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排序方式: 共有203条查询结果,搜索用时 15 毫秒
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Synergistic Effect of PVDF-Coated PCL-TCP Scaffolds and Pulsed Electromagnetic Field on Osteogenesis
Yibing Dong Luvita Suryani Xinran Zhou Padmalosini Muthukumaran Moumita Rakshit Fengrui Yang Feng Wen Ammar Mansoor Hassanbhai Kaushik Parida Daniel T. Simon Donata Iandolo Pooi See Lee Kee Woei Ng Swee Hin Teoh 《International journal of molecular sciences》2021,22(12)
Bone exhibits piezoelectric properties. Thus, electrical stimulations such as pulsed electromagnetic fields (PEMFs) and stimuli-responsive piezoelectric properties of scaffolds have been investigated separately to evaluate their efficacy in supporting osteogenesis. However, current understanding of cells responding under the combined influence of PEMF and piezoelectric properties in scaffolds is still lacking. Therefore, in this study, we fabricated piezoelectric scaffolds by functionalization of polycaprolactone-tricalcium phosphate (PCL-TCP) films with a polyvinylidene fluoride (PVDF) coating that is self-polarized by a modified breath-figure technique. The osteoinductive properties of these PVDF-coated PCL-TCP films on MC3T3-E1 cells were studied under the stimulation of PEMF. Piezoelectric and ferroelectric characterization demonstrated that scaffolds with piezoelectric coefficient d33 = −1.2 pC/N were obtained at a powder dissolution temperature of 100 °C and coating relative humidity (RH) of 56%. DNA quantification showed that cell proliferation was significantly enhanced by PEMF as low as 0.6 mT and 50 Hz. Hydroxyapatite staining showed that cell mineralization was significantly enhanced by incorporation of PVDF coating. Gene expression study showed that the combination of PEMF and PVDF coating promoted late osteogenic gene expression marker most significantly. Collectively, our results suggest that the synergistic effects of PEMF and piezoelectric scaffolds on osteogenesis provide a promising alternative strategy for electrically augmented osteoinduction. The piezoelectric response of PVDF by PEMF, which could provide mechanical strain, is particularly interesting as it could deliver local mechanical stimulation to osteogenic cells using PEMF. 相似文献
4.
Uniprocessor schedulability theory made great strides, in part, due to the simplicity of composing the delay of a job from
the execution times of higher-priority jobs that preempt it. In this paper, we bound the end-to-end delay of a job in a multistage
pipeline as a function of job execution times on different stages under preemptive as well as non-preemptive scheduling. We
show that the end-to-end delay is bounded by that of a single virtual “bottleneck” stage plus a small additive component. This contribution effectively transforms the pipeline into a
single stage system. The wealth of schedulability analysis techniques derived for uniprocessors can then be applied to decide
the schedulability of the pipeline. The transformation does not require imposing artificial per-stage deadlines, but rather
models the pipeline as a whole and uses the end-to-end deadlines directly in the single-stage analysis. It also does not make
assumptions on job arrival patterns or periodicity and thus can be applied to periodic and aperiodic tasks alike. We show
through simulations that this approach outperforms previous pipeline schedulability tests except for very short pipelines
or when deadlines are sufficiently large. The reason lies in the way we account for execution overlap among stages. We discuss
how previous approaches account for overlap and point out interesting differences that lead to different performance advantages
in different cases. Further, we also show that in certain cases non-preemptive scheduling can result in higher system utilization
than preemptive scheduling in pipelined systems. We hope that the pipeline delay composition rule, derived in this paper,
may be a step towards a general schedulability analysis foundation for large distributed systems.
相似文献
Tarek AbdelzaherEmail: |
5.
