Mannitol and oligosaccharides as new criteria for determining cold tolerance in sugarcane varieties

Sugarcane can be very susceptible to damage by freezes. Freeze-deteriorated cane can cause problems in processing and sometimes leads to a factory shut-down. This study was undertaken during the 2000/2001 harvest season to assess the cold tolerance performance of six commercial sugarcane varieties and to establish new and more sensitive criteria to measure cold tolerance. Two varieties CP 70-321 and CP 79-318, with known cold tolerance, were planted in the study as controls. The other varieties included LHo 83-153, LCP 85-384, HoCP 85-845 and HoCP 91-555. Freezing temperatures occurred on 20 December 2000 when the min. field temperature was −4.4 °C, and again on 21 December, 30 December through 5 January 2001, 9–10 January and 20–21 January. The lowest field temperature recorded was −5.6 °C on 4 January. Freezing conditions prevailed for 8–15 h during each freeze incident. Stalks of all varieties were frozen to the ground following the initial freeze, with freeze cracks evident only after the 4 January freeze. For this study, samples were taken on the date of the first freeze, 20 December, and subsequently again at 7, 14, 22 and 30 days after the first freeze. Criteria used to measure overall stalk cold-tolerance included changes in pH, Brix, dextran (ASI-II method), sucrose, glucose, and fructose concentrations. Mannitol, ethanol and the oligosaccharides, palatinose, leucrose, iso-maltotriose and 1-kestose, were simultaneuously measured using IC-IPAD. Marked differences were observed in most criteria for all varieties, particularly 22 and 30 days after the first freeze. Mannitol was strongly correlated (r²=0.84) with dextran, confirming its use as an indicator for cane dextran deterioration. In comparison, ethanol was only weakly correlated (r²=0.55) with dextran and did not always predict cane dextran deterioration. Iso maltotriose was the most sensitive oligosaccharide indicator of freeze deterioration, although both leucrose and palatinose could be used to confirm whether severe dextran formation (>1500 ppm/Brix) has occurred in cane. Isomaltotriose was strongly correlated (r²=0.89) with dextran and pH (r²=−0.83); pH was also a strong indicator of both dextran (r²=−0.85) and mannitol (r²=−0.92) formation. Four of the varieties, CP 79-318, LCP 85-384, HoCP 85-845 and HoCP 91-555, were shown to be susceptible to other sources of microbial and enzymic deterioration as well as dextran deterioration from Leuconostoc bacteria, especially 30 days after the first freeze. This was indicated by increased glucose/fructose ratios, ethanol formation, changes in 1-kestose concentration, and further sucrose losses.     

Brokers and bricoleurs: entrepreneurship in Wales' online music scene

 The power of some new entrants to the music industry derives from their position as brokers in computer-mediated environments. Brokers act instrumentally to exploit their position within a network which, in turn, depends on their ability to build and sustain links (and, in computer-mediated environments, hyperlinks). Bricolage in computer-mediated entrepreneurship refers to the intuitive manipulation of resources in order to achieve (perhaps tacit) goals. Without careful stewardship of the new intellectual wealth thus created, bricolage may profit neither the individual nor the wider community. Accepted 14 March 2002     

Nanogoldpharmaceutics (i) The use of colloidal gold to treat experimentally-induced arthritis in rat models; (ii) Characterization of the gold in Swarna bhasma, a microparticulate used in traditional Indian medicine

Nanosized gold particles (27 +/− 3 nm) have been proven to be effective in ameliorating the symptoms of mycobacterial-, collagen- and pristane-induced arthritis in rat models. This contrasts with the drug sodium aurothiomalate that was only effective against mycobacterial-induced arthritis but not to the same extent as Au⁰. Gold in the traditional Indian Ayurvedic medicine,Swarna bhasma (gold ash), has been characterized as globular particles of gold with an average size of 56–57 nm.     

Effect of qualitative and quantitative variation in allelochemicals on a generalist insect: Iridoid glycosides and the southern armyworm

The behavioral and physiological effects of plant allelochemicals have been difficult to demonstrate; it is not often clear whether the compounds are deterrent, toxic, or both. In this study, we compared the qualitative and quantitative effects of several iridoid glycosides on a generalist lepidopteran herbivore,Spodoptera eridania (Noctuidae). Larval growth and survivorship and larval preference or avoidance were measured on artificial diets containing different iridoid glycosides at different concentrations. We also tested the toxicity/deterrence of these compounds. We found that iridoid glycosides retarded larval growth significantly at relatively low concentrations and that they were usually avoided in preference tests. The toxicity/ deterrence test did not always reflect the results of these other tests. The merits of using a variety of methods for determining deterrence and/or toxicity of plant allelochemicals are discussed.     

