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排序方式: 共有8378条查询结果,搜索用时 15 毫秒
1.
Bernette Maria Oosterlaken Heiner Friedrich Gijsbertus de With 《Microscopy research and technique》2022,85(1):412-417
Transmission electron microscopy (TEM) is an important analysis technique to visualize (bio)macromolecules and their assemblies, including collagen fibers. Many protocols for TEM sample preparation of collagen involve one or more washing steps to remove excess salts from the dispersion that could hamper analysis when dried on a TEM grid. Such protocols are not standardized and washing times as well as washing solvents vary from procedure to procedure, with each research group typically having their own protocol. Here, we investigate the influence of washing with water, ethanol, but also methanol and 2-propanol, for both mineralized and unmineralized collagen samples via a protocol based on centrifugation. Washing with water maintains the hydrated collagen structure and the characteristic banding pattern can be clearly observed. Conversely, washing with ethanol results in dehydration of the fibrils, often leading to aggregation of the fibers and a less obvious banding pattern, already within 1 min of ethanol exposure. As we show, this process is fully reversible. Similar observations were made for methanol and propanol. Based on these results, a standardized washing protocol for collagenous samples is proposed. 相似文献
2.
Gökelma Mertol Einarsrud Kristian Etienne Tranell Gabriella Friedrich Bernd 《Metallurgical and Materials Transactions B》2020,51(2):850-860
Metallurgical and Materials Transactions B - The quality requirements of aluminum products are steadily increasing, and the presence of non-metallic inclusions have a large impact on the quality of... 相似文献
3.
Kazuo Tsubota Stephen C. Pflugfelder Zuguo Liu Christophe Baudouin Hyo Myung Kim Elisabeth M. Messmer Friedrich Kruse Lingyi Liang Jimena Tatiana Carreno-Galeano Maurizio Rolando Norihiko Yokoi Shigeru Kinoshita Reza Dana 《International journal of molecular sciences》2020,21(23)
Over the past decades, the number of patients with dry eye disease (DED) has increased dramatically. The incidence of DED is higher in Asia than in Europe and North America, suggesting the involvement of cultural or racial factors in DED etiology. Although many definitions of DED have been used, discrepancies exist between the various definitions of dry eye disease (DED) used across the globe. This article presents a clinical consensus on the definition of DED, as formulated in four meetings with global DED experts. The proposed new definition is as follows: “Dry eye is a multifactorial disease characterized by a persistently unstable and/or deficient tear film (TF) causing discomfort and/or visual impairment, accompanied by variable degrees of ocular surface epitheliopathy, inflammation and neurosensory abnormalities.” The key criteria for the diagnosis of DED are unstable TF, inflammation, ocular discomfort and visual impairment. This definition also recommends the assessment of ocular surface epitheliopathy and neurosensory abnormalities in each patient with suspected DED. It is easily applicable in clinical practice and should help practitioners diagnose DED consistently. This consensus definition of DED should also help to guide research and clinical trials that, to date, have been hampered by the lack of an established surrogate endpoint. 相似文献
4.
Vector coupled-mode theory of dielectric waveguides 总被引:1,自引:0,他引:1
A consistent derivation of a system of vector coupled-mode (VCM) equations for parallel dielectric waveguides is presented and compared with earlier versions of the improved coupled-mode theory (ICMT). As a validity test, it is shown that the effectively scalar transverse electric and transverse magnetic (TM) coupled-mode (CM) equations are direct limits of our full VCM formulation. In particular, our formulation does not lead to the fundamental error found with earlier coupled-mode theories (CMTs) in a case of TM fields. Functional equations of our VCMT are consistent with Maxwell's equations and lead to higher precision. They can be applied to complicated arrays of strongly coupled parallel dielectric waveguides with true vectorial behavior. 相似文献
5.
H Neudeck M Joncic C Schuster S Bisson R Hildebrandt T Oney B Stiemer H Hopp R Graf 《Canadian Metallurgical Quarterly》1997,37(6):449-458
Nitric oxide (NO) acts as a modulator of neuronal transmission in mature neuronal systems, including the retina. Recently, NO has also been suggested to have a trophic function during development. We examined immunocytochemically the distribution of NO-producing cells in developing and transplanted rabbit retinas. An antibody detecting the neuronal isoform of its biosynthetic enzyme, nitric oxide synthase (NOS), was used on normal developing retinas [starting at embryonic day (E) 15] and on rabbit retinal transplants after various survival times (1-139 days after surgery). Weakly stained cell bodies were first observed in the proximal margin of the neuroblastic layer at E 29. Stained processes projecting towards a developing inner plexiform layer were also visible at this time point. Immunoreactive cells were located at later stages in the innermost part of the inner nuclear layer and in the ganglion cell layer, and are likely to correspond mainly to amacrine cells. NOS-labelled cells were also found in retinal transplants. The first NOS-labelled cells appeared, as in normal developing retinas, in ages corresponding to E 29 and were still detected in transplants corresponding to postnatal day 123. NOS-labelled cells were seen in areas between rosettes, where amacrine cells are located. NOS-labelled processes were at times seen to project for long distances, forming very distinct plexuses. NOS-containing amacrine cells thus appear both in the transplants and in developing retinas in the embryonic stages, long before synaptic function involving these cells can be expected, suggesting a role for NO not only in neuromodulation but also in retinal development. 相似文献
6.
J. J. Hoetjer und F. Koerts 《Holz als Roh- und Werkstoff》1941,4(1):391-393
Zusammenfassung In Zusammenarbeit von MCN und DSM Holland wurde in den beiden letzten Jahren ein Bestimmungsverfahren ausgearbeitet. Unter
Berücksichtigung des Beladungsgrades und der Luftwechselzahl soll dieses Verfahren zur genauen Erfassung des Formaldehydgehaltes
in der Raumluft von Wohnr?umen (Klimar?umen) führen. Das hier als MCN-Test bezeichnete Verfahren, das sich als für diesen
Zweck geeignet erwiesen hat, wird n?her erl?utert.
Method for determining formaldehyde release from particleboard as related to the concentration of the ambient air
During the past years a method for the determination of formaldehyde release from particleboard has been developed in co-operation of MCN and DSM Holland. Considering the loading factor and the air exchange rate this method shall lead to an exact measurement of formaldehyde content in ambient air of rooms (climate chambers). The method (MCN-test) which proved to be suited for this purpose is explained in detail in this paper.相似文献
7.
M. Pleißner M. Schütz A. Behre und A. Scholl 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1911,22(5):316-327
Ohne Zusammenfassung 相似文献
8.
C. A. Neufeld A. Hasterlik und M. Schütz 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1913,26(2):125-127
Ohne Zusammenfassung 相似文献
9.
10.
G. Reif und H. J. Steinbeck 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1938,76(6):538-543
Ohne ZusammenfassungMitteilung aus dem Chemischen Laboratorium des Reichsgesundheitsamtes. 相似文献