CYP154C5 Regioselectivity in Steroid Hydroxylation Explored by Substrate Modifications and Protein Engineering**

CYP154C5 from Nocardia farcinica is a P450 monooxygenase able to hydroxylate a range of steroids with high regio- and stereoselectivity at the 16α-position. Using protein engineering and substrate modifications based on the crystal structure of CYP154C5, an altered regioselectivity of the enzyme in steroid hydroxylation had been achieved. Thus, conversion of progesterone by mutant CYP154C5 F92A resulted in formation of the corresponding 21-hydroxylated product 11-deoxycorticosterone in addition to 16α-hydroxylation. Using MD simulation, this altered regioselectivity appeared to result from an alternative binding mode of the steroid in the active site of mutant F92A. MD simulation further suggested that the entrance of water to the active site caused higher uncoupling in this mutant. Moreover, exclusive 15α-hydroxylation was observed for wild-type CYP154C5 in the conversion of 5α-androstan-3-one, lacking an oxy-functional group at C17. Overall, our data give valuable insight into the structure–function relationship of this cytochrome P450 monooxygenase for steroid hydroxylation.     

Lung lamellar bodies lack certain key enzymes of phospholipid metabolism

Palmitoyl CoA-glycerol-3-phosphate acyltransferase, phosphatidate phosphohydrolase, and phospholipase A were assayed in subcellular fractions of rat lung, including lamellar bodies, the putative site of storage and secretion of lung surfactant. The specific activity of each of these enzymes in lamellar bodies was relatively low and could be entirely accounted for by a small contamination of the lamellar bodies fraction by microsomes, as quantitated by the presence of the microsomal marker reduced triphosphopyridine nucleotide cytochromec reductase. These data indicate that lamellar bodies are not the site of synthesis of the lipid component of pulmonary surfactant by pathways involving these enzymes.     

Suction-based Grasp Point Estimation in Cluttered Environment for Robotic Manipulator Using Deep Learning-based Affordance Map

Machine Intelligence Research - Perception and manipulation tasks for robotic manipulators involving highly-cluttered objects have become increasingly in-demand for achieving a more efficient...     

Ontology-driven geographic information integration: A survey of current approaches

Integrating different information sources is a growing research area within different application domains. This is particularly true for the geographic information domain which is facing new challenges because newer and better technologies are capturing large amounts of information about the Earth. This trend can be combined with increases in the distribution of GIS (Geographic Information Systems) on the Web, which is leading to the proliferation of different geospatial information repositories and the subsequent need to integrate information across repositories to get consistent information. To overcome this situation, many proposals use ontologies in the integration process. In this paper we analyze and compare the most widely referred proposals of geographic information integration, focusing on those using ontologies as semantic tools to represent the sources, and to facilitate the integration process.     

Fuzzy geographically weighted clustering using artificial bee colony: An efficient geo-demographic analysis algorithm and applications to the analysis of crime behavior in population

Geo-demographic analysis is an essential part of a geographical information system (GIS) for predicting people’s behavior based on statistical models and their residential location. Fuzzy Geographically Weighted Clustering (FGWC) serves as one of the most efficient algorithms in geo-demographic analysis. Despite being an effective algorithm, FGWC is sensitive to initialize when the random selection of cluster centers makes the iterative process falling into the local optimal solution easily. Artificial Bee Colony (ABC), one of the most popular meta-heuristic algorithms, can be regarded as the tool to achieve global optimization solutions. This research aims to propose a novel geo-demographic analysis algorithm that integrates FGWC to the optimization scheme of ABC for improving geo-demographic clustering accuracy. Experimental results on various datasets show that the clustering quality of the proposed algorithm called FGWC-ABC is better than those of other relevant methods. The proposed algorithm is also applied to a decision-making application for analyzing crime behavior problem in the population using the US communities and crime dataset. It provides fuzzy rules to determine the violent crime rate in terms of linguistic labels from socioeconomic variables. These results are significant to make predictions of further US violent crime rate and to facilitate appropriate decisions on prevention such the situations in the future.     

