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排序方式: 共有272条查询结果,搜索用时 15 毫秒
1.
Nitsa Buaron Antonella Mangraviti Francesco Volpin Ann Liu Mariangela Pedone Eric Sankey Dina Aranovich Itay Adar Fausto J. Rodriguez Abraham Nyska Riki Goldbart Tamar Traitel Henry Brem Betty Tyler Joseph Kost 《Advanced functional materials》2021,31(44):2100643
Treating neuroinflammation-related injuries and disorders through manipulation of neuroinflammation functions is being heralded as a new therapeutic strategy. In this study, a novel pectic galactan (PG) polysaccharide based gene therapy approach is developed for targeting reactive gliosis in neuroinflammation. Galectin-3 (Gal-3) is a cell protein with a high affinity to β-galactoside sugars and is highly expressed in reactive gliosis. Since PG carries galactans, it can target reactive gliosis via specific carbohydrate interaction between galactan and Gal-3 on the cell membrane, and therefore can be utilized as a carrier for delivering genes to these cells. The carrier is synthesized by modifying quaternary ammonium groups on the PG. The resulting quaternized PG (QPG) is found to form complexes with plasmid DNA with a mean diameter of 100 nm and have the characteristics required for targeted gene therapy. The complexes efficiently condense large amounts of plasmid per particle and successfully bind to Gal-3. The in vivo study shows that the complexes are biocompatible and safe for administration and can selectively transfect reactive glial cells of an induced cortical lesion. The results confirm that this PG-based delivery system is a promising platform for targeting Gal-3 overexpressing neuroinflammation cells for treating neuroinflammation-related injuries and neurodegenerative diseases. 相似文献
2.
Efficient Simulation of hybrid stripmap/spotlight SAR raw signals from extended scenes 总被引:2,自引:0,他引:2
Franceschetti G. Guida R. Iodice A. Riccio D. Ruello G. 《Geoscience and Remote Sensing, IEEE Transactions on》2004,42(11):2385-2396
The hybrid stripmap/spotlight mode for a synthetic aperture radar (SAR) system is able to generate microwave images with an azimuth resolution better than the one achieved in the stripmap mode and a ground coverage better than the one of the spotlight mode. In this paper, time- and frequency-domain-based procedures to simulate the raw signal in the hybrid stripmap/spotlight mode are presented and compared. We show that a two-dimensional Fourier domain approach, although highly desirable for its efficiency, is not viable. Accordingly, we propose a one-dimensional (1-D) range Fourier domain approach, followed by 1-D azimuth time-domain integration. This method is much more efficient than the time-domain one, so that extended scenes can be considered. In addition, it involves approximations usually acceptable in actual cases. Effectiveness of the simulation scheme is assessed by using numerical examples. 相似文献
3.
4.
The failure pattern of repairable mechanical equipment subject to deterioration phenomena sometimes shows a finite bound for the increasing failure intensity. A non-homogeneous Poisson process with bounded increasing failure intensity is then illustrated and its characteristics are discussed. A Bayesian procedure, based on prior information on model-free quantities, is developed in order to allow technical information on the failure process to be incorporated into the inferential procedure and to improve the inference accuracy. Posterior estimation of the model-free quantities and of other quantities of interest (such as the optimal replacement interval) is provided, as well as prediction on the waiting time to the next failure and on the number of failures in a future time interval is given. Finally, numerical examples are given to illustrate the proposed inferential procedure. 相似文献
5.
