Microbiological contamination of fresh-cut produce in Korea

Food Science and Biotechnology - This study evaluated the microbiological contamination of fresh-cut produce in Korea. A total of 108 fresh-cut vegetables and fruits were surveyed for the aerobic...     

γ-aminobutyric acid production in skim milk co-fermented with Lactobacillus brevis 877G and Lactobacillus sakei 795

γ-Aminobutyric acid (GABA)-producing Lactobacillus brevis 877G strain isolated from traditional Korean fermented food, kimchi, produced 18.94 mM GABA under 59.13 mM L-monosodium glutamate (MSG). Also, skim milk co-fermented with Lb. brevis 877G as a GABA producer and Lactobacillus sakei 795 as a protease producer resulted in a significant increase in GABA production (22.51 mM) with 29.57 mM MSG. The results indicate that Lb. brevis 877G itself and skim milk cofermented with Lb. brevis 877G and Lb. sakei 795 could be developed to be a useful starter for functional fermented food and dairy food product fortified in GABA.     

Biosynthesis of glyceride glycoside (nonionic surfactant) by amylosucrase,a powerful glycosyltransferase

Amylosucrase (ASase, E.C. 2.4.1.4) is a powerful transglycosylation enzyme that can transfer glucose from sucrose to the hydroxyl (-OH) group of various compounds. In this study, recombinant ASases from Deinococcus geothermalis (DgAS) and Bifidobacterium thermophilum (BtAS) were used to synthesize biosurfactants based on the computational analysis of predicted docking simulations. Successful predictions of the binding affinities, conformations, and three-dimensional structures of three surfactants were computed from receptor-ligand binding modes. DgAS and BtAS were effective in the synthesis of biosurfactants from glyceryl caprylate, glyceryl caprate, and polyglyceryl-2 caprate. The results of the transglycosylation reaction were consistent for both ASases, with glyceryl caprylate acceptor showing the highest concentration, as confirmed by thin layer chromatography. Furthermore, the transglycosylation reactions of DgAS were more effective than those of BtAS. Among the three substrates, glyceryl caprylate glycoside and glyceryl caprate glycoside were successfully purified by liquid chromatography–mass spectrometry (LC–MS) with the corresponding molecular weights.     

Antimicrobial activities of leptospermone isolated from Leptospermum scoparium seeds and structure–activity relationships of its derivatives against foodborne bacteria

Food Science and Biotechnology - This study was carried out to determine the antimicrobial activities of leptospermone isolated from Leptospermum scoparium and its derivatives against six foodborne...     

Linarin down-regulates phagocytosis,pro-inflammatory cytokine production,and activation marker expression in RAW264.7 macrophages

Food Science and Biotechnology - Plant-extracted flavonoid glycosides have been reported to be bioactive compounds with pleiotropic functions, including antioxidant, anti-inflammatory, and...     

Preparation of Copper Oxide with High Surface Area Associated with Mesoporous Silica

Copper oxide was synthesized by the one-pot synthesis methods associated with mesoporous silica in different synthetic conditions. The SBA route with Cu citrate sol was the most effective to prepare CuO with high surface area, where its crystallite size could be controlled with the pore size of the mesoporous structure. It also showed better catalytic activity for toluene oxidation.     

Hepatoprotective effect of fermented rice bran against carbon tetrachloride-induced toxicity in mice

The protective effect of fermented rice bran (GF) against carbon tetrachloride (CCl₄)-induced hepatotoxicity in mice was investigated. Hepatic fibrosis was induced via long-term oral administration of CCl₄. GF was added to feed. Mice sera were analyzed 8 weeks after administration. The alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, both markers of hepatic injury, were reduced in the GF group. Total cholesterol (TC) and triglycerides (TG) levels were also reduced. The glutathione (GSH) level, an antioxidant in liver tissue, increased. Collagen is major component of the extracellular matrix (ECM). Persistent liver damage causes excess remodeling of liver tissue and ECM deposition, causing liver fibrosis. Liver injury was reduced and the amount of collagen, which is synthesized during recovery from liver injury, was significantly reduced in GF fed mice. Thus, the liver was protected against toxic chemicals by GF in mice with CCl₄-induced hepatic fibrosis with an increased antioxidative reaction.     

Whole-cell bioconversion using non-Leloir transglycosylation reactions: a review

Food Science and Biotechnology - Microbial biocatalysts are evolving technological tools for glycosylation research in food, feed and pharmaceuticals. Advances in bioengineered Leloir and...     

Probiotic and antioxidant properties of C30 carotenoid-producing Lactiplantibacillus plantarum isolated from kimchi

Food Science and Biotechnology - This study focuses on the development of functional probiotics using caroteonid-producing lactic acid bacteria (LAB) with antioxidant properties. Thirty LAB strains...