全文获取类型
收费全文 | 3221篇 |
免费 | 13篇 |
国内免费 | 13篇 |
专业分类
电工技术 | 17篇 |
综合类 | 12篇 |
化学工业 | 36篇 |
金属工艺 | 21篇 |
机械仪表 | 11篇 |
建筑科学 | 12篇 |
矿业工程 | 1篇 |
能源动力 | 5篇 |
轻工业 | 77篇 |
水利工程 | 2篇 |
石油天然气 | 2篇 |
无线电 | 2篇 |
一般工业技术 | 40篇 |
冶金工业 | 2969篇 |
自动化技术 | 40篇 |
出版年
2023年 | 2篇 |
2022年 | 10篇 |
2021年 | 9篇 |
2020年 | 5篇 |
2019年 | 4篇 |
2018年 | 3篇 |
2017年 | 4篇 |
2015年 | 11篇 |
2014年 | 6篇 |
2013年 | 43篇 |
2012年 | 23篇 |
2011年 | 17篇 |
2010年 | 14篇 |
2009年 | 20篇 |
2008年 | 14篇 |
2007年 | 12篇 |
2006年 | 7篇 |
2005年 | 10篇 |
2004年 | 4篇 |
2003年 | 10篇 |
2001年 | 2篇 |
2000年 | 2篇 |
1999年 | 98篇 |
1998年 | 930篇 |
1997年 | 563篇 |
1996年 | 349篇 |
1995年 | 170篇 |
1994年 | 143篇 |
1993年 | 206篇 |
1992年 | 25篇 |
1991年 | 12篇 |
1990年 | 21篇 |
1989年 | 17篇 |
1988年 | 20篇 |
1987年 | 18篇 |
1986年 | 18篇 |
1985年 | 20篇 |
1983年 | 9篇 |
1982年 | 14篇 |
1981年 | 20篇 |
1980年 | 26篇 |
1979年 | 2篇 |
1978年 | 11篇 |
1977年 | 94篇 |
1976年 | 208篇 |
1975年 | 7篇 |
1973年 | 2篇 |
1964年 | 1篇 |
1955年 | 4篇 |
1954年 | 1篇 |
排序方式: 共有3247条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
5.
The Trichuris muris-mouse model of intestinal helminth infection provides a convenient system to examine the immune mechanisms operating during acute and chronic infection. Particular subsets of helper T lymphocytes (CD4+Th cells) play an important role in regulating infection via the secretion of distinct groups of cytokines. Reciprocal activation of Th cell subsets is associated with either expulsion of the parasites from the intestine (Th2 cells) or chronic infection (Th1 cells). In vivo neutralization experiments using anti-cytokine monoclonal antibodies show that critical cytokines are involved, with interferon-gamma playing an important role in the establishment of chronic trichuriasis and interleukin-4 in expulsion of the parasite from the gut. This model has provided clear evidence of a crucial role for distinct cytokines in mediating host protection against intestinal helminth infection and that manipulation of the immune response through the Th cell-cytokine axis can benefit either the host or the parasite. As such, the T. muris model is poised to generate important new data relevant not only to intestinal helminthiasis but to the wider field of parasite immunity and infection in general. 相似文献
6.
Inhibition of HeLa cell messenger RNA translation by 7-methylguanosine 5'-monophosphate 总被引:2,自引:0,他引:2
LA Weber ER Feman ED Hickey MC Williams C Baglioni 《Canadian Metallurgical Quarterly》1976,251(18):5657-5662
Translation of HeLa cell RNA containing poly(A) in a wheat germ cell-free system is markedly but incompletely inhibited by 7-methylguanosine 5'-monophosphate (m7G5'p). We have analyzed the translation products synthesized in the presence of different concentrations of m7G5'p and find that translation of all mRNAs is equally inhibited. To demonstrate the specificity of the inhibitor for RNAs with 5'-terminal m7G5' ppp... we show that specific translation products of satellite tobacco necrosis virus RNA, which does not have this 5' terminus, are synthesized in the presence of m7G5' p. Protein synthesis programmed by endogenous mRNA in a HeLa cell-free system is inhibited after a 10-min lag by m7G5' p. Other guanosine nucleotides without the 7-methyl group or with the phosphate in a different position are not inhibitor. We show that translation of all mRNAs is inhibited to a similar extent by m7G5'p in the HeLa cell-free system, by synthesizing 35S-labeled proteins in the presence of different inhibitory concentrations of this nucleotide and analyzing the translation products by electrophoresis and autoradiography. Translation of encephalomyocarditis virus RNA added to the HeLa cell-free system is not inhibited by m7"g5p; this viral RNA does not have this nucleotide at the 5' terminus. This indicates that m7G5'p specifically inhibits translation of mRNAs with the 5' terminus m7G5'ppp... and suggests that initiation of translation of picornavirus RNA may proceed via a mechanism different from that of cellular mRNAs. 相似文献
7.
