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排序方式: 共有208条查询结果,搜索用时 15 毫秒
1.
Priyadarshi Chakraborty Yiming Tang Tomoya Yamamoto Yifei Yao Tom Guterman Shai Zilberzwige-Tal Nofar Adadi Wei Ji Tal Dvir Ayyalusamy Ramamoorthy Guanghong Wei Ehud Gazit 《Advanced materials (Deerfield Beach, Fla.)》2020,32(9):1906043
Self-assembled peptide hydrogels represent the realization of peptide nanotechnology into biomedical products. There is a continuous quest to identify the simplest building blocks and optimize their critical gelation concentration (CGC). Herein, a minimalistic, de novo dipeptide, Fmoc-Lys(Fmoc)-Asp, as an hydrogelator with the lowest CGC ever reported, almost fourfold lower as compared to that of a large hexadecapeptide previously described, is reported. The dipeptide self-assembles through an unusual and unprecedented two-step process as elucidated by solid-state NMR and molecular dynamics simulation. The hydrogel is cytocompatible and supports 2D/3D cell growth. Conductive composite gels composed of Fmoc-Lys(Fmoc)-Asp and a conductive polymer exhibit excellent DNA binding. Fmoc-Lys(Fmoc)-Asp exhibits the lowest CGC and highest mechanical properties when compared to a library of dipeptide analogues, thus validating the uniqueness of the molecular design which confers useful properties for various potential applications. 相似文献
2.
A group of people taking pictures of a dynamic event with their mobile phones is a popular sight. The set of still images obtained this way is rich in dynamic content but lacks accurate temporal information. We propose a method for photo-sequencing—temporally ordering a set of still images taken asynchronously by a set of uncalibrated cameras. Photo-sequencing is an essential tool in analyzing (or visualizing) a dynamic scene captured by still images. The first step of the method detects sets of corresponding static and dynamic feature points across images. The static features are used to determine the epipolar geometry between pairs of images, and each dynamic feature votes for the temporal order of the images in which it appears. The partial orders provided by the dynamic features are not necessarily consistent, and we use rank aggregation to combine them into a globally consistent temporal order of images. We demonstrate successful photo-sequencing on several challenging collections of images taken using a number of mobile phones. 相似文献
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Automated regression suites are essential in developing large applications, while maintaining reasonable quality and timetables. The main argument against the automation of regression suites, in addition to the cost of creation and maintenance, is the observation that if you run the same test many times, it becomes increasingly less likely to find bugs. To alleviate such problems, a new regression suite practice, using random test generators to create regression suites on-the-fly, is becoming more common. In this practice, instead of maintaining tests, we generate test suites on-the-fly by choosing several specifications and generating a number of tests from each specification. 相似文献
5.
Dorit Baras Shai Fine Laurent Fournier Dan Geiger Avi Ziv 《International Journal on Software Tools for Technology Transfer (STTT)》2011,13(3):247-261
Closing the feedback loop from coverage data to the stimuli generator is one of the main challenges in the verification process.
Typically, verification engineers with deep domain knowledge manually prepare a set of stimuli generation directives for that
purpose. Bayesian networks based CDG (coverage directed generation) systems have been successfully used to assist the process
by automatically closing this feedback loop. However, constructing these CDG systems requires manual effort and a certain
amount of domain knowledge from a machine learning specialist. We propose a new method that boosts coverage in the early stages
of the verification process with minimal effort, namely a fully automatic construction of a CDG system that requires no domain
knowledge. Experimental results on a real-life cross-product coverage model demonstrate the efficiency of the proposed method. 相似文献
6.
Netanel Aharon Shai Machnes Benni Reznik Jonathan Silman Lev Vaidman 《Natural computing》2013,12(1):5-8
We present a family of nonlocal games in which the inputs the players receive are continuous. We study three representative members of the family. For the first two a team sharing quantum correlations (entanglement) has an advantage over any team restricted to classical correlations. We conjecture that this is true for the third member of the family as well. 相似文献
7.
