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Cho-Yi Chen Masaoki Kawasumi Tien-Yun Lan Chi-Lam Poon Yi-Sian Lin Pin-Jou Wu Yao-Chung Chen Bing-Hong Chen Cheng-Hsien Wu Jeng-Fan Lo Rueyhung Roc Weng Yi-Chen Sun Kai-Feng Hung 《International journal of molecular sciences》2021,22(1)
Endoplasmic reticulum (ER) stress response is an adaptive program to cope with cellular stress that disturbs the function and homeostasis of ER, which commonly occurs during cancer progression to late stage. Late-stage cancers, mostly requiring chemotherapy, often develop treatment resistance. Chemoresistance has been linked to ER stress response; however, most of the evidence has come from studies that correlate the expression of stress markers with poor prognosis or demonstrate proapoptosis by the knockdown of stress-responsive genes. Since ER stress in cancers usually persists and is essentially not induced by genetic manipulations, we used low doses of ER stress inducers at levels that allowed cell adaptation to occur in order to investigate the effect of stress response on chemoresistance. We found that prolonged tolerable ER stress promotes mesenchymal–epithelial transition, slows cell-cycle progression, and delays the S-phase exit. Consequently, cisplatin-induced apoptosis was significantly decreased in stress-adapted cells, implying their acquisition of cisplatin resistance. Molecularly, we found that proliferating cell nuclear antigen (PCNA) ubiquitination and the expression of polymerase η, the main polymerase responsible for translesion synthesis across cisplatin-DNA damage, were up-regulated in ER stress-adaptive cells, and their enhanced cisplatin resistance was abrogated by the knockout of polymerase η. We also found that a fraction of p53 in stress-adapted cells was translocated to the nucleus, and that these cells exhibited a significant decline in the level of cisplatin-DNA damage. Consistently, we showed that the nuclear p53 coincided with strong positivity of glucose-related protein 78 (GRP78) on immunostaining of clinical biopsies, and the cisplatin-based chemotherapy was less effective for patients with high levels of ER stress. Taken together, this study uncovers that adaptation to ER stress enhances DNA repair and damage tolerance, with which stressed cells gain resistance to chemotherapeutics. 相似文献
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Size has been related with pea seed growth and maturation [1], and it is also considered an important parameter when peas are chosen for canning [2]. Some changes occur in the composition of vegetables during blanching, which are the partial loss of major components [3], that are further reduced during canning, mainly due to leaching into the water used for processing [4]. This leaching can be significant if the brine is discarded and not used as a part of the food consumed, as it is often done with most of canned vegetables [5]. Pea seed is an important source of protein for humans, and it has been suggested [6] that when considering pea seeds protein quality more emphasis should be given to the study of the albumin fraction, because it is the fraction rich in the sulphur amino acids. Although electrophoretic analysis of heat-treated proteins can be considered as time consuming it has been used to monitor changes in specific proteins and mixtures of protein subjected to a variety of heat treatments [7]. In general, heat treatments result in a drastic change in polyacrylamide gel electrophoresis (PAGE) patterns in the presence of the detergent sodium dodecyl sulfate (SDS) by reducing the number of bands compared to untreated samples [8]. The purpose of this study was to monitor changes in the albumin fraction of the protein of 4 sizes of peas in the raw, blanched and canned products, and the can brine solutions, using SDS-PAGE. 相似文献
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Renato Cataluña Rosângela da Silva Eliana Weber de Menezes Ricardo Boeira Ivanov 《Fuel》2008,87(15-16):3362-3368
Oxygenated fuels increase fuel consumption due to their low enthalpy of combustion; however, their high antiknock index renders them suitable for use in engines with a high compression rate, increasing their thermal yield. This study evaluated the performance of biorenewable oxygenated fuels (ethanol and isoamyl alcohol) and partially renewable fuels (ETBE: ethyl tert-butyl ether, TAEE: tert-amyl ethyl ether and di-TAE: di-tert-amyl ether) with high degree of purity and in mixtures with automotive gasoline, based on tests with Otto cycle engines. Among the oxygenated fuels evaluated here, di-TAE was found to present the best characteristics of performance, both individually and in mixtures with gasoline. 相似文献
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In this study, waste biological sludge is converted to an adsorbent by chemical activation with sulphuric acid. The adsorbent obtained is then applied to the aerated vessel of an activated sludge process treating glucose and phenol to improve the quality of the treated effluent. The sludge-based carbonaceous adsorbent was found to be mesoporous in nature, with a good adsorption capacity for large molecular weight compounds and limited removal efficiency for smaller molecules such as phenol. The addition of carbon, either sludge-based or commercial, enhanced phenol removal from 58% to 98.7% and from 87% to 93% the organic matter removal as measured by the chemical oxygen demand (COD) when operated with feed concentrations of 100 mg phenol/l and 2500 mg COD/l. No differences were found between the activated sludge-activated carbon bench scale continuous reactors operating with either commercial or sludge-based adsorbents in spite of the higher adsorption capacity of the former. It is suggested that powdered adsorbent bioregeneration in the combined AS-PAC system may be impaired by the obstruction of pores due to bacterial growth, the effect being more important for the commercial activated carbon with a narrower pore size distribution. 相似文献
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Pablo Guzmán Javier Díaz Jarno Ralli Rodrigo Agís Eduardo Ros 《Machine Vision and Applications》2014,25(3):699-711
The automotive industry invests substantial amounts of money in driver-security and driver-assistance systems. We propose an overtaking detection system based on visual motion cues that combines feature extraction, optical flow, solid-objects segmentation and geometry filtering, working with a low-cost compact architecture based on one focal plane and an on-chip embedded processor. The processing is divided into two stages: firstly analog processing on the focal plane processor dedicated to image conditioning and relevant image-structure selection, and secondly, vehicle tracking and warning-signal generation by optical flow, using a simple digital microcontroller. Our model can detect an approaching vehicle (multiple-lane overtaking scenarios) and warn the driver about the risk of changing lanes. Thanks to the use of tightly coupled analog and digital processors, the system is able to perform this complex task in real time with very constrained computing resources. The proposed method has been validated with a sequence of more than 15,000 frames (90 overtaking maneuvers) and is effective under different traffic situations, as well as weather and illumination conditions. 相似文献
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Enrique J. Fernandez-Sanchez Leonardo Rubio Javier Diaz Eduardo Ros 《Machine Vision and Applications》2014,25(5):1211-1225
Background subtraction consists of segmenting objects in movement in a video captured by a static camera. This is typically performed using color information, but it leads to wrong estimations due to perspective and illumination issues. We show that multimodal approaches based on the integrated use of color and depth cues produce more accurate and robust results than using either data source independently. Depth is less affected by issues such as shadows or foreground objects similar to background. However, objects close to the background may not be detected when using only range information, being color information complementary in those cases. We propose an extension of a well-known background subtraction technique which fuses range and color information, as well as a post-processing mask fusion stage to get the best of each feature. We have evaluated the method proposed using a well-defined dataset and different disparity estimation algorithms, showing the benefits of our method for fusion color and depth cues. 相似文献
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