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排序方式: 共有318条查询结果,搜索用时 250 毫秒
1.
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Thermal conductivity of apple and potato samples was measured by means of a specifically designed apparatus based on Fitch's method. The thermal conductivity was determined at various moisture contents at the sample mean temperature of 30 °C. As expected the conductivity decreases with the decrease in moisture content. Thermal conductivity data were correlated with moisture content using a straight line. 相似文献
3.
The inhibition of squalene-hopene cyclase (SHC) (E.C. 5.4.99.-), an enzyme of bacterial membranes catalyzing the formation
of pentacyclic sterol-like triterpenes, was studied by using different classes of compounds originally developed as inhibitors
of oxidosqualene cyclase (OCS) (E.C. 5.4.99.7), the enzyme of eukaryotes responsible for the formation of tetracyclic precursors
of sterols. The mechanism of cyclization of squalene by SHC, beginning with a protonation of the 2,3 double bond by an acidic
residue of the enzyme, followed by a series of electrophilic additions of the carbocationic intermediates to the double bonds,
is similar to the mechanism of cyclization of 2,3-oxidosqualene by OSC. The inhibitors studied included: (i) analogs of the
carbocationic intermediates formed during cyclization, such as aza-analogs of squalene and 2,3-oxidosqualene; (ii) affinity-labeling
inhibitors bearing a methylidene reactive group; and (iii) vinyldioxidosqualenes and vinylsulfide derivatives of the substrates.
Comparison of the results obtained with the two enzymes, SHC and OSC, showed that many of the most effective inhibitors of
OSC were also able to inhibit SHC, while some derivatives acted as specific inhibitors. Differences could be easily explained
on the basis of the different substrate specificity of the two enzymes. 相似文献
4.
Oliaro-Bosso S Schulz-Gasch T Balliano G Viola F 《Chembiochem : a European journal of chemical biology》2005,6(12):2221-2228
A structural model of Saccharomyces cerevisiae oxidosqualene cyclase (SceOSC) suggests that some residues of the conserved sequence Pro-Ala-Glu-Val-Phe-Gly (residues 524-529) belong to a channel constriction that gives access to the active-site cavity. Starting from the SceOSC C457D mutant, which lacks the cysteine residue next to the catalytic Asp456 residue Cys457 has been replaced but Asp456 is still there, we prepared two further mutants where the wild-type residues Ala525 and Glu526 were individually replaced by cysteine. These mutants, especially E526C, were very sensitive to the thiol-reacting agent dodecyl-maleimide. Moreover, both the specific activity and the thermal stability of E526C were severely reduced. A similar decrease of the enzyme functionality was obtained by replacing Glu526 with alanine, while substitution with the conservative residues aspartate or glutamine did not alter catalytic activity. Molecular modeling of the yeast wild-type OSC and mutants on the template structure of human OSC confirms that the channel constriction is an important aspect of the protein structure and suggests a critical structural role for Glu526. 相似文献
5.
Franco Cicirelli Angelo Furfaro Andrea Giordano Libero Nigro 《Simulation Modelling Practice and Theory》2011,19(1):283-300
RePast is a popular agent toolkit with proven capabilities to fulfil the modelling challenges of large multi-agent systems (MASs). The toolkit, though, is normally used on a standalone workstation and therefore its practical use can be constrained (in space and time) by the limited available computing resources. This paper proposes an original approach – HLA_ACTOR_REPAST – aimed to distributing RePast models for high-performance simulation of complex scalable models. Novel in HLA_ACTOR_REPAST is an exploitation of a lean actor infrastructure implemented in Java. Actors bring to RePast agents such features as migration, location-transparent naming, efficient communications, and a control-centric framework. Actors can be orchestrated by an in-the-large custom control structure which is in charge of ensuring the necessary message causality constraints. Distribution and time management concerns depend on the IEEE standard HLA middleware. The paper first discusses details of the software engineering process underlying the development of HLA_ACTOR_REPAST. The mapping techniques, assisted by Java text annotations and aspect-oriented programming, try to minimize “code intrusions” in the original model and favour model transparency. The paper then furnishes some experimental data which witness the good performance results achieved by applying HLA_ACTOR_REPAST to a distributed version of a classic MAS benchmark model. 相似文献
6.
7.
During the last few years, the proliferation of miniaturised devices with networking capabilities has provided the technological grounds for pervasive networking environments. It is not visionary to foresee a world of pervasive devices embedded in the environment interacting between them, and with those carried by users, via wireless communications. In addition, fostered by the diffusion of small-size, computational-rich mobile devices, the way content is generated, and accessed is changing with respect to the legacy-Internet paradigm. An ever-increasing share of the Internet content is generated directly by the users, and shared on the network (following the User-Generated Content model). While today the legacy Internet is still used to share user-generated content, it is reasonable to envision that pervasive networking technologies will represent the natural platform to support this new model. This will result in content being distributed on users’ devices rather than on centralised servers on the Internet, and in users creating ad hoc networks to share content. The p2p paradigm is particularly suitable for this scenario, because communications will occur directly among users, instead of being necessarily mediated by centralised servers. Motivated by these remarks, in this work we focus on p2p multicast services over ad hoc networks aimed at sharing content among groups of users interested in the same topics. Specifically, starting from a reference solution in legacy wired networks (Scribe), we design a cross-layer optimised protocol (XScribe) that addresses most of the Scribe problems on ad hoc networks. XScribe exploits cross-layer interactions with a proactive routing protocol to manage group membership. Furthermore, it uses a lightweight, structureless approach to deliver data to group members. By jointly using experimental results and analytical models, we show that, with respect to Scribe, XScribe significantly reduces the packet loss and the delay experienced by multicast receivers, and increases the maximum throughput that can be delivered to multicast groups. 相似文献
8.
Silvia Franco Elena Buratti Valentina Nigro Emanuela Zaccarelli Barbara Ruzicka Roberta Angelini 《International journal of molecular sciences》2021,22(8)
The phase behaviour of soft colloids has attracted great attention due to the large variety of new phenomenologies emerging from their ability to pack at very high volume fractions. Here we report rheological measurements on interpenetrated polymer network microgels composed of poly(N-isopropylacrylamide) (PNIPAM) and polyacrylic acid (PAAc) at fixed PAAc content as a function of weight concentration. We found three different rheological regimes characteristic of three different states: a Newtonian shear-thinning fluid, an attractive glass characterized by a yield stress, and a jamming state. We discuss the possible molecular mechanisms driving the formation of these states. 相似文献
9.
Shah Ghazanfar Ali Polette Arnaud Pernot Jean-Philippe Giannini Franca Monti Marina 《Engineering with Computers》2021,37(4):2891-2909
Engineering with Computers - This paper introduces a new fitting approach to allow an efficient part-by-part reconstruction or update of editable CAD models fitting the point cloud of a digitized... 相似文献
10.
Osmar Franca Siebel Marcio Cherem Schneider Carlos Galup-Montoro 《Microelectronics Journal》2012,43(5):329-336
This paper exploits a universal current-based definition of the threshold voltage (VT) and discusses some direct methods to measure it. The consistency, accuracy, and sensitivity of the extraction procedures to second-order effects are examined through numerical simulations and experimental measurements. In addition to three procedures based on dc current measurements we propose an automatic VT-extractor circuit which allows the direct determination of the threshold voltage with minimum influence of second-order effects. 相似文献