采用两种矿产钙质蒙脱土为原料制备了两种层柱分子筛(Al-CLM和Al-CLB)。首先考察了焙烧温度对其比表面积的影响,结果发现在300~500℃范围内,随着焙烧温度的提高,层柱分子筛的比表面积逐渐增大,600℃以后比表面积迅速下降;对负载贵金属Pt或Pd后其比表面积变化情况的研究表明,不论是Al-CLM还是Al-CLB,负载金属后比表面积均下降,且负载Pt后比表面积下降均比负载Pd后比表面积下降得多;在Pt/Al-CLM比表面积因浸渍液的不同而引起的比表面积变化的研究中,发现用酸性较强的浸渍液(H2PtCl6·6H2O)比用酸性较弱的浸渍液(Pt(NH3)4Cl2)所引起的比表面积下降得多,最后在Pt(Pd)/Al-CLM和Pt(Pd)/Al-CLB的苯加氢反应中发现苯转化率随着比表面积的增大而上升。 相似文献
6.
Baroni P. Guida G. Mussi S. 《IEEE transactions on systems, man, and cybernetics. Part A, Systems and humans : a publication of the IEEE Systems, Man, and Cybernetics Society》1998,28(4):394-407
Uncertainty calculus overcomes many of the drawbacks affecting other well-known approaches to uncertain reasoning. Nevertheless, it suffers from other severe limitations and gives rise to counter-intuitive results. We show that common sense inconsistencies of uncertainty calculus originate from a scarce cognitive plausibility in the definition of propagation and aggregation operators, and we propose an improved version of Driankov's uncertainty calculus (1986) where the definition of such operators is substantially revised according to common sense criteria. Revised uncertainty calculus allows overcoming in a general and uniform way all the problems identified in uncertainty calculus and provides a substantial enhancement of cognitive plausibility. It has been successfully tested in the design and development of a real application concerning preventive diagnosis of power transformers 相似文献
7.
Simone Carpanzano Mariangela Santorsola nf-core community Francesco Lescai 《International journal of molecular sciences》2022,23(23)
Horizontal gene transfer (HGT) is well described in prokaryotes: it plays a crucial role in evolution, and has functional consequences in insects and plants. However, less is known about HGT in humans. Studies have reported bacterial integrations in cancer patients, and microbial sequences have been detected in data from well-known human sequencing projects. Few of the existing tools for investigating HGT are highly automated. Thanks to the adoption of Nextflow for life sciences workflows, and to the standards and best practices curated by communities such as nf-core, fully automated, portable, and scalable pipelines can now be developed. Here we present nf-core/hgtseq to facilitate the analysis of HGT from sequencing data in different organisms. We showcase its performance by analysing six exome datasets from five mammals. Hgtseq can be run seamlessly in any computing environment and accepts data generated by existing exome and whole-genome sequencing projects; this will enable researchers to expand their analyses into this area. Fundamental questions are still open about the mechanisms and the extent or role of horizontal gene transfer: by releasing hgtseq we provide a standardised tool which will enable a systematic investigation of this phenomenon, thus paving the way for a better understanding of HGT. 相似文献
8.
Maria Addolorata Bonifacio Riccardo Laterza Angela Vinella Annalisa Schirinzi Mariangela Defilippis Francesca Di Serio Angelo Ostuni Antonio Fasanella Maria Addolorata Mariggi 《International journal of molecular sciences》2022,23(17)
Serological assays are useful in investigating the development of humoral immunity against SARS-CoV-2 in the context of epidemiological studies focusing on the spread of protective immunity. The plaque reduction neutralization test (PRNT) is the gold standard method to assess the titer of protective antibodies in serum samples. However, to provide a result, the PRNT requires several days, skilled operators, and biosafety level 3 laboratories. Therefore, alternative methods are being assessed to establish a relationship between their outcomes and PRNT results. In this work, four different immunoassays (Roche Elecsys® Anti SARS-CoV-2 S, Snibe MAGLUMI® SARS-CoV-2 S-RBD IgG, Snibe MAGLUMI® 2019-nCoV IgG, and EUROIMMUN® SARS-CoV-2 NeutraLISA assays, respectively) have been performed on individuals healed after SARS-CoV-2 infection. The correlation between each assay and the reference method has been explored through linear regression modeling, as well as through the calculation of Pearson’s and Spearman’s coefficients. Furthermore, the ability of serological tests to discriminate samples with high titers of neutralizing antibodies (>160) has been assessed by ROC curve analyses, Cohen’s Kappa coefficient, and positive predictive agreement. The EUROIMMUN® NeutraLISA assay displayed the best correlation with PRNT results (Pearson and Spearman coefficients equal to 0.660 and 0.784, respectively), as well as the ROC curve with the highest accuracy, sensitivity, and specificity (0.857, 0.889, and 0.829, respectively). 相似文献
9.
