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排序方式: 共有1030条查询结果,搜索用时 15 毫秒
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Joo H. Kang Eujin Um Alexander Diaz Harry Driscoll Melissa J. Rodas Karel Domansky Alexander L. Watters Michael Super Howard A. Stone Donald E. Ingber 《Small (Weinheim an der Bergstrasse, Germany)》2015,11(42):5657-5666
Magnetic nanoparticles have been employed to capture pathogens for many biological applications; however, optimal particle sizes have been determined empirically in specific capturing protocols. Here, a theoretical model that simulates capture of bacteria is described and used to calculate bacterial collision frequencies and magnetophoretic properties for a range of particle sizes. The model predicts that particles with a diameter of 460 nm should produce optimal separation of bacteria in buffer flowing at 1 L h−1. Validating the predictive power of the model, Staphylococcus aureus is separated from buffer and blood flowing through magnetic capture devices using six different sizes of magnetic particles. Experimental magnetic separation in buffer conditions confirms that particles with a diameter closest to the predicted optimal particle size provide the most effective capture. Modeling the capturing process in plasma and blood by introducing empirical constants (ce), which integrate the interfering effects of biological components on the binding kinetics of magnetic beads to bacteria, smaller beads with 50 nm diameters are predicted that exhibit maximum magnetic separation of bacteria from blood and experimentally validated this trend. The predictive power of the model suggests its utility for the future design of magnetic separation for diagnostic and therapeutic applications. 相似文献
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Joshua C. Price Simon J. Levett Valentin Radu David A. Simpson Aina Mogas Barcons Christopher F. Adams Melissa L. Mather 《Small (Weinheim an der Bergstrasse, Germany)》2019,15(22)
Fluorescent nanodiamonds (fNDs) containing nitrogen vacancy (NV) centers are promising candidates for quantum sensing in biological environments. This work describes the fabrication and implementation of electrospun poly lactic‐co‐glycolic acid (PLGA) nanofibers embedded with fNDs for optical quantum sensing in an environment, which recapitulates the nanoscale architecture and topography of the cell niche. A protocol that produces uniformly dispersed fNDs within electrospun nanofibers is demonstrated and the resulting fibers are characterized using fluorescent microscopy and scanning electron microscopy (SEM). Optically detected magnetic resonance (ODMR) and longitudinal spin relaxometry results for fNDs and embedded fNDs are compared. A new approach for fast detection of time varying magnetic fields external to the fND embedded nanofibers is demonstrated. ODMR spectra are successfully acquired from a culture of live differentiated neural stem cells functioning as a connected neural network grown on fND embedded nanofibers. This work advances the current state of the art in quantum sensing by providing a versatile sensing platform that can be tailored to produce physiological‐like cell niches to replicate biologically relevant growth environments and fast measurement protocols for the detection of co‐ordinated endogenous signals from clinically relevant populations of electrically active neuronal circuits. 相似文献
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Melissa Kurtz 《Journal of Failure Analysis and Prevention》2007,7(3):175-178
Fuel system assemblies used on recreational watercraft failed after a short time in service in a seawater environment. Examination
of the assemblies revealed significant degradation of the polyacetal connectors of the wire subassemblies. Optical and microscopic
evaluations indicated the complete loss of connector material implying decomposition of the resin, along with mud-cracking
and a rough surface morphology signifying chemical attack. An elemental analysis performed on both an intact connector and
degraded connector suggested a potential chemical agent in the form of zinc chloride. At elevated temperatures, concentrated
zinc chloride solutions are known to decompose polyacetal resins. The source of the zinc chloride was established as corrosion
products that resulted during the dezincification of the internal brass connector through exposure to saltwater in the application. 相似文献
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Discusses common presenting complaints and those of emergent significance that have an organic etiology. The following categories are discussed: head, eyes, ears, throat, neck, cardiac, respiratory, gastrointestinal, genito-urinary, musculoskeletal, and neurologic complaints. It is suggested that many therapists lack adequate medical information to suspect the presence of organic problems when symptoms are indistinguishable from psychogenic/psychosomatic problems. (4 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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The present study examined whether siblings experience marital conflict differently and whether such differences, if present, were associated with differences in their adjustment. Self-report data about marital conflict, children's depressed mood, behavioral conduct, and externalizing problems were obtained from 122 sibling pairs (mean ages = 10 and 12 years) and their parents. Results indicated that siblings were significantly different in exposure to and appraisals of marital conflict. Differences in siblings' exposure to marital conflict were significantly correlated with differences in their depressed mood, behavioral conduct, and externalizing problems. Differences in siblings' feelings of self-blame for marital conflict were significantly correlated with differences in their depressed mood and behavioral conduct. Children who experienced more marital conflict than their siblings had more adjustment problems than their siblings. These results highlight the importance of studying siblings' unique experiences of marital conflict to better understand its impact on children's adjustment. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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Children can express thoughts in gesture that they do not express in speech--they produce gesture-speech mismatches. Moreover, children who produce mismatches on a given task are particularly ready to learn that task. Gesture, then, is a tool that researchers can use to predict who will profit from instruction. But is gesture also useful to adults who must decide how to instruct a particular child? We asked 8 adults to instruct 38 third- and fourth-grade children individually in a math problem. We found that the adults offered more variable instruction to children who produced mismatches than to children who produced no mismatches--more different types of instructional strategies and more instructions that contained two different strategies, one in speech and the other in gesture. The children thus appeared to be shaping their own learning environments just by moving their hands. Gesture not only reflects a child's understanding but can play a role in eliciting input that could shape that understanding. As such, it may be part of the mechanism of cognitive change. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献