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David Sanz Luis Mata Santiago Cond��n Mar��a ��ngeles Sanz Pedro Razquin 《Food Analytical Methods》2011,4(2):212-220
Concerns regarding the presence of drug residues in foods include allergic reactions, toxicity, technological problems in
fermented products and the development of antibiotic resistance in human pathogens. The analysis of antimicrobial residues
in foods is generally carried out, in a first step, through microbiological screening tests. These tests commonly use Geobacillus stearothermophilus as target specie but show a low ability to detect quinolones. The goal of our study was to evaluate the performance of a
new microbiological test (Equinox) for detection of quinolone residues in muscle. The kit contains an ampoule with a standardized
number of freeze-dried Escherichia coli and must be diluted with a specific detection medium containing a redox indicator. Microbial growth will modify the redox
potential of the medium being observed through a colour change (from blue to brown/orange). Equinox limits of detection for
most of tested quinolones (enrofloxacin, norfloxacin, sarafloxacin, marbofloxacin, ciprofloxacin, danofloxacin and difloxacin)
were below or around maximum residue limit (MRL) UE and CCβ values obtained corresponded with the determined sensitivities.
In contrast, flumequine could not be detected at MRL UE levels. Moreover, Equinox displayed a low sensitivity to other antimicrobials.
Sensitivity data obtained in vitro were consistent when testing incurred muscle samples. Matrix constituents, test batch and
animal species did not affect the performance of the test. Equinox could be easily automated enabling a large numbers of simultaneous
analysis, and a photometric reading can be applied for a precise interpretation. The results obtained in this study prove
that Equinox is a useful tool when screening for quinolone residues or can be combined with other methods for screening of
unknown samples. 相似文献
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Pablo Hernndez‐Alonso Christopher Papandreou Mnica Bull Miguel Ruiz‐Canela Courtney Dennis Amy Deik Dong D. Wang Marta Guasch‐Ferr Edward Yu Estefanía Toledo Cristina Razquin Dolores Corella Ramon Estruch Emilio Ros Montserrat Fit Fernando Ars Miquel Fiol Lluís Serra‐Majem Liming Liang Clary B. Clish Miguel A Martínez‐Gonzlez Frank B Hu Jordi Salas‐Salvad 《Molecular nutrition & food research》2019,63(17)
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Pablo Hernndez‐Alonso Nerea Becerra‐Toms Christopher Papandreou Mnica Bull Marta Guasch‐Ferr Estefanía Toledo Miguel Ruiz‐Canela Clary B. Clish Dolores Corella Courtney Dennis Amy Deik Dong D. Wang Cristina Razquin Jean‐Philippe Drouin‐Chartier Ramon Estruch Emilio Ros Montserrat Fit Fernando Ars Miquel Fiol Lluís Serra‐Majem Liming Liang Miguel A Martínez‐Gonzlez Frank B Hu Jordi Salas‐Salvad 《Molecular nutrition & food research》2020,64(12)
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Patricia Galan-Malo Isabel Mendiara Pedro Razquin 《Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment》2018,35(4):599-604
For many years, the adulteration of milk from sheep, goats or water buffalos with cows’ milk has been a widespread practice due to the higher cost of milk from those other species. Because of this, great concern has been shown by many Protected Designation of Origin councils that have to assure the quality and genuineness of the cheese produced by their associates. Therefore, the whole production chain needs analytical tools that allow the control of potential adulteration. Rapid methods to be used in the field are scarce and have not been validated according to international guidelines. The aim of this work has been to validate a rapid test based on lateral flow immunochromatography to detect cows’ milk in milk from other species, including buffalo’s milk, according to AOAC guidelines. No false-positive result was found after analysing 146 known negative samples from individual animals. The lowest level of adulteration with a Probability of Detection (POD) of 1.00 (confidence interval between 0.94 and 1.00) was found at 0.5% of cows’ milk. This level is below the current EU allowed level of cows’ milk, set at 1%. Variations in the time of assay, volume of the analysis buffer and different batches of the test were evaluated to detect any effect on the false-positive rate or on the limit of detection of the test. The effects of compositional factors (such as high level of fat, protein and somatic cell counts) were also evaluated. The new rapid test to detect cows’ milk in milk from other species is shown to be an adequate tool to control milk quality in routine analysis. This kind of test is very easy to use and it can be performed by untrained staff during milk collection at the farm or upon arrival at dairies. 相似文献
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Obesity is a complex disease that results from the interaction between lifestyle (dietary patterns and sedentary habits) and genetic factors. The recognition of a genetic basis for human obesity has driven to identify putative causal genes to understand the pathways that control body mass and fat deposition in humans as well as to provide personalized treatments and prevention strategies to fight against obesity. More than 120 candidate genes have been associated with obesity-related traits. Genome-wide association study has so far identified over 20 novel loci convincingly associated with adiposity. This review is specifically focused on the study of the effects of melanocortin 4 receptor, Peroxisome proliferator-activated receptor γ and fat mass and obesity associated (FTO) gene variants and their interactions with dietary intake, physical activity or drug administration on body weight control. The advances in this field are expected to open new ways in genome-customized diets for obesity prevention and therapy following personalized approaches. 相似文献
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María del Puy Carretero David Oyarzun Amalia Ortiz Iosu Arizkuren Raul Navas Alejandro García-Alonso 《Mobile Networks and Applications》2008,13(6):627-634
Our research focuses on enabling users to interact with others using 3D avatars with the same appearance and personality in
different media such as the Internet, SMS or television and using different devices such as PCs, MACs, PDAs, mobile phones
and televisions. This work’s main contribution is its use of a unique architecture, tested with a contact application, which
is compatible with different media. The avatar appearance editor, the animation engine and the 3D models are the same for
the different media: TV, SMS and Internet chat. 相似文献
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Karla Alejandra Pérez-Vega Olga Castañer Albert Sanllorente Camille Lassale Emilio Ros Xavier Pintó Ramón Estruch Jordi Salas-Salvadó Dolores Corella Ángel M. Alonso-Gómez Lluis Serra-Majem Cristina Razquin Miquel Fiol José Lapetra Enrique Gómez-Gracia Francisco J. Tinahones Álvaro Hernáez Montserrat Fitó 《Molecular nutrition & food research》2023,67(1):2200338
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