Maria Infantino Aris Tsalouchos Edda Russo Selene Laudicina Valentina Grossi Barbara Lari Maurizio Benucci Lorenzo Stacchini Amedeo Amedei Patrizia Casprini Danilo Villalta Pietro Claudio Dattolo Mariangela Manfredi 《International journal of molecular sciences》2022,23(20)
The vulnerable population of kidney transplant recipients (KTRs) are low responders to COVID-19 vaccines, so specific immune surveillance is needed. The interferon-gamma (IFN-γ) release assay (IGRA) is effective in assessing T cell-mediated immunity. We assessed SARS-CoV-2-directed T cell responses in KTRs with absent antibody production after a third dose of the mRNA-1273 vaccine, using two different IGRAs. A cohort of 57 KTRs, who were actively followed up, received a third dose of the mRNA-1273 vaccine. After the evaluation of humoral immunity to SARS-CoV-2, 14 seronegative patients were tested with two commercial IGRAs (SD Biosensor and Euroimmun). Out of 14 patients, one and three samples were positive by IGRAs with Euroimmun and SD Biosensor, respectively. The overall agreement between the two assays was 85.7% (κ = 0.444). In addition, multivariate linear regression analysis showed no statistically significant association between the IFN-γ concentration, and the independent variables analyzed (age, gender, years since transplant, total lymphocytes cells/mcl, CD3+ cells/mcl, CD3+ CD4+ cells/mcl, CD3+ CD8+ cells/mcl, CD19+ cells/mcl, CD3-CD16+CD56+ cells/mcl) (p > 0.01). In a vulnerable setting, assessing cellular immune response to complement the humoral response may be advantageous. Since the two commercial IGRAs showed a good agreement on negative samples, the three discordant samples highlight the need for further investigations. 相似文献
10.
Ombretta Repetto Laura Caggiari Mariangela De Zorzi Caterina Elia Lara Mussolin Salvatore Buffardi Marta Pillon Paola Muggeo Tommaso Casini Agostino Steffan Christine Mauz-Krholz Maurizio Mascarin Valli De Re 《International journal of molecular sciences》2022,23(17)
Classical pediatric Hodgkin Lymphoma (HL) is a rare malignancy. Therapeutic regimens for its management may be optimized by establishing treatment response early on. The aim of this study was to identify plasma protein biomarkers enabling the prediction of relapse in pediatric/adolescent HL patients treated under the pediatric EuroNet-PHL-C2 trial. We used untargeted liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomics at the time of diagnosis—before any therapy—as semiquantitative method to profile plasma proteins specifically associated with relapse in 42 children with nodular sclerosing HL. In both the exploratory and the validation cohorts, six proteins (apolipoprotein E, C4b-binding protein α chain, clusterin, fibrinogen γ chain, prothrombin, and vitronectin) were more abundant in the plasma of patients whose HL relapsed (|fold change| ≥ 1.2, p < 0.05, Student’s t-test). Predicting protein function with the Gene Ontology classification model, the proteins were included in four biological processes (p < 0.01). Using immunoblotting and Luminex assays, we validated two of these candidate biomarkers—C4b-binding protein α chain and clusterin—linked to innate immune response function (GO:0045087). This study identified C4b-binding protein α chain and clusterin as candidate early plasma biomarkers of HL relapse, and important for the purpose of shedding light on the molecular scenario associated with immune response in patients treated under the EuroNet-PHL-C2 trial. 相似文献