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排序方式: 共有348条查询结果,搜索用时 15 毫秒
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Three variable microsatellite loci have been isolated from the American lobster, Homarus americanus. In a population sample from the Gulf of Maine, the effective numbers of alleles (Ne) for the two most variable loci were 16.33 and 13.19, respectively. Reduced variability at all three loci was seen in the European lobster, H. gammarus, for which the maximum Ne was 4.00. The reduction in variability in H. gammarus is consistent with a bottleneck event. Inheritance analysis using H. americanus demonstrated segregation of codominant alleles and the absence of linkage. Null alleles were observed at two loci in inheritance studies. This study demonstrates that microsatellite loci should be useful in studying the population structure of clawed lobsters. 相似文献
3.
Andreas Brodehl Alexey Meshkov Roman Myasnikov Anna Kiseleva Olga Kulikova Brbel Klauke Evgeniia Sotnikova Caroline Stanasiuk Mikhail Divashuk Greta Marie Pohl Maria Kudryavtseva Karin Klingel Brenda Gerull Anastasia Zharikova Jan Gummert Sergey Koretskiy Stephan Schubert Elena Mershina Anna Grtner Polina Pilus Kai Thorsten Laser Valentin Sinitsyn Sergey Boytsov Oxana Drapkina Hendrik Milting 《International journal of molecular sciences》2021,22(7)
About 50% of patients with arrhythmogenic cardiomyopathy (ACM) carry a pathogenic or likely pathogenic mutation in the desmosomal genes. However, there is a significant number of patients without positive familial anamnesis. Therefore, the molecular reasons for ACM in these patients are frequently unknown and a genetic contribution might be underestimated. Here, we used a next-generation sequencing (NGS) approach and in addition single nucleotide polymor-phism (SNP) arrays for the genetic analysis of two independent index patients without familial medical history. Of note, this genetic strategy revealed a homozygous splice site mutation (DSG2–c.378+1G>T) in the first patient and a nonsense mutation (DSG2–p.L772X) in combination with a large deletion in DSG2 in the second one. In conclusion, a recessive inheritance pattern is likely for both cases, which might contribute to the hidden medical history in both families. This is the first report about these novel loss-of-function mutations in DSG2 that have not been previously identi-fied. Therefore, we suggest performing deep genetic analyses using NGS in combination with SNP arrays also for ACM index patients without obvious familial medical history. In the future, this finding might has relevance for the genetic counseling of similar cases. 相似文献
4.
This paper discusses the simulation of bubbling gas-solid flows by using the Eulerian two-fluid approach. Predictions of particle motion, bed expansion, bubble size and bubble velocity in bubbling beds containing Geldart B particles are compared with experimental results and correlations found in the literature. In addition, gas mixing in a bed of Geldart A particles is investigated.An in-house code has been developed based on the finite-volume method and the time-splitting approach using a staggered grid arrangement. The velocities in both phases are obtained by solving the 2D Reynolds-averaged Navier/Stokes equations using a partial elimination algorithm (PEA) and a coupled solver. The k-ε turbulence model is used to describe the turbulent quantities in the continuous phase.In general, the model predictions are in good agreement with experimental data found in the literature. Most important observations are: the level of the restitution coefficient was found to be crucial in order to obtain successful results from 2D axisymmetric simulations of a system containing Geldart B particles. Bubble size and bubble rise velocities are not as sensitive to the restitution coefficient. The turbulence model is of outmost importance concerning gas mixing in a fluidized bed of Geldart A particles.From these numerical analyzes an optimized granular flow two-fluid model can be designed for the purpose of simulating reactive systems in fluidized bed reactors. 相似文献
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Tao Dong Zhaochu Yang Qianhua Su Nhut Minh Tran Eirik Bentzen Egeland Frank Karlsen Yulong Zhang Matteo Joseph Kapiris Henrik Jakobsen 《Microfluidics and nanofluidics》2011,10(4):855-865
This study addresses the need to reduce the risk of clogging when preparing samples for cell concentration, i.e., the CaSki
Cell-lines (epidermoid cervical carcinoma cells). Aiming to develop a non-clogging microconcentrator, we proposed a new counter-flow
concentration unit characterized by the directions of penetrating flows being at an obtuse angle to the main flow, due to
employment of streamlined turbine blade-like micropillars. Based on the optimization results of the counter-flow unit profile,
a fractal arrangement for the counter-flow concentration unit was developed. A counter-flow microconcentrator chip was then
designed and fabricated, with both the processing layer and collecting layer arranged in terms of the honeycomb structure.
Visualized experiments using CaSki cell samples on the microconcentrator chip demonstrated that no cell-clogging phenomena
occurred during the test and that no cells were found in the final filtrate. The test results show an excellent concentration
performance for the microconcentrator chip, while a concentrating ratio of >4 with the flow rate being below 1.0 ml/min. As
only geometrical structure is employed in the passive device, the counter-flow microconcentrator can be easily integrated
into advanced microfluidic systems. Owing to the merit of non-clogging and continuous processing ability, the counter-flow
microconcentrator is not only suitable for the sample preparation within biomedical field, but also applicable in water-particle
separation. 相似文献
7.
Bin Xiao Tao Dong Einar Halvorsen Zhaochu Yang Yulong Zhang Nils Hoivik Dandan Gu Nhut Minh Tran Henrik Jakobsen 《Microsystem Technologies》2011,17(1):115-125
This paper presents the design and fabrication of a micro Pirani gauge using VO
x
as the sensitive material for monitoring the pressure inside a hermetical package for micro bolometer focal plane arrays
(FPAs). The designed Pirani gauge working in heat dissipating mode was intentionally fabricated using standard MEMS processing
which is highly compatible with the FPAs fabrication. The functional layer of the micro Pirani gauge is a VO
x
thin film designed as a 100 × 200 μm pixel, suspended 2 μm above the substrate. By modeling of rarefied gas heat conduction
using the Extended Fourier’s law, finite element analysis is used to investigate the sensitivity of the pressure gauge. Also
the thermal interactions between the micro Pirani gauge and bolometer FPAs are verified. From the fabricated prototype, the
measured device TCR is about −0.8% K−1 and the sensitivity about 1.84 × 10−3 W K−1 mbar−1. 相似文献
8.
Lee Spencer Martin Jakobsen Sunay Shah Graham Cairns 《Journal of the Society for Information Display》2013,21(8):352-360
There has been a rapid increase in the resolution of small‐sized and medium‐sized displays. This study determines an upper discernible limit for display resolution. A range of resolutions varying from 254–1016 PPI were evaluated using simulated display by 49 subjects at 300 mm viewing distance. The results of the study conclusively show that users can discriminate between 339 and 508 PPI and in many cases between 508 and 1016 PPI. 相似文献
9.
Greta Lindwall Karin Frisk Jörgen Andersson Anna Medvedeva Odd Sandberg Ingo Siller 《BHM Berg- und Hüttenm?nnische Monatshefte》2012,157(11):406-413
We present experimental and theoretical comparisons of the coarsening rate for the MX (X?=?C, N or C?+?N) precipitate in five different tool steel grades produced by Powder Metallurgy (PM). The alloys contain different amounts of nitrogen and carbon; ranging from high carbon/low nitrogen to high nitrogen/low carbon. Common for the grades is a high amount of precipitate forming alloying elements resulting in a high fraction of hard phase. Experimentally we have found out that the nitrogen rich precipitates coarsen slower, both for higher and lower carbon content, than the carbon rich precipitates. Coarsening simulations are performed utilizing the DICTRA software and an agreement between calculations and measurements can be concluded. 相似文献
10.