Interplay between Humoral and CLA+ T Cell Response against Candida albicans in Psoriasis

Candida albicans (CA) infections have been associated with psoriasis onset or disease flares. However, the integrated immune response against this fungus is still poorly characterized in psoriasis. We studied specific immunoglobulins in plasma and the CA response in cocultures of circulating memory CD45RA⁻ cutaneous lymphocyte antigen (CLA)^+/− T cell with autologous epidermal cells from plaque and guttate psoriasis patients (cohort 1, n = 52), and also healthy individuals (n = 17). A complete proteomic profile was also evaluated in plaque psoriasis patients (cohort 2, n = 114) regarding their anti-CA IgA levels. Increased anti-CA IgA and IgG levels are present in the plasma from plaque but not guttate psoriasis compared to healthy controls. CA cellular response is confined to CLA⁺ T cells and is primarily Th17. The levels of anti-CA IgA are directly associated with CLA⁺ Th17 response in plaque psoriasis. Proteomic analysis revealed distinct profiles in psoriasis patients with high anti-CA IgA. C-C motif chemokine ligand 18, chitinase-3-like protein 1 and azurocidin were significantly elevated in the plasma from plaque psoriasis patients with high anti-CA levels and severe disease. Our results indicate a mechanism by which Candida albicans exposure can trigger a clinically relevant IL-17 response in psoriasis. Assessing anti-CA IgA levels may be useful in order to evaluate chronic psoriasis patients.     

Using OGRO and CertiVeR to improve OCSP validation for Grids

Authentication and authorization in many distributed systems rely on the use of cryptographic credentials that in most of the cases have a defined lifetime. This feature mandates the use of mechanisms able to determine whether a particular credential can be trusted at a given moment. This process is commonly named validation. Among available validation mechanisms, the Online Certificate Status Protocol (OCSP) stands out due to its ability to carry near real time certificate status information. Despite its importance for security, OCSP faces considerable challenges in the computational Grid (i.e. Proxy Certificate’s validation) that are being studied at the Global Grid Forum’s CA Operations Work Group (CAOPS-WG). As members of this group, we have implemented an OCSP validation infrastructure for the Globus Toolkit 4, composed of the CertiVeR Validation Service and our Open GRid Ocsp (OGRO) client library, which introduced the Grid Validation Policy. This paper summarizes our experiences on that work and the results obtained up to now. Furthermore we introduce the prevalidation concept, a mechanism analogous to the Authorization Push-Model, capable of improving OCSP validation performance in Grids. This paper also reports the results obtained with OGRO’s prevalidation rules for Grid Services as a proof of concept.

Compositional characteristics and aromatic profile of date palm seeds from seven varieties grown in Tunisia

This paper reports a discrimination study based on the physico‐chemical characteristics, fatty acids and profile of volatile compounds of the seeds from seven date palm varieties (Phoenix dactylifera L.) grown in Tunisia. Date seeds contained 10.49–14.76% moisture; 6.28–11.2% fat (on a dry weight basis); 2.67–12.85% protein; 0.91–6.06% reducing sugar; 0.61–2.98% sucrose and 0.97–1.17% ash. Gas liquid chromatography revealed that the oil fraction of the date palm seeds contained eighteen fatty acids, with oleic acid (30.77–42.50%) and lauric acid (18.51–27.48%) as the main unsaturated and saturated ones. Volatile profile showed differences among varieties. In total, forty‐five compounds were identified, mainly alcohols, aldehydes and unsaturated hydrocarbons ones. This study provides evidence that the seeds of date may be a potential source of valuable nutrients with interesting functionality.     

Structural,Micromechanical and Tribological Characterization of Zn–Ni Coatings: Effect of Sulfate Bath Composition

Zn and Zn–Ni alloy coatings were electrodeposited on mild steel from sulfate-based bath containing Sn as additive. The effect of Ni content on the microstructure, morphology, microhardness and the tribological behavior of these coatings were studied and discussed. Adding Sn in the sulfate bath had a significant effect on the surface morphology, particularly on the Zn–8 wt% Ni coatings. By increasing the Ni concentration from 8 to 14 wt%, the X-ray patterns showed that the phase structure of Zn–Ni alloy coatings was changed from η-phase Ni₃Zn₂₂ to γ-phase Ni₅Zn₂₁. The plastic deformation and delamination were found to be wear mechanisms for the investigated coatings. While the Zn–14 wt% Ni alloys had the best wear resistance, Zn films had the most severe wear volume loss and the highest friction coefficient.     