Ravi Shanker Vidyarthy Dheerendra Kumar Dwivedi Vasudevan Muthukumaran 《Materials and Manufacturing Processes》2018,33(7):709-717
In the present work, the influence of process parameters such as welding current (I), welding speed (S), and flux coating density (F) on different aspects of weld bead geometry for example depth of penetration (DOP), bead width (BW), depth to width ratio (D/W), and weld fusion zone area (WA) were investigated by using the central composite design (CCD). 9–12% Cr ferritic stainless steel (FSS) plates were welded using A-TIG welding. It was observed that all input variables have a direct influence on the DOP, BW, and D/W. However, flux coating density has no significant effect on WA. Mathematical models were generated from the obtained responses to predict the weld bead geometry. An optimized DOP, BW, D/W, and WA of 6.95?mm, 8.76?mm, 0.80, and 41.99?mm2, respectively, were predicted at the welding current of 213.78 A, the welding speed of 96.22?mm/min, and the flux coating density of 1.99?mg/cm2. Conformity test was done to check the practicability of the developed models. The conformity test results were in good agreement with the predicted values. Arc constriction and reversal in Marangoni convection were considered as major mechanisms for the deep and narrow weld bead during A-TIG welding. 相似文献
6.
Po-Ying Yeh Nicholas A. A. Rossi Jayachandran N. Kizhakkedathu Mu Chiao 《Microfluidics and nanofluidics》2010,9(2-3):199-209
A poly(dimethylsiloxane) (PDMS)-based functional microfluidic device containing a charged matrix of PDMS pillar arrays grafted with hyperbranched polyglycerols (HPGs) was developed. Samples of PDMS were modified with allylamine plasma to form amine groups on the surface prior to the covalent grafting of succinimdyl ester-functionalized HPGs. The anionic functionality of the PDMS channel matrices was developed by altering the number of carboxyl groups present on the HPGs. The grafting of HPGs onto PDMS plates was investigated via contact angle measurement and attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), while the grafting of the inside channel was investigated by electroosmotic flow (EOF) measurements. The charge density on grafted HPG was optimized to minimize the nonspecific protein adsorption and increase the selective capture of positively charged proteins. A proof-of-concept device was fabricated on PDMS and demonstrated that the device selectively captures positively charged protein (avidin) from a mixture of bovine serum albumin (BSA)-avidin at pH 7.4 in phosphate buffered saline (PBS). In order to increase the capture efficiency of the proteins in this PDMS-based device, pillar arrays have been fabricated within the channel. As a demonstration, the new device separated two proteins with an avidin capture efficiency of 100 ± 2.95% per 3 min from a 0.02 mg/ml protein solution (avidin:BSA wt ratio: 1:1). This new microfluidic-based device shows a great deal of promise as a tool for protein capture and analysis. 相似文献
7.
This article presents a novel method to improve the measurement sensitivity and reduce impact forces in tapping-mode atomic force microscopy by reshaping the tip trajectory. A tapping drive signal composed of two harmonics is used to generate an oscillating trajectory with a broader valley compared to the typical sinusoidal trajectory. The wide broad valley reduces the velocity of the tip in the vicinity of the sample and allots a greater portion of each period in the vicinity of the sample. Numerical simulations show that this results in decreased impact force and increased sensitivity of the cantilever oscillation to changes in tip-sample offset. Experimental results demonstrate an increase in image sharpness and decrease in tip wear using the bi-harmonic driving signal. 相似文献
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Abundant improvements have occurred in materials handling, especially in metal joining. Pulsed current gas tungsten arc welding (PCGTAW) is one of the consequential fusion techniques. In this work, PCGTAW of AISI 4135 steel engendered through powder metallurgy (P/M) has been executed, and the process parameters have been highlighted applying Taguchi’s L9 orthogonal array. The results show that the peak current (Ip), gas flow rate (GFR), welding speed (WS) and base current (Ib) are the critical constraints in strong determinant of the Tensile strength (TS) as well as percentage of elongation (% Elong) of the joint. The practical impact of applying Genetic algorithm (GA) and Simulated annealing (SA) to PCGTAW process has been authenticated by means of calculating the deviation between predicted and experimental welding process parameters. 相似文献
10.