The smoking habits of 56 families who lost babies to the sudden infant death syndrome (SIDS) were compared to those of 86 control families. A higher proportion of SIDS mothers smoked both during pregnancy (61% vs. 42%) and after their babies were born (59% vs. 37%). SIDS mother also smoked a significantly greater number of cigarettes than controls. Exposure to cigarette smoke ("passive smoking") appears to enhance the risk of SIDS for reasons not known. 相似文献
8.
Because of the unresolved controversy regarding the effect of epidural anesthesia upon uterine contractility, it was decided to study its effect on a small number of patients. Intrauterine and intra-arterial continuous pressure, continuous fetal heart rate, and maternal heart rate recordings were obtained from at least 20 minutes before administration of the epidural anesthic until complete dilatation in these patients. Nineteen patients were in spontaneous labor, and 18 had labor stimulated with oxytocin. Plain lidocaine, 1 or 1.5%, was used in 12 patients (30 observations), and lidocaine with epinephrine, 1:200,000 was used in 26 patients (51 observations). Uterine contractions were calculated in Montevideo units for 60 minutes following the epidural anesthetic. The changes, if any, were compared in both groups. There was a significant decrease in uterine activity when epinephrine was added to the anesthetic solution, mainly a lessening of intensity. There were comparable decreases in systolic/diastolic blood pressure in both groups and compensatory tachycardia. In one case, severe hypertension was observed following administration of lidocaine epinephrine. It was concluded that the addition of epinephrine to the anesthetic solution predictably produces diminution of uterine activity, and it does not give "cardiovascular support" to the laboring patient. 相似文献
9.
VA Belitser TV Varetskaia SN Tsynkalovskaia LA Tsariuk LI Shevchenko 《Canadian Metallurgical Quarterly》1976,48(4):521-532
A new diagnostic method to determine fibrinogen and fibrin splitting products is based on the fact that high-molecular fragments acting as specific inhibitors of fibrin self-assembly compose these products. Fibrinogen and fibrin splitting products are detected and determined quantitatively by prolongation of coagulation time in the test with the standard monomeric fibrin. The number of units of anticoagulating activity in the studied urine are the results of the determinations. Sensitivity of the method if necessary may be increased by adding of a certain amount of the fibrinogen and fibrin splitting products to the test that makes a favourable background for the action of the products. The method is rather sensitive, reliable and simple. It may be used in ordinary clinical laboratories. The method proved to be quite satisfactory when testing renal diseases at the clinic. It is of great significance for early detection of the transplanted kidney rejection. The new method is compared with the known immunological method which consist in determination of the inhibitory effect of the fibrinogen and fibrin splitting products on agglutination of erythrocytes of specific antibodies; the erythrocytes being subjected to "tanning" and "loading" with fibrinogen. A satisfactory correlation is found between the results of two methods. 相似文献
10.
Rat adrenal cortex contains a protein(s) that binds pregnenolone with high affinity. This binding, demonstrated by gel filtration and by a dextran-coated charcoal method, was associated with the cytosol and with fractions solubilized by sonication from the mitochondria and microsomes. The binding of pregnenolone was saturable and was inhibited by mercurials and proteolytic enzymes. Pregnenolone-binding was not influenced by the presence of progesterone, deoxycorticosterone, corticosterone or aldosterone, but was inhibited by steroids with a 3beta-hydroxy-5-ene structure similar to pregnenolone, and by hydroxymethylene steroid and cyanoketone. We suggest that this protein is involved in the intracellular transport and retention of pregnenolone within adrenal cortical cells. 相似文献