Exopolysaccharide from Pantoea sp. BCCS 001 GH isolated from nectarine fruit: production in submerged culture and preliminary physicochemical characterizations

Food Science and Biotechnology - Exopolysaccharide (EPS), as potential microbial base polysaccharide source, has plenty of applications due to its unique physicochemical structure. A Pantoea sp....     

PCR amplification of 18S rRNA,single cell protein production and fatty acid evaluation of some naturally isolated microalgae

Microalgae were isolated during a screening program from soil samples collected from paddy-fields of Fars province, south of Iran. The protein content was assayed by the Kochert method. Total genomic DNA were isolated and used for PCR amplification of the 18S rRNA gene. The sequences were determined for 12 species of microalgae. Some bioinformatic tools were used for more investigation on these biologic data. Total lipids from five microalgal species were extracted and used for determination of different types of fatty acids by gas chromatography–mass spectrometry method. In our experiments the green algae yielded a maximum protein of about 42% ± 1.64. The DNA sequences were published in the NCBI under specific accession numbers. The composition of fatty acids was mainly, myristic acid, palmitic acid, oleic acid, α-linolenic acid, and γ-linolenic acid.