Enzymatic Synthesis of Glucose- and Xylose Laurate Esters Using Different Acyl Donors,Higher Substrate Concentrations,and Membrane Assisted Solvent Recovery

Glucose- and xylose laurate esters are enzymatically synthesized using equimolar substrate concentrations in 2-methyl-2-butanol, comparing free lauric acid with methyl- and vinyl-laurate as acyl donors. All reactions result in ≥70% acyl donor conversions after 72 h but the activated donors are also partially hydrolyzed to lauric acid, highlighting the difficulty in controlling water presence in this particular reaction system. The esterification of xylose generates a complex product profile, with several regioisomers of monoesters and diesters. The esterification of glucose is quite selective, forming mainly the 6-O monoester (≥96%) with a small presence of two diester isomers (4%). Increasing substrate concentration up to 800 millimoles kg⁻¹ results in lower conversion values (down to 58%) but shows that the reaction proceeds successfully even in the presence of high amounts of insoluble glucose. However, the reaction is less selective and the proportion of diester increases, becoming up to 46% (molar fraction) of the final product. Solvent recovery after esterification can be achieved by organic solvent nanofiltration through a polymeric membrane able to retain ≥80% of all reaction substrates and products. Practical Applications: The use of high substrate concentrations during the enzymatic synthesis of sugar ester biosurfactants leads to product titers that are more industrially appealing, without the need to find a solvent that can solubilize all initial substrate. The sustainability of the enzymatic conversion at mild temperatures can be enhanced by recycling of the reaction solvent through organic solvent nanofiltration, an energy efficient alternative to other traditional methods like distillation.     

Studies on film potentiometric stripping analysis: effects of electrochemical parameters

Film potentiometric stripping analysis (PSA) is a novel method for concentrating the test ion directly on a glassy carbon electrode with subsequent stripping by a chemical oxidant, the redissolution step being followed by a chronopotentiometric sequence. The electrochemical parameters governing both preconcentration and redissolution steps are studied on a rotating disk electrode: experimental results are compared with the theoretical developments recently published. Except for the influence of rde rotation rates on each step and on analytical parameters, experiments and theory are in agreement. Discrepancies concerning the rotation rate effects are studied by potentiostatic coulometry and voltammetry measurements in regard to the preconcentration step: the rotation rate effects are based on the physical behaviour of the rde. Routine analysis is carried out using film PSA, Pb(II) as the test ion and Fe(III) as oxidizing agent, in perchlorate medium.     

Thermal acceptability assessment in buildings located in hot and humid regions in Brazil

The objective of this paper was to perform an analysis on thermal acceptability in naturally ventilated (NVB) and air-conditioned buildings (ACB) located in hot and humid climates in Brazil. Experiments were carried out in April and November 2005 with 1.301 questionnaires based on ISO 10551:1995(E). Indoor and outdoor climatic variables were monitored simultaneously. The results revealed that 53% of the occupants of NVB and 78% of ACB were thermally satisfied. However, some restrictions were observed with the applications of the following methodologies: ISO/FDIS 7730:2005(E); ANSI/ASHRAE Standard 55:2004; Adaptive Temperature Limits (ATG) and prEN15251: 2005(E). Differences were observed between thermal sensation (TSV) and predicted mean vote (PMV) and between the subject's percentages expressing thermal unacceptability of the environment and the PPD calculated according to ISO/FDIS 7730:2005(E).     

Influence of massive heat-pump introduction on the electricity-generation mix and the GHG effect: Comparison between Belgium, France, Germany and The Netherlands

To evaluate the environmental impact of massive heat-pump introduction on greenhouse gas (GHG) emissions in different electricity-generation systems, dynamic simulations have been carried out for four European countries, namely, Belgium, France, Germany and the Netherlands. For this purpose, the simulations are performed with Promix, a tool that models the overall electricity-generation system. Three heating devices are considered for each country, namely classic fossil-fuel heating, heat pumps and electric resistance heating. Both direct heat-pump heating with a coefficient of performance (COP) of 2.5 and accumulation heat-pump heating with a COP of 5 are investigated. The introduction of electric heating in an electricity-generation system increases the demand for electricity and generates a shift of emissions from fossil-fuel heating systems to electrical plants. The results of the simulations reveal that the massive introduction of either heat pump or resistance heating is always favourable to the environment in France. The most environmentally friendly scenario in 2010 is projected to reduce GHG emissions by about 3.8 Mton compared to the reference scenario. In Belgium and Germany, the largest reduction in GHG emissions occurs with accumulation heat pumps. Belgium can save up to 220 kton of GHG emissions, while Germany can attain reductions of 800 kton in 2010. In the Netherlands, a significant reduction can be achieved by considering the addition of gas-fired combined cycle (CC) power plants, together with the introduction of electric heating, resulting in emissions savings of 410 kton.     

Polycyclic N-benzamido imides with potent activity against vaccinia virus

The synthesis and antiviral activity of a series of novel polycyclic analogues of the orthopoxvirus egress inhibitor tecovirimat (ST‐246) is presented. Several of these compounds display sub‐micromolar activity against vaccinia virus, and were more potent than cidofovir (CDV). The more active compounds were about 10‐fold more active than CDV, with minimum cytotoxic concentrations above 100 μM . Chemical manipulations of the two carbon–carbon double bonds present in the compounds were carried out to further explore the structure–activity relationships of these new polycyclic imides. Hydrogenation of the two carbon–carbon double bonds decreases antiviral activity, whereas either cyclopropanation or epoxidation of the double bonds fully eliminates the antiviral activity.     

Aging and the negative priming effect: A meta-analysis.

This article reports results from a meta-analysis on adult age differences in the negative priming effect (21 studies on identity negative priming and 8 on location negative priming). Both younger and older adults were found to be susceptible to the negative priming effect in identity and location tasks. Effect sizes were homogeneous for both tasks, indicating that the data are adequately described without reference to moderator variables. State trace analysis on identity tasks, in which mean latencies in negative priming conditions were regressed onto mean latencies in baseline conditions, showed (a) that in both age groups the negative priming effect is proportional rather than additive and (b) that the negative priming effect is smaller in older adults as compared with younger adults. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Electricity end-uses in the residential sector of Brazil

The residential energy consumption has been studied in many countries as it usually accounts for a large percentage of the total energy consumption. Energy end-uses have also been a matter of concern as they can assist energy system planning. The objective of this paper is to assess the actual scenario of electricity consumption and estimate electricity end-uses in the residential sector of Brazil for different bioclimatic zones. The analysis is based on a survey performed by 17 energy utilities enclosing a total of 17,643 houses or flats over 12 states in Brazil. The survey was performed to obtain electricity consumption data for all household appliances found in houses and flats. The electricity end-uses were estimated by performing weighted averages according to the location of the dwellings in each bioclimatic zone. Results indicate that the largest end-uses are for refrigerator and freezer together, which account for about 38–49% of the electricity consumption in dwellings in Brazil. Air-conditioning and electric shower are the end-uses that are more dependent on the climatic conditions. The main conclusion that can be made from the analysis is that air-conditioning should be a major concern in the residential sector of Brazil in the near future as its ownership is still low, but its electricity consumption is already significant mainly over summer.