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1.
A broadly cross-protective monoclonal antibody binding to Escherichia coli and Salmonella lipopolysaccharides 总被引:1,自引:0,他引:1
FE Di Padova H Brade GR Barclay IR Poxton E Liehl E Schuetze HP Kocher G Ramsay MH Schreier DB McClelland 《Canadian Metallurgical Quarterly》1993,61(9):3863-3872
During the last decade, episodes of sepsis have increased and Escherichia coli has remained the most frequent clinical isolate. Lipopolysaccharides (LPS; endotoxin) are the major toxic and antigenic components of gram-negative bacteria and qualify as targets for therapeutic interventions. Molecules that neutralize the toxic effects of LPS are actively investigated. In this paper, we describe a murine monoclonal antibody (MAb; WN1 222-5), broadly cross-reactive and cross-protective for smooth (S)-form and rough (R)-form LPS. As shown in enzyme-linked immunosorbent assay and the passive hemolysis assay, WN1 222-5 binds to the five known E. coli core chemotypes, to Salmonella core, and to S-form LPS having these core structures. In immunoblots, it is shown to react with both the nonsubstituted core LPS and with LPS carrying O-side chains, indicating the exposure of the epitope in both S-form and R-form LPS. This MAb of the immunoglobulin G2a class is not lipid A reactive but binds to E. coli J5, an RcP+ mutant which carries an inner core structure common to many members of the family Enterobacteriaceae. Phosphate groups present in the inner core contribute to the epitope but are not essential for the binding of WN1 222-5 to complete core LPS. Cross-reactivity for clinical bacterial isolates is broad. WN1 222-5 binds to all E. coli clinical isolates tested so far (79 blood isolates, 80 urinary isolates, and 21 fecal isolates) and to some Citrobacter, Enterobacter, and Klebsiella isolates. This pattern of reactivity indicates that its binding epitope is widespread among members of the Enterobacteriaceae. WN1 222-5 exhibits biologically relevant activities. In vitro, it inhibits the Limulus amoebocyte lysate assay activity of S-form and R-form LPS in a dose-dependent manner and it neutralizes the LPS-induced release of clinically relevant monokines (interleukin 6 and tumor necrosis factor). In vivo, WN1 222-5 blocks endotoxin-induced pyrogenicity in rabbits and lethality in galactosamine-sensitized mice. The discovery of WN1 222-5 settles the long-lasting controversy over the existence of anti-core LPS MAbs with both cross-reactive and cross-protective activity, opening new possibilities for the immunotherapy of sepsis caused by gram-negative bacteria. 相似文献
2.
W. Nierle W. Feldheim H. G. Maier P. Schreier W. Grosch G. -W. v. Rymon Lipinski H. -P. Thier W. Feldheim W. Seibel J. Buchberger E. Schlimme G. Engel U. Sacher F. Siewek R. Frommberger R. Frommberger G. -W. v. Rymon Lipinski F. I. Schäfers F. I. Schäfers R. Matissek H. Klostermeyer F. Kiermeier H. -P. Thier M. Miller M. Miller H. Wieczorek F. Jekat W. Feldheim K. Eichner M. Petz D. Gottschalk und W. Nierle 《Zeitschrift für Lebensmitteluntersuchung und -Forschung A》1987,185(2):134-144
3.
Souleiman I. Glumineau A. Schreier G. 《Automatic Control, IEEE Transactions on》2003,48(12):2191-2196
This paper gives necessary and sufficient conditions to solve in a constructive way the transformation of a general multiple-input-single-output nonlinear system into a state affine system. This result is direct i.e., without solving the state elimination problem when computing the input-output differential equation. A Kalman-like observer can be designed for the obtained state affine system. 相似文献
4.
Gert Schubring U. Krengel R. Tobies J. Hamel H. Remane G. Eisenreich W. Schreier 《NTM》1993,1(1):121-127
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No Abstract. 相似文献
7.
New techniques to retrieve snow specific surface area (SSA), albedo and grain size from satellite data are described. The described methods are applied to the determination of the snow properties at the coast of Greenland. It was found that the effective grain diameters were about 0.5 mm and the specific surface area was about 150 cm2g?1 for the region studied. The snow albedo was close to 0.93. The results were obtained using AATSR data. SSA spatial distribution was for the first time derived using satellite measurements. 相似文献
8.
The distribution of 15 aroma constituents, including the (R)- and (S)-enantiomers of ethyl 2-methylbutanoate, in 30 commercially available and laboratory prepared apple juices was evaluated
by multivariate statistical methods. Principal component analysis and cluster analysis revealed ethyl 3-methylbutanoate, 3-methylbutyl
acetate and (R)-ethyl 2-methylbutanoate as key components for the differentiation of apple juices. In freshly pressed and some commercial
juices ethyl 3-methylbutanoate and 3-methylbutyl acetate were not present, and the (S):(R) ratio of ethyl 2-methylbutanoate was found to be >99 : 1%. Another group of commercial products was separated in which the
amounts of ethyl 3-methylbutanoate and 3-methylbutyl acetate exceeded 1 μg/l and 180 μg/l, respectively, and in which the
(S):(R) ratio of ethyl 2-methylbutanoate was <97 : 3%. The statistical data confirmed the high selectivity of apple fruit tissue
in biosynthesizing 2-methylbutyl and 2-methylbutanoate esters, and could also be used to extend the results of enantio-multidimensional
gas chromatography and other authenticity analyses.
Received: 20 April 1998 / Revised version: 23 June 1998 相似文献
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