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1.
A protein phosphatase was purified from the stroma of Pea (Pisum sativum L.) chloroplasts that is capable of dephosphorylating synthetic phosphopeptides. Following chromatographic purification of greater than 400-fold, two-dimensional electrophoresis indicated that the stromal protein phosphatase is a 29-kD protein. A similar molecular size was determined for the protein-phosphatase activity using gel-permeation chromatography, indicating that the stromal protein phosphatase is probably a monomer. The purified enzyme was able to dephosphorylate synthetic phosphopeptides, which mimic the thylakoid light-harvesting complex II (LHC-II) N terminus, as well as LHC-II in thylakoid membranes, but did not dephosphorylate the major 64-kD phosphoprotein in the stroma. The stromal protein phosphatase did not discriminate between dephosphorylation of phosphothreonine and phosphoserine residues in synthetic peptide substrates, providing further evidence that this enzyme is distinct from the protein phosphatase localized in thylakoid membranes. The exact physiological role of the stromal protein phosphatase has yet to be determined, but it may function in the dephosphorylation of LHC-II. 相似文献
2.
Yangyang Li Yakui Weng Xinmao Yin Xiaojiang Yu S. R. Sarath Kumar Nimer Wehbe Haijun Wu Husam N. Alshareef Stephen J. Pennycook Mark B. H. Breese Jingsheng Chen Shuai Dong Tom Wu 《Advanced functional materials》2018,28(7)
Magnetic semiconductors are highly sought in spintronics, which allow not only the control of charge carriers like in traditional electronics, but also the control of spin states. However, almost all known magnetic semiconductors are featured with bandgaps larger than 1 eV, which limits their applications in long‐wavelength regimes. In this work, the discovery of orthorhombic‐structured Ti2O3 films is reported as a unique narrow‐bandgap (≈0.1 eV) ferromagnetic oxide semiconductor. In contrast, the well‐known corundum‐structured Ti2O3 polymorph has an antiferromagnetic ground state. This comprehensive study on epitaxial Ti2O3 thin films reveals strong correlations between structure, electrical, and magnetic properties. The new orthorhombic Ti2O3 polymorph is found to be n‐type with a very high electron concentration, while the bulk‐type trigonal‐structured Ti2O3 is p‐type. More interestingly, in contrast to the antiferromagnetic ground state of trigonal bulk Ti2O3, unexpected ferromagnetism with a transition temperature well above room temperature is observed in the orthorhombic Ti2O3, which is confirmed by X‐ray magnetic circular dichroism measurements. Using first‐principles calculations, the ferromagnetism is attributed to a particular type of oxygen vacancies in the orthorhombic Ti2O3. The room‐temperature ferromagnetism observed in orthorhombic‐structured Ti2O3, demonstrates a new route toward controlling magnetism in epitaxial oxide films through selective stabilization of polymorph phases. 相似文献
3.
Noting that frequency distributions of clearness indices available in the literature are not applicable for all climates, the present article examines the suitability of a new parameter,
, and a variable,
, to depict generalized clearness index distributions.
is a normalized monthly average daily clearness index, while
is a normalized daily clearness index. These normalized clearness indices comprise maximum and minimum daily clearness indices (KT,max and KT,min), in a month, in addition to monthly average daily clearness index,
, and daily clearness index, KT. Correlations have been developed to predict KT,max and KT,min needed, in the absence of data. The distributions, developed in terms of
and
have been suitably represented and tested over 70 locations, latitudes ranging from 8°N to 64°N. The daily clearness index values have been predicted within a standard deviation of 0.034 with the expressions developed in the present study. 相似文献
4.
