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1.
The G1 glycoprotein of California encephalitis (CE) virus plays a critical role in the infection of mosquito and mammalian cells. We found that CE virus enters baby hamster kidney (BHK-21) and Aedes albopictus (C6/36) cells by the endocytic pathway. Ammonium chloride, a lysosomotropic amine that prevents release of virus from endosomes, inhibited infection of both cell types when added within 10 min after viral adsorption. In addition, infected cells formed polykaryons when the extracellular pH was lowered to 6.3; optimal fusion occurred at pH 5.8 and 6.0 (C6/36 and BHK-21 cells, respectively). Two neutralizing G1 MAba, 6D5.5 and 7D4.5, inhibited low pH-induced syncytia formation without affecting viral attachment, suggesting a role for G1 in viral entry. Since viral fusion proteins have been demonstrated to undergo conformational changes at low pH, acid-induced changes in G1 and G2 were assessed. While both G1 and G2 demonstrated low pH-induced alterations in detergent binding, only G1 displayed an altered protease cleavage pattern at the fusion pH. These results indicate that the G1 protein of CE virus undergoes conformational changes necessary for low pH-mediated entry into both mosquito and mammalian cells. 相似文献
2.
PI Lobo C Spencer MT Douglas WC Stevenson TL Pruett 《Canadian Metallurgical Quarterly》1993,55(5):1063-1066
Recent reports indicate a higher incidence of both acute and chronic liver allograft rejection when, at the time of transplantation, the recipients serum contains donor-specific anti-HLA antibodies. From 9/89 to 5/91, 133 liver allografts were performed at our institution. Thirteen liver recipients had donor-specific IgG anti-HLA antibodies (complement-fixing) at the time of transplantation. In eleven patients, antibodies reacted to donor class I antigens while in 1 patient the donor-specific antibody had class II reactivity. Twelve patients have been followed for a minimum of 12 months (median 18 months, range 28-12 months). No hyperacute rejection was seen in any of the cases and four patients had acute rejections. Thus far only one of the twelve patients has biopsy evidence suggestive of chronic liver injury. The remaining have normal liver enzymes and bilirubin. Three of these twelve patients died (one from a myocardial infarction and the others from sepsis) accounting for a one-year graft survival of 75%. There was no significant statistical difference in the one-year graft survival in those recipients without donor-specific antibodies (i.e., 80.5%). In eight of the twelve patients, pretransplant preformed antibody level (PRA) was > 50%. In six of the thirteen patients donor-specific antibody was present at dilutions greater than 1:64. As previously reported, the donor-specific antibody disappeared from the serum posttransplant within hours and did not reappear. In vitro studies demonstrated no factor in portal or hepatic artery blood that could inhibit rabbit complement mediated lysis of anti-HLA antibodies. We conclude that it is not a contraindication to do liver transplants in the presence of donor-specific anti-HLA antibodies. 相似文献
3.
Lima A.M.N. Deep G.S. Neto J.S.R. Freire R.C.S. Lobo P.C. 《IEEE transactions on instrumentation and measurement》1994,43(2):133-138
An identification procedure to estimate the parameters of a thermoresistive solar radiation sensor is presented. The proposed technique employs only electrical excitation for the sensor. The estimation algorithm is recursive and is applied to the sensor model derived from the thermodynamic equilibrium differential equations. The simulation and the experimental results demonstrate the validity of the proposed approach 相似文献
4.
A.c. impedance behaviour of MnO2 and doped -MnO2 electrodes in H2SO4 medium was assessed with a view to explaining the mechanism of the discharge behaviour of MnO2 electrodes in 4m H2SO4 electrolyte. The electrodes used in this work appear to be intermediate cases of planar and porous electrodes as the angles, , made by the low frequency part with the real axis are found to be in the range (30–60°). The Nyquist plots and the Randle plots tend to reinforce the observation made by Tye that the capacity yield is essentially diffusion controlled. The depression and flattening of semi-circles observed reveals a link with the heterogeneity of the planar electrode and with the porosity of the pitted electrode. The deviation from a 45° angle made by the low frequency part with the real axis may either be explained by the roughness of the electrode surface or the shallow pores on the surface of the electrodes; in other words due to the difference between the apparent and true surface areas. The double layer capacitance values of the electrodes seem to subsume adsorption capacitances and diffusion factors. Hence, the relative increase in magnitude. The electrodes appear to behave like planar electrodes when 10 µF is introduced into the circuit as a parallel capacitance since angles 0 vary between 40–58°. The undoped -MnO2 electrode, as well as those prepared from Li-MnO2, Ag-MnO2, and I.C.8, appear to be planar electrodes. 相似文献
5.
MR Harrison GB Mychaliska CT Albanese RW Jennings JA Farrell S Hawgood P Sandberg AH Levine E Lobo RA Filly 《Canadian Metallurgical Quarterly》1998,33(7):1017-22; discussion 1022-3
BACKGROUND/PURPOSE: Fetuses with congenital diaphragmatic hernia (CDH) who have a "poor prognosis" with postnatal treatment now can be identified on the basis of liver herniation, early diagnosis (before 25 weeks' gestation) and a low lung-to-head ratio (LHR). Because complete in utero repair proved unsuccessful for this group, the strategy of temporary tracheal occlusion was developed to gradually enlarge the hypoplastic fetal lung. The purpose of this study is to compare the outcome of patients in the poor-prognosis group treated by one of three methods: (1) standard postnatal care, (2) fetal tracheal occlusion via open hysterotomy, and (3) the recently developed video-fetoscopic (Fetendo) technique of tracheal occlusion without hysterotomy. METHODS: In the past 3 years, 34 of 86 fetuses with an isolated left CDH met criteria for the poor-prognosis group. Thirteen families chose postnatal treatment at an extracorporeal membrane oxygenation (ECMO) center, 13 underwent open fetal tracheal occlusion, and eight underwent fetoscopic tracheal occlusion. RESULTS: The survival rate was 38% in the group treated by standard postnatal therapy, 15% in the open tracheal occlusion group, and 75% in the Fetendo group. There were less postoperative pulmonary complications noted in mothers who underwent the Fetendo procedure versus the open tracheal occlusion. All but one Fetendo clip patient had a striking physiological response demonstrated by sonographic enlargement of the small left lung that was documented postnatally by plain radiographs and its subjective appearance during repair of the CDH. In contrast, only 5 of the 13 open tracheal occlusion patients demonstrated lung growth. CONCLUSION: Fetuses with a left CDH who have liver herniation and a low LHR are at high risk of neonatal demise and appear to benefit from temporary tracheal occlusion when performed fetoscopically, but not when performed by open fetal surgery. 相似文献
6.
