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Chunlong Li Xuefei Du Sheng Tai Xiangyu Zhong Zhidong Wang Zhanliang Hu Lei Zhang Pengcheng Kang Daolin Ji Xingming Jiang Qingxin Zhou Ming Wan Guixing Jiang Yunfu Cui 《International journal of molecular sciences》2014,15(4):6314-6327
To determine the relationships between miR-96-5p/-182-5p and GPC1 in pancreatic cancer (PC), we conducted the population and in vitro studies. We followed 38 pancreatic cancer patients, measured and compared the expression of miR-96-5p/-182-5p, GPC1, characteristics and patients’ survival time of different miR-96-5p/-182-5p expression levels in PC tissues. In an in vitro study, we investigated the proliferation, cycle and apotosis in cells transfected with mimics/inhibitors of the two miRNAs, and determine their effects on GPC1 by dual-luciferase assay. In the follow-up study, we found that the expressions of miR-96-5p/-182-5p were lower/higher in PC tissues; patients with lower/higher levels of miR-96-5p/-182-5p suffered poorer characteristics and decreased survival time. In the in vitro study, the expressions of miR-96-5p/-182-5p were different in cells. Proliferation of cells transfected with miR-96-5p mimics/inhibitors was lower/higher in Panc-1/BxPC-3; when transfected with miR-182-5p mimics/inhibitors, proliferation of cells were higher/lower in AsPC-1/Panc-1. In a cell cycle study, panc-1 cells transfected with miR-96-5p mimics was arrested at G0/G1; BxPC-3 cells transfected with miR-96-5p inhibitors showed a significantly decrease at G0/G1; AsPC-1 cells transfected with miR-182-5p mimics was arrested at S; Panc-1 cells transfected with miR-182-5p inhibitors showed a decrease at S. MiR-96-5p mimics increased the apoptosis rate in Panc-1 cells, and its inhibitors decreased the apoptosis rate in BxPC-3. Dual luciferase assay revealed that GPC1 was regulated by miR-96-5p, not -182-5p. We found that miR-96-5p/-182-5p as good markers for PC; miR-96-5p, rather than -182-5p, inhibits GPC1 to suppress proliferation of PC cells. 相似文献
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通信自适应干扰对消系统的性能分析 总被引:2,自引:0,他引:2
文章对现在的通信自适应干扰对消系统,进行了详细的性能分析,找出了单级的对消系统的对消比不能提高的原因,在此基础上提出了用级联的对消方式来提高对消比。 相似文献
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Quality-aware images. 总被引:4,自引:0,他引:4
Zhou Wang Guixing Wu Hamid Rahim Sheikh Eero P Simoncelli En-Hui Yang Alan Conrad Bovik 《IEEE transactions on image processing》2006,15(6):1680-1689
We propose the concept of quality-aware image, in which certain extracted features of the original (high-quality) image are embedded into the image data as invisible hidden messages. When a distorted version of such an image is received, users can decode the hidden messages and use them to provide an objective measure of the quality of the distorted image. To demonstrate the idea, we build a practical quality-aware image encoding, decoding and quality analysis system, which employs: 1) a novel reduced-reference image quality assessment algorithm based on a statistical model of natural images and 2) a previously developed quantization watermarking-based data hiding technique in the wavelet transform domain. 相似文献
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Identification of tartary buckwheat tea aroma compounds with gas chromatography-mass spectrometry 总被引:2,自引:0,他引:2
Tartary buckwheat tea, which is an important and healthy product, has a distinct malty aroma. However, its characteristic aroma compounds have not been elucidated. The aims of present study were identification and quantification of its aroma compounds. The analyses were performed by gas chromatography-mass spectrometry (GC-MS) after 3 different isolation techniques. Seventy-seven compounds were identified. Among these compounds, 35 were quantified by available standards. The compounds with a high probability of contribution to the tartary buckwheat tea aroma (OAV ≥ 10) were as follows: 2,5-dimethyl-4-hydroxy-3(2H)-furanone, nonanal, 2,3-diethyl-5-methylpyrazine, benzeneacetaldehyde, maltol, 2,5-dimethylpyrazine, 2-ethyl-5-methylpyrazine, trimethylpyrazine. Some nutritional and bioactive compounds were also identified in this study, such as linoleic acid, niacin, vanillic acid, 7-hydroxycoumarin, butylated hydroxytoluene. Practical Application: Tartary buckwheat, one type of buckwheat, has gained much attention from nutritionists and medical doctors in recent years. It is rich in rutin, quercetin, and other nutrients that are good for health. Tartary buckwheat-based product such as tartary buckwheat tea is an important and popular healthy product in China, Japan,South Korea, European countries as well as in American countries. It has a distinct malty aroma. The present study first identified and quantified of its aroma compounds. The results will draw attention to other researchers in food flavor and buckwheat filed. 相似文献
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本文以某高层建筑为例,介绍了首、二层设置商业服务网点的居住建筑改为首、二层为商业的商住楼后,对原有消防水池、消防泵、室内外消火栓管网、自动喷水灭火系统的变更设计。 相似文献
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ATM是一种多服务技术。本文针对ATM层的服务特性进行了详细的分析,重点阐述了各种ATM层服务的定义、业务参数说明及其应用 相似文献