With the progress of human–robot interaction (HRI), the ability of a robot to perform high-level tasks in complex environments is fast becoming an essential requirement. To this end, it is desirable for a robot to understand the environment at both geometric and semantic levels. Therefore in recent years, research towards place classification has been gaining in popularity. After the era of heuristic and rule-based approaches, supervised learning algorithms have been extensively used for this purpose, showing satisfactory performance levels. However, most of those approaches have only been trained and tested in the same environments and thus impede a generalized solution. In this paper, we have proposed a semi-supervised place classification over a generalized Voronoi graph (SPCoGVG) which is a semi-supervised learning framework comprised of three techniques: support vector machine (SVM), conditional random field (CRF) and generalized Voronoi graph (GVG), in order to improve the generalizability. The inherent problem of training CRF with partially labeled data has been solved using a novel parameter estimation algorithm. The effectiveness of the proposed algorithm is validated through extensive analysis of data collected in international university environments. 相似文献
5.
K.N.V.D. Sarath Vadlamani Ravi 《Engineering Applications of Artificial Intelligence》2013,26(8):1832-1840
In this paper, we developed a binary particle swarm optimization (BPSO) based association rule miner. Our BPSO based association rule miner generates the association rules from the transactional database by formulating a combinatorial global optimization problem, without specifying the minimum support and minimum confidence unlike the a priori algorithm. Our algorithm generates the best M rules from the given database, where M is a given number. The quality of the rule is measured by a fitness function defined as the product of support and confidence. The effectiveness of our algorithm is tested on a real life bank dataset from commercial bank in India and three transactional datasets viz. books database, food items dataset and dataset of the general store taken from literature. Based on the results, we infer that our algorithm can be used as an alternative to the a priori algorithm and the FP-growth algorithm. 相似文献
6.
7.
Effect of blend ratio and elongation flow on the morphology and properties of epoxy resin‐poly(trimethylene terephthalate) blends
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C. Sarath Chandran Rene Muller M. Bouquey C. Serra S. Thomas 《Polymer Engineering and Science》2015,55(7):1679-1688
The effect of processing conditions on the morphology of polymer blends is a topic of tremendous practical interest especially for thermoset–thermoplastic blends. The effect of blend ratio and the nature of the flow field (shear flow vs. elongation flow) on the morphology is followed here using blends of diglycidyl ether of bisphenol A (DGEBA)‐poly(trimethylene terephthalate) (PTT). Morphology of the blends prepared by the conventional melt mixing technique is compared with that prepared by using an elongation mixer (RMX device invented by Muller et al.). The blends prepared by the elongation mixer showed excellent transparency and higher storage modulus at room temperature than the conventional mixer. In the case of samples prepared by the RMX device Tg of the epoxy phase has been shifted to lower temperatures indicating a molecular level mixing between PTT and DGEBA. However the conventional melt mixed samples showed only a marginal shift in Tg to low temperatures indicating that the system is not as miscible as that prepared by the RMX device. The use of RMX device for thermoset–thermoplastic blends is novel and no work has been reported in this relation. The properties of the blends were strongly affected by the composition and the crystallization of the semicrystalline PTT phase. POLYM. ENG. SCI., 55:1679–1688, 2015. © 2014 Society of Plastics Engineers 相似文献
8.
Chandrika M. Liyana‐Pathirana Fereidoon Shahidi 《Journal of the science of food and agriculture》2006,86(3):477-485
The total phenolic content (TPC), total antioxidant activity (TAA), 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) scavenging capacity, inhibition of coupled oxidation in a β‐carotene–linoleate model system, iron(II) chelation activity and inhibition of copper‐induced oxidation of human low‐density lipoprotein (hLDL) cholesterol of 80% ethanolic extracts of soft and hard winter wheat samples and their milling fractions, namely flour, germ, bran and shorts, were investigated. Soft wheat extracts examined demonstrated higher TPC and TAA compared to those of hard wheat extracts. The germ fraction possessed the highest TPC, followed by bran, shorts, whole grain and flour for both wheat types examined. The TAA of both wheat types showed similar results except that shorts performed better than bran in this assay. Free radical scavenging properties of whole grain and milling fractions of both soft and hard wheat samples were examined against DPPH radical. The germ and flour fractions demonstrated the highest and lowest DPPH radical scavenging activity, respectively, among wheat fractions. Wheat extracts were also efficient in preventing bleaching of β‐carotene, which is also known to be free radical mediated. In the iron(II) chelation assay the flour extracts demonstrated excellent activity, while the germ extracts showed a weak activity. The trends were similar in both soft and hard wheat for the iron(II) chelation assay. Wheat extracts also inhibited copper‐induced oxidation of hLDL. In LDL oxidation assay, wheat extracts performed better than the reference antioxidant, ferulic acid. Thus wheat phenolics may serve as effective antioxidative components as measured by in vitro techniques. Copyright © 2005 Society of Chemical Industry 相似文献
9.
