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PROTECTIVE EFFECT OF LYSOSTAPHIN FROM STAPHYLOCOCCUS SIMULANS AGAINST GROWTH OF STAPHYLOCOCCUS AUREUS IN MILK AND SOME OTHER FOOD PRODUCTS 总被引:4,自引:0,他引:4
The effect of lysostaphin from Staphylococcus simulans expressed in Escherichia coli TOP10 strain on Staphylococcus aureus used for inoculation of milk, ground pork and mayonnaise salad was investigated. The populations of this pathogen in ultrahigh‐temperature milk preserved at 4C by lysostaphin added up to concentrations of 1.5 or 3.0 µg/mL were reduced by 0.73 and 0.92 log(cfu/mL) in control samples without enzyme addition. The protective influence of lysostaphin was diminished in case of milk storage (20C) prolonged up to 24 h. Furthermore, a final reduction level by 0.92 log(cfu/mL) was achieved after 24 h of pork storage. The smaller and more dependent on enzyme concentration inactivation of S. aureus was observed in the case of the mayonnaise salad, and it led to the conclusion that some food components or proteolytic enzymes originating from other bacteria caused lysostaphin inactivation. 相似文献
3.
The AOCS official method for the determination of free fatty acids (FFAs) is used for monitoring the extent of deterioration in deep-frying oils. While it is accurate and reliable it is far too subjective and time-consuming as it depends on the identification of a clear end point. The VERI-FRI PRO FFA-75 quick test proved to be a fast and reliable alternative procedure for measuring FFAs and, in addition, was far more environmentally friendly. 相似文献
4.
The frying stability of a regular soybean oil (RSO) was compared with a modified low linolenic-acid soybean oil (LLSO). The rate of free fatty acid (FFA) formation was similar for both oils during 72 h of frying. Despite a reduction in polyunsaturated fatty acids, however, the rate of formation of total polar compounds (TPC) in LLSO was greater than RSO. The higher production of TPCs in LLSO was explained, in part, by a lower level of tocopherols and a more rapid rate of degradation of the main isomers, γ- and δ tocopherols. This study points to the limitations of predicting frying stability based solely on fatty acid composition. 相似文献
5.
MARK S. ROSENTRAUB DAVID SWINDELL MICHAEL PRZYBYLSKI DANIEL R. MULLINS 《Journal of Urban Affairs》1994,16(3):221-239
ABSTRACT: Many policy analysts have cautioned against public spending for professional and amateur sports. Within the last year, numerous cities have received demands from major and minor league teams for investments. These investments by the public sector can involve hundreds of millions of dollars and are usually defended by the economic impact of the facilities or teams and the economic development and revitalization which will follow. Indianapolis formulated an economic development strategy which relied substantially on sports. In addition, its development policies did not involve one team or facility, but a series of investments. As a result, the policies followed in Indianapolis afford an opportunity to measure the ability of sport facilities to encourage other investments and enhance economic development. The results reported here indicate that a sports strategy, even one as pronounced and as articulated as that of Indianapolis, is likely to have an inconsequential impact on development and economic growth. 相似文献
6.
BRONISLAW DROZDOWSKI ZDZISLAWA HAZUKA ROMAN PAWLOWICZ MARIA TYNEK 《Journal of Food Lipids》1999,6(3):205-213
During deep fat frying a series of undesirable transformations occur. These include oxidation, polymerization, and hydrolysis. Use of additives to reduce the access of air to the frying oil may be one of the preventive methods. Such additives reduce the amount of foam formed. In this work, polydimethyl siloxane (PDMS) at 3 ppm was added to the frying rapeseed oil for frying potato fritters. It was found that oxidation was significantly limited as it reduced peroxide, anisidine, E1% lcm values and oxidized triacylglycerol (TAG) contents. Polymerization was also limited as a reduction in the contents of TAG polymer and dimer occurred. Addition of PDMS also reduced degradation of polyenoic acids of the frying oil. Oil without PDMS was less resistant to these transformations. 相似文献
7.
This paper presents a simple procedure for the approximate minimization of multiple-valued functions using multiple-valued decision trees. The proposed procedure is compared with a near-absolute procedure, using for the test four-valued functions of four variables. The results show a great advantage for our procedure with respect to the CPU time needed. 相似文献
8.
This paper discusses the introduction of a computer-algebra system to perform visualizations in mathematics education.With theIllustrated Mathematicsproject, we provide a comprehensive collection of graphics and animations for various topics in mathematics, which can directly be used for teaching. Because the programs (written inMathematica) we used for the creation of this collection are included, it is easy to design new examples by modifying parameters in existing examples. Therefore,Illustrated Mathematicscan be used as a first step of the introduction of a computer-algebra system in mathematics education.In the second part, we report on the development of a workbench and learning environment, with which the students can discover the mathematical concepts by themselves. For that, the given examples have to be didactically enriched and processed. By creating their own visualizations of increasing complexity, the students improve their knowledge about the underlying computer-algebra system. 相似文献
9.
ANNA M. SANTIAGO GEORGE C. GALSTER ANGELA A. KAISER ANA H. SANTIAGO‐SAN ROMAN REBECCA A. GRACE ANDREW T. W. LINN 《Journal of Urban Affairs》2010,32(2):171-198
ABSTRACT: Questions have been raised about the wisdom of low‐income homeownership policies for many reasons. One potential reason to be skeptical: low‐income homebuyers perhaps may be constrained to purchase homes in disadvantaged neighborhoods. This is a potential problem because home purchases in such neighborhoods: (1) may limit appreciation; (2) may reduce quality of life for adults; and (3) may militate against reputed advantages of homeownership for children. Our study examines the neighborhood conditions of a group of 126 low‐income homebuyers who purchased their first home with assistance from the Home Ownership Program (HOP) operated by the Denver Housing Authority. Our approach is distinguished by its use of a comprehensive set of objective and subjective indicators measuring the neighborhood quality of pre‐move and post‐move neighborhoods. Do low‐income homebuyers sacrifice neighborhood quality to buy their homes? Our results suggest that the answer to this question is more complex than it might at first appear. On the one hand, HOP homebuyers purchased in a wide variety of city and suburban neighborhoods. Nonetheless, a variety of neighborhood quality indicators suggest that these neighborhoods, on average, were indeed inferior to those of Denver homeowners overall and to those in the same ethnic group. However, our analyses also revealed that their post‐move neighborhoods were superior to the ones they lived in prior to homeownership. Moreover, very few HOP destination neighborhoods evinced severe physical, environmental, infrastructural, or socioeconomic problems, as measured by a wide variety of objective indicators or by the homebuyers' own perceptions. Indeed, only 10% of HOP homebuyers perceived that their new neighborhoods were worse than their prior ones, and only 8% held pessimistic expectations about their new neighborhoods' quality of life. Finally, we found that Black homebuyers fared less well than their Latino counterparts, on average, in both objective and subjective measures. 相似文献
10.
We present an electronic system that calculates the discrete Walsh transform of a function of which any number of samples has been previously taken. In previous publications, one finds systems designed for a fixed number of samples, normally a power of two. This restriction is too great bearing in mind that no physical function is really periodical, especially in the field of biophysics, where -many important applications of this type of device are found. This makes it impossible to obtain a preset number of samples and makes the selection of sampling frequency difficult. 相似文献