Dan Lin Prathima Rao Elisa Bertino Ninghui Li Jorge Lobo 《International Journal of Information Security》2010,9(4):253-273
Policy integration and inter-operation is often a crucial requirement when parties with different access control policies
need to participate in collaborative applications and coalitions. Such requirement is even more difficult to address for dynamic
large-scale collaborations, in which the number of access control policies to analyze and compare can be quite large. An important
step in policy integration and inter-operation is to analyze the similarity of policies. Policy similarity can sometimes also
be a pre-condition for establishing a collaboration, in that a party may enter a collaboration with another party only if
the policies enforced by the other party match or are very close to its own policies. Existing approaches to the problem of
analyzing and comparing access control policies are very limited, in that they only deal with some special cases. By recognizing
that a suitable approach to the policy analysis and comparison requires combining different approaches, we propose in this
paper a comprehensive environment—EXAM. The environment supports various types of analysis query, which we categorize in the
paper. A key component of such environment, on which we focus in the paper, is the policy analyzer able to perform several
types of analysis. Specifically, our policy analyzer combines the advantages of existing MTBDD-based and SAT-solver-based
techniques. Our experimental results, also reported in the paper, demonstrate the efficiency of our analyzer. 相似文献
7.
The ??systematicity argument?? has been used to argue for a classical cognitive architecture (Fodor in The Language of Thought. Harvester Press, London, 1975, Why there still has to be a language of thought? In Psychosemantics, appendix. MIT Press, Cambridge, pp 135?C154, 1987; Fodor and Pylyshyn in Cognition 28:3?C71, 1988; Aizawa in The systematicity arguments. Kluwer Academic Press, Dordrecht, 2003). From the premises that cognition is systematic and that the best/only explanation of systematicity is compositional structure, it concludes that cognition is to be explained in terms of symbols (in a language of thought) and formal rules. The debate, with connectionism, has mostly centered on the second premise-whether an explanation of systematicity requires compositional structure, which neural networks do not to exhibit (for example, Hadley and Hayward, in Minds and Machines, 7:1?C37). In this paper, I will take issue with the first premise. Several arguments will be deployed that show that cognition is not systematic in general; that, in fact, systematicity seems to be related to language. I will argue that it is just verbal minds that are systematic, and they are so because of the structuring role of language in cognition. A dual-process theory of cognition will be defended as the best explanation of the facts. 相似文献
8.
Aligned multi-walled carbon nanotubes (MWCNTs) were produced with different diameters, according to the thickness of Ni catalyst layer, by microwave plasmas enhanced chemical vapor deposition, in N2/H2/CH4 environment. Raman spectroscopy, performed both on top and lateral surfaces, revealed that the relative intensity of the G′ band and the G and G′ bandwidths have high sensitivity to tube diameter distribution and also to structural variations along the tube axis. The D band present a distinct behavior: ID/IG is sensitive to structural organization, particularly along tube axis, while D bandwidth is sensitive to tube diameter distribution. This result may indicate D bandwidth as a parameter to correlate to the diameter of the aligned MWCNTs. 相似文献
9.
Smith P. Shah M. da Vitoria Lobo N. 《Intelligent Transportation Systems, IEEE Transactions on》2003,4(4):205-218
This paper presents a system for analyzing human driver visual attention. The system relies on estimation of global motion and color statistics to robustly track a person's head and facial features. The system is fully automatic, it can initialize automatically, and reinitialize when necessary. The system classifies rotation in all viewing directions, detects eye/mouth occlusion, detects eye blinking and eye closure, and recovers the three dimensional gaze of the eyes. In addition, the system is able to track both through occlusion due to eye blinking, and eye closure, large mouth movement, and also through occlusion due to rotation. Even when the face is fully occluded due to rotation, the system does not break down. Further the system is able to track through yawning, which is a large local mouth motion. Finally, results are presented, and future work on how this system can be used for more advanced driver visual attention monitoring is discussed. 相似文献
10.
Michelle C. Barbosa Nigel R. Messmer Tayra R. Brazil Fernanda R. Marciano Anderson O. Lobo 《Materials science & engineering. C, Materials for biological applications》2013,33(5):2620-2625
Nanohydroxyapatite (nHAp) powders were produced via aqueous precipitation by adopting four different experimental conditions, assisted or non-assisted by ultrasound irradiation (UI). The nHAp powders were characterized by X-ray diffraction, energy-dispersive X-ray fluorescence, Raman and attenuated total reflection Fourier transform infrared spectroscopies, which showed typical surface chemical compositions of nHAp. Analysis found strong connections between UI and the crystallization process, crystal growth properties, as well as correlations between calcination and substitution reactions. 相似文献