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排序方式: 共有75条查询结果,搜索用时 15 毫秒
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Fernando A. Oliveira Neri Alves José A. Giacometti Carlos J. L. Constantino Luiz H. C. Mattoso Ana M. O. A. Balan Aldo E. Job 《应用聚合物科学杂志》2007,106(2):1001-1006
This work describes the preparation and characterization of composite materials obtained by the combination of natural rubber (NR) and carbon black (CB) in different percentages, aiming to improve their mechanical properties, processability, and electrical conductivity, aiming future applications as transducer in pressure sensors. The composites NR/CB were characterized through optical microscopy (OM), DC conductivity, thermal analysis using differential scanning calorimetry (DSC), dynamic mechanical thermal analysis (DMA), thermogravimetry (TGA), and stress–strain test. The electrical conductivity varied between 10?9 and 10 S m?1, depending on the percentage of CB in the composite. Furthermore, a linear (and reversible) dependence of the conductivity on the applied pressure between 0 and 1.6 MPa was observed for the sample with containing 80 wt % of NR and 20% of CB. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci, 2007 相似文献
3.
Marco Albertini Francesca Vallese Marilena Di Valentin Paola Berto Giorgio M. Giacometti Paola Costantini Donatella Carbonera 《International Journal of Hydrogen Energy》2014
[FeFe]-hydrogenases contain a complex [4Fe–4S]-2Fe cluster (H-cluster) and are able to efficiently reduce protons to H2. Due to their potential exploitation for renewable energy production biotechnologies, significant efforts have been put into understanding the mechanisms driving the H-cluster assembly, which involves three conserved proteins. Among them, HydF works as scaffold upon which the H-cluster precursor is synthesized and carrier to deliver it to the hydrogenase, resulting in its activation. A FeS cluster binding sequence (CxHx46-53HCxxC) is conserved in all HydF proteins and should in principle provide four ligands to coordinate the Fe atom. However, we found that alternative metal coordination may exist in different HydF proteins and that only the three cysteines are strictly required, whereas the fourth ligand may vary and is, in any case, readily exchangeable. In this work we analyzed by EPR/HYSCORE the FeS cluster proton environment of HydF from Thermotoga neapolitana to determine the possible role of surrounding residues in the non-cysteinyl iron ligation of the protein. 相似文献
4.
A. Serraino N. Arrigoni F. Ostanello M. Ricchi G. Marchetti P. Bonilauri E. Bonfante F. Giacometti 《Journal of dairy science》2014
Mycobacterium avium ssp. paratuberculosis (MAP) is the etiological agent of paratuberculosis, a chronic contagious bacterial disease primarily affecting dairy cattle. Paratuberculosis represents a dual problem for the milk production chain: in addition to economic losses to affected herds, MAP may have zoonotic potential. Infected herds must be identified in order to implement programs designed to reduce the incidence of disease within and between herds and to prevent MAP from entering the food chain. The objective of this study was to evaluate the sensitivity and specificity of a screening sampling plan (SSP) to detect MAP-positive dairy herds by repetitive analysis of bulk tank milk (BTM) samples by ELISA and in-line milk filter (ILMF) samples by PCR. Samples from BTM and ILMF were collected twice from 569 dairy herds in southern Italy. Additionally, 12,016 individual milk samples were collected: 9,509 from 102 SSP-positive herds (SSP MAP-positive) and 2,507 from 21 randomly selected SSP-negative herds (SSP MAP-negative). There was a total of 126 SSP MAP-positive herds (i.e., 21.3% SSP MAP-positive herds; 95% confidence interval = 18.0–24.9); the within-herd apparent prevalence (AP) ranged between 0.00 and 22.73% (mean 6.07%). A significant difference in within-herd AP was shown between SSP MAP-positive herds and SSP MAP-negative herds. A highly significant association was shown between the median AP herd status (>5%) and positivity to at least one ILMF or BTM sample. The SSP detected a minimum of 56.25% of low AP herds (AP ≤2.0%) up to a maximum of 100% of herds with a within-herd AP ≥8.0%. Overall, the SSP detected 85.57% of herds in which at least one individual milk sample was positive by ELISA. The proposed SSP was an inexpensive and useful tool to detect MAP-positive herds with a higher risk of infection diffusion and milk contamination. Although the SSP cannot be used for MAP-free certification of herds, it could be useful to prioritize appropriate control measures aimed at reducing the prevalence of infection in dairy herds and milk contamination. 相似文献
5.