Crosslinked poly(ester urethane)s and their acrylate derivatives based on trifunctional polycaprolactone and trifunctional aliphatic isocyanates were synthesized. Biodegradable scaffolds with uniform, controlled micron-scale porosity were fabricated with these materials. Mechanical and swelling properties of monolithic and microporous materials were studied. Cytotoxicity, hydrolytic, and enzymatic degradation and their effects on mechanical properties of the biodegradable scaffolds were investigated. The polymer degradation products were found not to be cytotoxic at moderate concentrations and to permit cell attachment and spreading. Degradation rates and mechanical properties could be tuned to desired performance criteria for a given application by adjusting crosslink density and the ratio of hard segment to soft segment. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2020 , 137, 48943. 相似文献
8.
Contamination of poultry by Campylobacter spp. is a significant source of human diarrheal diseases. Traditional methods currently used to detect Campylobacter in foods are time-consuming and labor-intensive. In this study, primers designed for the Campylobacter jejuni cadF gene sequence were used in a SYBR Green I real-time PCR assay as an alternative to a conventional bacteriological method for the rapid detection of C. jejuni from poultry. Twelve portions of chicken purchased from two local grocery stores and 39 portions obtained from a commercial processing plant were examined. Samples of the skin were enriched in Bolton broth at 37 degrees C for 3 h and then at 42 degrees C for 9, 21, or 45 h under microaerobic conditions. DNA was extracted from 1-ml aliquots of the enrichment cultures using 1% Triton X-100. The DNA was used as the template in a real-time polymerase chain reaction (PCR) assay. After 24 h of enrichment, C. jejuni was isolated from 13 samples and all of the positive cultures were also detected by the real-time PCR procedure. C. jejuni was detected by both methods from samples artificially contaminated with 1 or 10 CFU of C. jejuni per 10 g, after 24 h of enrichment. The real-time PCR method was found to be sensitive and specific. It significantly reduced the time required for the detection of C. jejuni in poultry following enrichment of samples. 相似文献
9.
Primers designed to amplify a Campylobacter jejuni cadF gene sequence were used in an SYBR Green I real-time PCR assay as an alternative to conventional bacteriological methods for the rapid detection of C. jejuni in foods. Twenty-five grams of chicken skin (breast and thigh) was contaminated by adding approximately 1, 10, or 50 CFU of C. jejuni ATCC 35560. Twenty-five grams of pork and 25-ml aliquots of milk were also inoculated with 1 and 10 CFU of the pathogen. The samples were incubated in Bolton broth for different periods at 37 and 42 degrees C under microaerophilic conditions. Using a commercial robotic DNA purification system, DNA was extracted and purified from 1-ml aliquots of the enrichment cultures before and after centrifugation of the 250-ml enrichment broth at 15,900 x g for 10 min at 4 degrees C. The DNA was used as the template in a real-time PCR assay. C. jejuni was detected after 12 h of enrichment from samples inoculated with about 50 CFU/25-g sample. After centrifugation, an enrichment step of 8 h was sufficient to allow detection of pathogen in samples inoculated with 10 CFU/25 g. However, 24 h of enrichment was necessary to detect pathogen in samples inoculated with approximately 1 CFU/25 g. The real-time PCR protocol developed in this study significantly reduced the detection time of C. jejuni in foods. 相似文献
10.
目的总结覆膜支架腔内修复术治疗Standford B型主动脉夹层的经验,分析围手术期并发症的发生率及处理方法。方法2005年1月至2010年1月,127例B型主动脉夹层患者接受覆膜支架腔内修复术治疗。B型夹层112例,B型主动脉穿透性溃疡15例。术前、术后10d行64排CTA检查,证实手术效果。统计分析围手术期病死率、主要并发症发生率,探讨死亡及发生主要并发症的可能原因。结果手术成功率100%,围手术期死亡3例,病死率为2.4%,主要并发症发生率为13.4%(17/127),包括截瘫1例,逆行性A型夹层1例,脑梗死致意识障碍2例,支架相关并发症9例,严重内漏4例。结论覆膜支架腔内修复术治疗B型主动脉夹层安全、有效,但主要并发症的发生不容忽视。 相似文献