Response of generalist and specialist insects to qualitative allelochemical variation

We examined the effects of a set of four biosynthetically related iridoid glycosides, aucubin, catalpol, loganin, and asperuloside, on larvae of a generalist,Lymantria dispar (Lymantriidae), the gypsy moth, and an adapted specialist, the buckeye,Junonia coenia (Nymphalidae). In general,L. dispar grew and survived significantly less well on artificial diets containing iridoid glycoside, compared to a control diet without iridoid glycosides. In choice tests, previous exposure to a diet containing iridoid glycosides caused larvae subsequently to prefer iridoid glycoside-containing diets even though they were detrimental to growth and survival. In contrast,J coenia larvae grew and survived better on diets with aucubin and catalpol, the two iridoid glycosides found in the host plantPlantago lanceolata (Plantaginaceae), than on diets with no iridoid glycoside or with loganin and asperuloside. The results of choice tests of diets with and without iridoid glycosides and between diets with different iridoid glycosides reflected these differences as well. These results are discussed in terms of (1) differences between generalists and specialists in their response to qualitative variation in plant allelochemical content, (2) the induction of feeding preferences, and (3) the evolution of qualitative allelochemical variation as a plant defense.     

Neuroproteomics and systems biology-based discovery of protein biomarkers for traumatic brain injury and clinical validation

The rapidly growing field of neuroproteomics has expanded to track global proteomic changes underlying various neurological conditions such as traumatic brain injury (TBI), stroke, and Alzheimer's disease. TBI remains a major health problem with approximately 2?million incidents occurring annually in the United States, yet no affective treatment is available despite several clinical trials. The absence of brain injury diagnostic biomarkers was identified as a significant road-block to therapeutic development for brain injury. Recently, the field of neuroproteomics has undertaken major advances in the area of neurotrauma research, where several candidate markers have been identified and are being evaluated for their efficacy as biological biomarkers in the field of TBI. One scope of this review is to evaluate the current status of TBI biomarker discovery using neuroproteomics techniques, and at what stage we are at in their clinical validation. In addition, we will discuss the need for strengthening the role of systems biology and its application to the field of neuroproteomics due to its integral role in establishing a comprehensive understanding of specific brain disorder and brain function in general. Finally, to achieve true clinical input of these neuroproteomic findings, these putative biomarkers should be validated using preclinical and clinical samples and linked to clinical diagnostic assays including ELISA or other high-throughput assays.     

Presheaves as Configured Specifications

The paper addresses a notion of configuring systems, constructing them from specified component parts with specified sharing. This notion is independent of any underlying specification language and has been abstractly identified with the taking of colimits in category theory. Mathematically it is known that these can be expressed by presheaves and the present paper applies this idea to configuration. We interpret the category theory informally as follows. Suppose ? is a category whose objects are interpreted as specifications, and for which each morphism u : X→Y is interpreted as contravariant ‘instance reduction’, reducing instances of specification Y to instances of X. Then a presheaf P: Set ^?op represents a collection of instances that is closed under reduction. We develop an algebraic account of presheaves in which we present configurations by generators (for components) and relations (for shared reducts), and we outline a proposed configuration language based on the techniques. Oriat uses diagrams to express colimits of specifications, and we show that Oriat's category Diag(?) of finite diagrams is equivalent to the category of finitely presented presheaves over ?. Received May 1998 / Accepted in revised form August 2000     

Open-Source Change Logs

A recent editorial in Empirical Software Engineering suggested that open-source software projects offer a great deal of data that can be used for experimentation. These data not only include source code, but also artifacts such as defect reports and update logs. A common type of update log that experimenters may wish to investigate is the ChangeLog, which lists changes and the reasons for which they were made. ChangeLog files are created to support the development of software rather than for the needs of researchers, so questions need to be asked about the limitations of using them to support research. This paper presents evidence that the ChangeLog files provided at three open-source web sites were incomplete. We examined at least three ChangeLog files for each of three different open-source software products, namely, GNUJSP, GCC-g++, and Jikes. We developed a method for counting changes that ensures that, as far as possible, each individual ChangeLog entry is treated as a single change. For each ChangeLog file, we compared the actual changes in the source code to the entries in the ChangeLog> file and discovered significant omissions. For example, using our change-counting method, only 35 of the 93 changes in version 1.11 of Jikes appear in the ChangeLog file—that is, over 62% of the changes were not recorded there. The percentage of omissions we found ranged from 3.7 to 78.6%. These are significant omissions that should be taken into account when using ChangeLog files for research. Before using ChangeLog files as a basis for research into the development and maintenance of open-source software, experimenters should carefully check for omissions and inaccuracies.     

On Logical Foundations of Multilevel Secure Databases

It is envisaged that the application of the multilevel security (MLS) scheme will enhance flexibility and effectiveness of authorization policies in shared enterprise databases and will replace cumbersome authorization enforcement practices through complicated view definitions on a per user basis. However, the critical problem with the current model is that the belief at a higher security level is cluttered with irrelevant or inconsistent data as no mechanism for attenuation is supported. Critics also argue that it is imperative for MLS database users to theorize about the belief of others, perhaps at different security levels, an apparatus that is currently missing and the absence of which is seriously felt.The impetus for our current research is the need to provide an adequate framework for belief reasoning in MLS databases. In this paper, we show that these concepts can be captured in a F-logic style declarative query language, called MultiLog, for MLS deductive databases for which a proof theoretic, model theoretic and fixpoint semantics exist. This development is significant from a database perspective as it now enables us to compute the semantics of MultiLog databases in a bottom-up fashion. We also define a bottom-up procedure to compute unique models of stratified MultiLog databases. Finally, we establish the equivalence of MultiLog's three logical characterizations—model theory, fixpoint theory and proof theory.