Ivana Tomaz Luna Maslov Domagoj Stupić Darko Preiner Danijela Ašperger Jasminka Karoglan Kontić 《分离科学与技术》2016,51(2):255-268
Grape skin contains large amounts of different flavonoids so it can be used for their recovery. Optimization of enzyme-assisted extraction of flavonoids was conducted using oenological enzyme preparations with respect to enzyme dosage, temperature, extraction time, pH, and enzyme preparation. Optimal conditions were obtained using enzyme preparation Lallzyme EX-V, at the temperature of 45°C, time of 3 h, pH 2.0, and enzyme dosage of 10.52 mg/g. The new optimized extraction method is less expensive, simple, accurate, and selective for the recovery of simple flavonoids. It is based on an environmentally-friendly extraction solvent which may provide a valuable alternative to conventional methods. 相似文献
6.
D.S Dos Santos Jr.C.R Mendonça D.T Balogh A DhanabalanJ.A Giacometti S.C ZilioO.N Oliveira Jr. 《Polymer》2002,43(16):4385-4390
Aggregation in azopolymers is reported to affect the Langmuir monolayer characteristics and the optically induced birefringence of Langmuir-Blodgett (LB) films from DR19 isophorone polyurethane (PIPDI) and DR19 4,4′ diphenylmethane polyurethane (PMDI). In mixed monolayers with cadmium stearate (CdSt), the folding of PMDI molecules appears to be substantially changed compared to the monolayer of the pure polymer, leading to a surface potential that is higher than observed for monolayers of pure PMDI and of pure CdSt. UV-Vis spectroscopy data of the deposited mixed LB films indicate H-type aggregation for PMDI/CdSt, in contrast to PIPDI/CdSt and other azopolymers investigated earlier. The H-type aggregation precludes photoisomerization, thus requiring a higher laser power for the maximum induced birefringence to be achieved in LB films of PMDI/CdSt. 相似文献
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Vibrations of varying frequency and amplitude are used in many technological areas to control and reduce friction. In this study we report the results of systematic high-precision measurements of the static and sliding friction under the influence of ultrasonic oscillations. We investigate the effect of ultrasonic oscillations for in-plane and out-of-plane oscillations in the completely relevant interval of oscillation amplitudes and sliding velocities and for various material pairings. The experimental results are interpreted on the basis of both macroscopic and microscopic models. There are two main effects which are of interest for tribological applications. Firstly, the frictional force typically decreases with increasing oscillation amplitude, with an oscillation amplitude of about 0.1???m typically being sufficient for a significant decrease of frictional force. Secondly, the decrease of force is larger at smaller sliding velocities; therefore, at sufficiently large oscillation amplitudes, the frictional force always increases with sliding velocity. This effect can be used to suppress frictionally induced vibrations. 相似文献
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10.
Romina Giacometti Florencia Kronberg Ricardo M. Biondi Susana Passeron 《Yeast (Chichester, England)》2009,26(5):273-285
Candida albicans cAMP‐dependent protein kinase (PKA) is coded by two catalytic subunits (TPK1 and TPK2) and one regulatory subunit (BCY1). In this organism the cAMP/PKA signalling pathway mediates basic cellular processes, such as the yeast‐to‐hyphae transition and cell cycle regulation. In the present study, we investigated the role of C. albicans PKA in response to saline, heat and oxidative stresses as well as in glycogen storage. To fine‐tune the analysis, we performed the studies on several C. albicans PKA mutants having heterozygous or homozygous deletions of TPK1 and/or TPK2 in a different BCY1 genetic background. We observed that tpk1Δ/tpk1Δ strains developed a lower tolerance to saline exposure, heat shock and oxidative stress, while wild‐type and tpk2Δ/tpk2Δ mutants were resistant to these stresses, indicating that both isoforms play different roles in the stress response pathway. We also found that regardless of the TPK background, heterozygous and homozygous BCY1 mutants were highly sensitive to heat treatment. Surprisingly, we observed that those strains devoid of one or both TPK1 alleles were defective in glycogen storage, while strains lacking Tpk2 accumulated higher levels of the polysaccharide, indicating that Tpk1 and Tpk2 have opposite roles in carbohydrate metabolism. Copyright © 2009 John Wiley & Sons, Ltd. 相似文献