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1.
2.
Hamsters were fed high fiber diets containing cellulose, wheat bran or psyllium. The psyllium was incorporated into high fiber, ready-to-eat (RTE) flakes that were used to formulate the test diet. All the diets contained 0.125% cholesterol. The study was terminated after three weeks. Food intake, weight gain and feed efficiency were not significantly different in the three groups. Serum total and HDL cholesterol levels were reduced significantly by the psyllium diet. Serum triglycerides were 26% lower in the hamsters fed psyllium but because of the large variation the difference did not reach statistical significance. Liver total cholesterol and cholesteryl ester levels were significantly lower in the hamsters fed psyllium. Liver triglycerides were highest in the psyllium-fed hamsters and liver phospholipid levels were similar in the three groups. Liver cholesterol and triglyceride levels were higher in hamsters fed cellulose than in those fed wheat bran. Psyllium formulated into an RTE cereal was effective in reducing serum and liver cholesterol levels in hamsters. 相似文献
3.
Treatment of exponentially growing cultures ofStreptococcus mutans BHT with growth-inhibitory concentrations (0.2 μg/ml) of benzylpenicillin stimulates the incorporation of [2-14C] acetate into lipids excreted by the cells by as much as 69-fold, but does not change the amount of14C incorporated into intracellular lipids. At this concentration of penicillin cellular lysis does not occur. The radioactive
label is incorporated exclusively into the fatty acid moieties of the glycerolipids. The increase in the radioactive content
of the extracellular lipids reflects an actual net increase in the total fatty acid content as determined by a chemical assay.
During a 4-hr incubation in the presence of penicillin, the extracellular fatty acid ester concentration (per mg cell dry
weight) increases 1.5 fold, even though there is no growth or cellular lysis. No change is observed in the intracellular fatty
acid ester content.
An indication of the relative rate of fatty acid synthesis was most readily obtained by placingS. mutans BHT in a buffer containing14C-acetate. Under these nongrowing conditions free fatty acids are the only lipids labeled, a factor which simplifies the assay.
The addition of glycerol to the buffer causes all of the nonesterified fatty acids to be incorporated into glycerolipid. The
cells excrete much of the lipid whether glycerol is present or not. Addition of penicillin to the nongrowth supporting buffer
system does not stimulate the incorporation of [14C]-acetate into fatty acids. However, if cells are exposed to penicillin in a growth-supporting medium and then are transferred
to the nongrowing buffer system containing no penicillin, the previously exposed cells retain the ability to incorporate [14C]-acetate into fatty acid at a higher rate than untreated cells over a prolonged period of time. The stimulation of [14C]-acetate into fatty acids in this system parallels but is not dependent on the stimulation by penicillin of the incorporation
of [14C]-glycerol into glycerolipid and lipoteichoic acid synthesis previously demonstrated by our laboratory.
The material of this paper is part of a thesis to be submitted by J.L.B. in partial fulfillment of the requirements for the
Ph.D. degree from the Department of Biochemistry, Temple University. 相似文献
4.
A parametric investigation of NH4OH catalyzed solvent delignification of poplar was conducted to define pretreatment conditions which would yield an optimal separation of the biomass components and an enzymatic susceptible solid carbohydrate phase. Delignification parameters of interest included concentration of NH4OH, time and temperature of the reaction, and type of solvent. The addition of 0.82 M NH4OH to the delignification liquor increased lignin removal and decreased carbohydrate degradation, but increasing NH4OH concentration had no additional effect. At lower reaction temperatures, the extent of delignification increased with reaction time; at higher temperatures, a “relignification” of the pretreated wood was observed. The delignification and hemicellulose solubilization were modelled and rate constants reported. No major difference between three potential pulping solvents—ethanol, butanol, phenol—was observed. The enzymatic susceptibility of pretreated wood samples was approximately 6-fold greater than that of the untreated poplar. UV absorbance was used to qualitatively characterize the soiubilized lignins. 相似文献
5.
Epidemiological data suggest that populations subsisting on high fiber diets are free of a number of the diseases of Western
civilization, among them coronary heart disease. Studies in animals and man show that each type of fiber exerts it own specific
influence. Thus, in man bran has no effect on serum lipids, but pectin lowers cholesterol levels. In animals fed atherogenic
diets, alfalfa and pectin exert some measure of protection, but cellulose does not. To fully understand the mode of action
of dietary fiber, it is necessary to ascertain the mechanism(s) of action of each chemical component of that fiber. 相似文献
6.
The experiments reported are part of our effort to dissociate the tumor-enhancing effects of dietary fat and high caloric
intake. Rats either were fed ad libitum diets containing 4% corn oil or their calories were restricted by 40% and their diets
contained 13.1% corn oil. Incidence of 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary tumors was 80% in rats fed ad
libitum and 20% in those fed the calorie-restricted diets. Incidence of 1,2-dimethylhydrazine (DMH)-induced colon tumors was
100% in rats fed ad libitum and 53% in those whose caloric intake was restricted by 40%. The tumor yield (tumors per tumor-bearing
rat) was significantly lower in rats on caloric restriction. In another series, rats were fed diets containing 5, 15 or 20%
corn oil ad libitum or were fed calorie-restricted (by 25%) diets which provided 20 or 26.6% corn oil (therefore, the same
absolute amount of fat was consumed in each of the pair-fed groups). Tumor incidence and tumor yield in the two calorie-restricted
groups were similar to those seen in the rats fed 5% fat ad lititum; tumor burden (total g of tumor) was 45–65% lower in the
calorie-restricted rats. The data suggest that caloric intake is a more stringent determinant of tumor growth than fat intake. 相似文献
7.
A method for isolation and quantitation of cellular free fatty acid has been developed. When this method was used to quantitate
the free fatty acid content of various cells and tissues, their levels of free fatty acids were found to vary over a wide
range. In comparing tissue culture cells having different levels of free fatty acid, it was demonstrated that the conditions
of culture and the type of serum in the medium are not responsible for the difference in levels. Isotopic studies have shown
that the cellular free fatty acid is not biosynthesized, but is derived from the free fatty acid of the medium. Preliminary
studies on the fate of the intracellular free fatty acid and a discussion of possible factors controlling the level of this
compound in cells are presented. 相似文献
8.
The inability of states to collect sales taxes from online vendors is a salient issue during the current economic decline. Further, brick and mortar retailers, who must charge customers sales tax, have pushed for legislation requiring online vendors to also do so. States have enacted legislation requiring online vendors, such as Amazon.com LLC, to collect and pay these taxes. In this article the authors describe the issue and make recommendations for resolution. 相似文献
9.
This paper applies the technology acceptance model to explore the digital divide and transformational government (t-government) in the United States. Successful t-government is predicated on citizen adoption and usage of e-government services. The contribution of this research is to enhance our understanding of the factors associated with the usage of e-government services among members of a community on the unfortunate side of the divide. A questionnaire was administered to members, of a techno-disadvantaged public housing community and neighboring households, who partook in training or used the community computer lab. The results indicate that perceived access barriers and perceived ease of use (PEOU) are significantly associated with usage, while perceived usefulness (PU) is not. Among the demographic characteristics, educational level, employment status, and household income all have a significant impact on access barriers and employment is significantly associated with PEOU. Finally, PEOU is significantly related to PU. Overall, the results emphasize that t-government cannot cross the digital divide without accompanying employment programs and programs that enhance citizens’ ease in using such services. 相似文献
10.