Those Who are no Longer: Photographic image and representation of women’s absence(s) in Mayra Martell’s Ensayo de la Identidad at Ciudad Juarez

The article analyses the work of Mexican photographer Mayra Martell — particularly her series Ensayo de la identidad. Desaparición de mujeres en Ciudad Juárez, México 2005–2010 (Identity Essay. Disappearance of women in Ciudad Juarez, Mexico 2005–2010) — focusing on the visual strategies chosen to represent the absence of those women. this work is particularly interesting, considering both its points of contact and its distance from the strategies used for the representation of desaparecidos (victims of forced disappearance) in contemporary Argentine photography. Special attention is paid to the visual device proposed by Martell to represent these absent bodies and the strategies chosen to denounce these increasingly occurring individual stories with two biographical elements in common: being female and being young. Further, the relationships with previous productions from the Argentine photographic context are discussed in order to reflect on the specific procedures that can induce a tension on the naturalized link between photography and memory, between absence and representation, and between image and denunciation.     

The influence of calcination temperature on catalytic activities in a Co based catalyst for CO2 dry reforming

The carbon dioxide dry reforming of methane (CDR) reaction could be thermodynamically favored in the range of 800 to 1,000 °C. However, the catalyst in this reaction should be avoided at the calcination temperature over 800 °C since strong metal support interaction (SMSI) in this temperature range can decrease activity due to loss of active sites. Therefore, we focused on optimizing the temperature of pretreatment and a comparison of surface characterization results for CDR. Results related to metal sintering over support, re-dispersion by changing of particle size of metal-support, and strong metal support interaction were observed and confirmed in this work. In our conclusion, optimum calcination temperature for a preparation of catalyst was proposed that 400 °C showed a higher and more stable catalytic activity without changing of support characteristics.     

The nature of polymer grafts and substrate shape on the surface degradation of poly(l‐lactide)

Surface grafting of functional polymers is an effective method to alter material properties and degradation behavior. Two different substrate shapes of poly(l ‐lactide) (PLLA), i.e., films and microparticles, were surface‐grafted with hydrophilic monomers, and their surface degradation was monitored. Surface grafting with a hydrophilic and acidic polymer graft [acrylic acid (AA)] induced large alterations in the surface morphology and topography of the films. In contrast, hydrophilic and neutral polymer grafts [acrylamide (AAm)] had no significant effect on the surface degradation behavior, while the PLLA reference and co‐monomeric (AA/AAm) polymer‐grafted samples exhibited intermediate surface degradation rates. The grafted PAA chains induced a local acidic environment on the surface of the substrates, which in turn catalyzed the surface degradation process. This effect was more pronounced in the films than in the microparticles. Thus, the nature of the grafted chains and substrate geometry were shown to affect the surface degradation behavior of PLLA substrates. © 2015 The Authors Journal of Applied Polymer Science Published by Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132, 42736.     

Evaluation of Effective Width and Distribution Factors for GFRP Bridge Decks Supported on Steel Girders

Glass fiber-reinforced polymer (GFRP) bridge deck systems offer an attractive alternative to concrete decks, particularly for bridge rehabilitation projects. Current design practice treats GFRP deck systems in a manner similar to concrete decks, but the results of this study indicate that this approach may lead to nonconservative bridge girder designs. Results from a number of in situ load tests of three steel girder bridges having the same GFRP deck system are used to determine the degree of composite action that may be developed and the transverse distribution of wheel loads that may be assumed for such structures. Results from this work indicate that appropriately conservative design values may be found by assuming no composite action between a GFRP deck and steel girder and using the lever rule to determine transverse load distribution. Additionally, when used to replace an existing concrete deck, the lighter GFRP deck will likely result in lower total stresses in the supporting girders, although, due to the decreased effective width and increased distribution factors, the live-load-induced stress range is likely to be increased. Thus, existing fatigue-prone details may become a concern and require additional attention in design.