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Angel B. Encarnacion Fernand Fagutao Orapint Jintasataporn Wanchai Worawattanamateekul Ikuo Hirono Toshiaki Ohshima 《Food research international (Ottawa, Ont.)》2012,45(1):232-237
This report investigated the melanosis-inhibiting properties of crude water soluble extract from the base and fruiting body waste of an edible mushroom (Flammulina velutipes) containing 9.13 ± 2.03 mg/mL 2-thiol-l-histidine-betaine (ergothioneine, ESH). Immersion of live full-grown black tiger shrimp (Penaeus monodon) and Pacific white shrimp (Litopenaeus vannamei) in a 0.5% w/v solution of mushroom extract for 1 h significantly decreased polyphenoloxidase (PPO) activity in shrimp hemolymph and expression of the prophenoloxidase (proPO) gene in hemocytes. Consequently, the development of melanosis in the treated shrimp during ice storage was prevented. Treatment with a 0.05% w/v solution of sodium sulfite and 4-hexyl-1,3-benzenediol had a similar effect. Enzyme assays showed that ESH is a non-competitive inhibitor. It is proposed that ESH possibly interacts directly with Cu2+ at the putative binding sites of PPO and proPO, based on copper-chelating activity analyses, thus preventing melanosis in the shrimp. This study indicated that application of ESH-rich F. velutipes mushroom extract from trimming waste is an effective natural alternative to synthetic melanosis-inhibiting agents to prevent postmortem melanosis in shrimp. 相似文献
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