Saltwater or brackish water is used as a coolant in most industries. Therefore, understanding the heat transfer processes and hydrodynamics during the natural convection in saline water is crucial for enhancing the efficiency of a heat exchanger. This study elaborates on the natural convection heat transfer in saline water under atmospheric conditions. A DC power supply is used to regulate the power given to the heater in a liquid pool for thermal analysis. The pool liquid comprises solutions with varying salinity from 0%, 0.2%, 0.5%, and 2%. The effect of varying salinity on the heat transfer coefficient and the thermal aspects encountered during the desalination process is analyzed. The temperature distribution across the surface of the heater is monitored using an infrared camera. It is studied for the solution of different salinities. The heat transfer coefficient and Nusselt number are investigated during natural convection for normal water and salt solution of different concentrations. It is inferred from the study that in the regime of natural convection, there is no significant difference in the Nusselt number for normal water and saltwater for the lower value of temperature difference between the plate and pool. The heat transfer coefficient in 0.2% saline water is higher as compared to the other solutions. 相似文献
10.
Rami N. Khushaba Chelsea Wise Sarath Kodagoda Jordan Louviere Barbara E. Kahn Claudia Townsend 《Expert systems with applications》2013,40(9):3803-3812
Application of neuroscience methods to analyze and understand human behavior related to markets and marketing exchange has recently gained research attention. The basic aim is to guide design and presentation of products to optimize them to be as compatible as possible with consumer preferences. This paper investigates physiological decision processes while participants undertook a choice task designed to elicit preferences for a product. The task required participants to choose their preferred crackers described by shape (square, triangle, round), flavor (wheat, dark rye, plain) and topping (salt, poppy, no topping). The two main research objectives were (1) to observe and evaluate the cortical activity of the different brain regions and the interdependencies among the Electroencephalogram (EEG) signals from these regions; and (2) unlike most research in this area that has focused mainly on liking/disliking certain products, we provide a way to quantify the importance of different cracker features that contribute to the product design based on mutual information. We used the commercial Emotiv EPOC wireless EEG headset with 14 channels to collect EEG signals from participants. We also used a Tobii-Studio eye tracker system to relate the EEG data to the specific choice options (crackers). Subjects were shown 57 choice sets; each choice set described three choice options (crackers). The patterns of cortical activity were obtained in the five principal frequency bands, Delta (0–4 Hz), Theta (3–7 Hz), Alpha (8–12 Hz), Beta (13–30 Hz), and Gamma (30–40 Hz). There was a clear phase synchronization between the left and right frontal and occipital regions indicating interhemispheric communications during the chosen task for the 18 participants. Results also indicated that there was a clear and significant change (p < 0.01) in the EEG power spectral activities taking a place mainly in the frontal (delta, alpha and beta across F3, F4, FC5 and FC6), temporal (alpha, beta, gamma across T7), and occipital (theta, alpha, and beta across O1) regions when participants indicated their preferences for their preferred crackers. Additionally, our mutual information analysis indicated that the various cracker flavors and toppings of the crackers were more important factors affecting the buying decision than the shapes of the crackers. 相似文献