Stability during storage of LC-PUFA-supplemented infant formula containing single cell oil or egg yolk

The medical community recommends that infant formulae should mimic human milk as far as possible, particularly in regards to long-chain polyunsaturated fatty acids (LC-PUFA). These include arachidonic acid (AA) and docosahexaenoic acid (DHA), both of which provide biochemical and functional benefits to neonates. However, LC-PUFAs are highly susceptible to oxidation and the composition of formulae must be carefully controlled. In this study, the stability of two types of LC-PUFA-supplemented milk-based powdered infant formula was evaluated over the course of 18 months storage at 25 °C and 40 °C. One contained egg yolk phospholipids (IF-EPL) and the other contained triacylglycerides (DHA and AA) synthesized by single cell oils (IF-SCO). The following parameters were monitored: peroxide values, volatile content (propanal, pentanal and hexanal), fatty acid profiles, and potential and free furfural content (5-hydroxymethyl-2-furaldehyde and 2-furaldehyde). In addition, these formulae were subjected to sensory evaluations by a panel of experts. The parameters studied revealed acceptable lipid stability in both types of formula, with better results for IF-EPL. At the end of the study period, significant deficits (p < 0.05) in linoleic acid were noted in both formulae. However, no significant decreases were observed in the other fatty acids, including AA and DHA. In regards to furfural content, both formulae exhibited a similar increase, indicative of the typical Maillard reaction characteristic of products stored for long periods.     

Enhanced Sliding Wear Resistance of Technical Alloys by Hard Particle Reinforcement Using Machine Hammer Peening

Machine hammer peening is a surface treatment technique originally developed for smoothening tools and mold surfaces. Treated surfaces are locally cold-worked, which results in a hardness increase and the induction of compressive residual stresses. In the present work, the feasibility of using this technique as a tool for embedding tungsten carbide hard particles on engineering-relevant substrate materials is systematically investigated. Tungsten carbide particles of three different sizes were embedded onto selected substrates using machine hammer peening. The particle embedment quality of the engineered surfaces was evaluated and correlated to the substrates' mechanical properties. The resulting tribological performance was investigated under reciprocating sliding conditions and the dominant wear mechanisms were correlated with the diameter of the embedded particles. The results show that machine hammer peening is a suitable technique for embedding hard particles in substrates of various materials, which additionally results in an enhancement in wear resistance, thus opening up a wide range of potential applications in tribologically loaded surfaces.     

The future of security in Wireless Multimedia Sensor Networks

This is a position paper on our views on security aspects of Wireless Multimedia Sensor Networks (Secure WMSNs). It is meant to serve as a brief survey. But, more importantly, it gives a perspective on how we foresee the future of this research area, its main challenges, and its future trends. We believe that this paper will spur new discussions and research ideas among the researchers from both the industry and the academic world.     

Extra Virgin Olive Oil Components and Oxidative Stability from Olives Grown in Tunisia

The effects of the contents of lipids, pigments, α-tocopherol and phenols were studied in relation to the antioxidant capacity of five virgin olive oils obtained from five olive cultivars planted in Tunisia (Arbequina, Koroneiki, Leccino, Oueslati and Chemchali). The antioxidant capacities were evaluated by two different radical scavenging activities: radical scavenging activity by the DPPH assay (RSA-DPPH) and total antioxidant status by the ABTS test (TAA-ABTS). The highest contents of antioxidant compounds (75.96, 10.34, 6.32, 15.39 and 241.52 mg kg⁻¹ for oleic acid, O/L ratio, carotenes, chlorophylls and total phenols, respectively) were found for the Koroneiki cultivar except for α-tocopherol and o-diphenols, which had the highest contents (369 and 160.7 mg kg⁻¹, respectively) in the Leccino and Chemchali cultivars (cvs). Furthermore, the highest antioxidant capacity in virgin olive oil was observed in the Koroneiki cultivar (0.24 mmol TE kg⁻¹) followed by the Chemchali and Leccino cvs (0.22 and 0.13 mmol TE kg⁻¹) for the TAA-ABTS test. However, the RSA-DPPH activity was higher for the Chemchali cultivar (19.9%) than for the Koroneiki and Leccino cvs (18.4 and 13.5%, respectively). Correlation between these capacities and the oil composition revealed that they were mainly influenced by the carotene content, followed by chlorophyll and phenolic contents where the ABTS test was more pronounced. Then, the antioxidant capacity of the virgin olive oils was correlated with polar components and the lipid profile which are important for its shelf life.