. The annealing atmosphere markedly affects the surface crystallization behaviour, as does removal of the original ribbon surface. When nucleation is sparse, partial crystallization causes the development of noticeable relief on the sample surfaces. 相似文献
8.
George Grant Fiona Greer Norma H. McKenzie Arpad Pusztai 《Journal of the science of food and agriculture》1985,36(5):409-414
In a series of 10-day pair feeding experiments it was found that the nutritional value of diets containing beans was essentially the same for rats aged between 30 and 123 days. Thus net protein utilisation (NPU) values of 25–39 on diets containing Processor bean (35 g protein kg?1) + egg albumin (65g protein kg?1) were obtained. As food intakes were considerably reduced when rats were fed diets containing more than 35g protein kg?1 of Processor bean, the measurement of protein utilisation became increasingly more difficult. The severe disruption of the brush borders of duodenal and jejunal enterocytes, originally observed when bean-containing diets were fed to young (30-day-old) rats was also found with rats up to the age of 120 days on similar diets. Similarly, the development of circulating anti-lectin antibodies in the animals showed no age dependence within the age limits investigated. It was also shown that oral immunisation did not protect the rats from the effects of toxicity and that the immune response was a result of continuous absorption of lectin throughout the feeding period. Thus the extent and the mechanism of toxicity of Phaseolus vulgaris bean lectins were found not to be dependent on the age or maturity of the animal. 相似文献
9.
We report here the identification of a new precipitate phase in thin-film Al-4wt.%Cu metallization used for interconnects
on integrated circuits. The phase is based on a trigonal distortion of a face centered cubic lattice. Computer simulation
of electron diffraction intensities suggests that the basis structure is isomorphous with Al2Ca but with a large and ordered population of vacancies on Cu sites. The reason for the formation of the new phase and its
implications for electromigration reliability are discussed. 相似文献
10.
Alpass Fiona; Flett Ross; Humphries Steve; Massey Claire; Morriss Stuart; Long Nigel 《Canadian Metallurgical Quarterly》2004,11(3):270
Previous studies have identified a number of different farm stressors and have reported gender and age differences in levels of stress. This study aimed to determine stress experienced by New Zealand dairy farmers, particularly in relation to adoption of new technology and its relationship to age and gender. Measures of farm stress and adoption of technology were completed by 985 dairy farmers. The highest levels of stress were reported for time pressures, machinery breakdown, weather, and government policies. Stress related to new technologies occurred rarely. Women reported more stress and higher levels of stress for understanding new technologies. There was also an increase in severity of stress with age related to new technologies. Overall, stress levels were moderate and may reflect favorable economic conditions. The findings suggest a need to tailor stress interventions to meet specific needs. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献