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1.
Intraseptal scopolamine has differential effects on Pavlovian eye blink and heart rate conditioning.
Evaluated the effects of intraseptal scopolamine hydrobromide (40 μg) injections on Pavlovian (classical) conditioning, with tones used as the CS and a periorbital electric shock train as the UCS, using New Zealand albino rabbits. Eyeblink (EB) and heart rate (HTR) CRs were concomitantly recorded. Although injections of scopolamine into the medial septum impaired the acquisition of the Pavlovian conditioned eyelid reflex, these injections enhanced the magnitude of accompanying Pavlovian conditioned HTR decelerations. However, scopolamine applied to the lateral septal area had no effect on EB conditioning, relative to the vehicle; like medial injections, scopolamine also enhanced the magnitude of the accompanying HTR decelerations. Results are compatible with those of previous investigations indicating that medial septal dysfunction impairs somatomotor conditioning but leaves autonomic conditioning intact and that septal dysfunction produces a parasympathetic bias of the cardiovascular system. (49 ref) (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
2.
Geoffrey Rowden Shirley Dean James Campbell Philip Belitsky 《Microscopy research and technique》1985,2(6):547-555
Aspiration biopsy of tumors and transplanted organs is a popular atraumatic method for diagnosis and also for the management of patients. Apart from the cytological analyses, it is clear that large aggregates of cells are obtained and these may be examined by electron microscopy. A study was carried out with dogs to assess the yield of intact glomeruli and tubules, resulting from a standard fine-needle aspiration procedure. Employing a 20-gauge needle, yields of approximately ten intact glomeruli were obtained with a tissue processing protocol that utilized filtration through a nylon mesh and backwashing, to resuspend the trapped aggregates. Processing for electron microscopy was carried out in microcentrifuge tubes. The ultrastructural preservation was excellent and the extension of these studies to investigations of not only renal transplantation, but also for diagnosis in human renal diseases of a broad spectrum, is suggested. 相似文献
3.
Kerlavage A.R. FitzHugh W. Gladek A. Kelley J. Scott J. Shirley R. Sutton G. Man Wai-Chiu White O. Adams D. 《IEEE engineering in medicine and biology magazine》1995,14(6):710-717
The rapid advances in molecular biology have begun to shift many of the bottlenecks in genome research from the laboratory to the data analysis facility. The pace at which this has occurred creates a situation in which software development always has to catch up with the flow of data. Since such large-scale processes were not anticipated, the analysis infrastructure has not been fully established. Furthermore, most systems that have been built were designed by the biologists who collected the data. More recently, computer scientists, mathematicians, and engineers have taken an interest in this problem. This has had a positive effect, since it has created a tight synergy between the informatics and the biology. Several principles affected the design of the system developed at TIGR. Each of the sample preparation, sequencing, and analysis steps had to be managed, scheduled, and tracked. This information had to be made readily available to those who needed it for carrying out their tasks. Different skill levels of the users had to be taken into account. The degree of human intervention at each step had to be evaluated and built into the design. A mixed processing environment of Macintosh and Unix platforms had to be integrated. Most importantly, the system had to save time, reduce error, and ensure uniformity of the analysis and quality of the results. In the authors' experience, the tools they have built work well because of their early decisions as to which systems to use for development. The authors settled on a robust relational database management system (Sybase) and a portable development environment (C, C++) 相似文献
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我在德国学者与摄影几乎毫不相关的专业——计算机科学,成天打交道的,除了数字就是计算机,家里几乎都是学理工科出身的。小的时候,我虽然也曾在闲暇时学习间乐绘画,算是与艺术沾上点边,自己也是很感兴趣,但真到选择专业的时候,却被告知,这碗饭并不好吃,生活也就还是被拉回“正常”的轨道。对艺术,对设计的好感也就因为找不到宣泄的地方暂时搁置下来,摄影,在那个时候还没有在我的生活中出现过。 相似文献
6.
Alexander Zimmermann Qais Z. Jaber Dr. Johannes Koch Steffen Riebe Dr. Cecilia Vallet Dr. Kateryna Loza Matthias Hayduk Dr. Kfir B. Steinbuch Prof. Shirley K. Knauer Prof. Micha Fridman Jun.-Prof. Jens Voskuhl 《Chembiochem : a European journal of chemical biology》2021,22(9):1563-1567
We report the characterization of amphiphilic aminoglycoside conjugates containing luminophores with aggregation-induced emission properties as transfection reagents. These inherently luminescent transfection vectors are capable of binding plasmid DNA through electrostatic interactions; this binding results in an emission “on” signal due to restriction of intramolecular motion of the luminophore core. The luminescent cationic amphiphiles effectively transferred plasmid DNA into mammalian cells (HeLa, HEK 293T), as proven by expression of a red fluorescent protein marker. The morphologies of the aggregates were investigated by microscopy as well as ζ-potential and dynamic light-scattering measurements. The transfection efficiencies using luminescent cationic amphiphiles were similar to that of the gold-standard transfection reagent Lipofectamine® 2000. 相似文献
7.
Tensile properties of polyethertherketone (PEEK) have been studied at 125, 25, and ?100°C for thin films prepared with different thermal histories. Initial morphology was controlled by rate of cooling from the melt. Amorphous films resulted from quenching the melt, while semicrystalline films were obtained by cooling the melt at different rates, or by crystallization of the rubbery amorphous state. The films were characterized using density, X-ray scattering, differential scanning calorimetry, and infrared spectroscopy. Scanning electron microscopy was used to examine fracture surfaces. Degree of crystallinity and rate of cooling from the melt affected the tensile properties at all test temperatures. For films with nearly the same degree of crystallinity, those which were more slowly cooled from the melt fractured at the lowest strain. The amorphous films were most tough, drawing to 233% at ?100°C and to over 500% at 125°C. Films crystallized from the rubbery amorphous state had stress–strain behavior intermediate between that of the amorphous and melt-crystallized films at all test temperatures. Density measurements on the drawn material indicate that void formation occurs simultaneously with the formation of fibrillar crystals. Necking resulted in density increases for amorphous films, and density decreases for the semicrystalline films. 相似文献
8.
Understanding urban residential end uses of water 总被引:1,自引:0,他引:1
The 'end use' of water is a breakdown of the total household water usage such as water used for toilets, showers, washing machines, taps, lawn watering, etc. Understanding end uses of water will enable water planners, water authorities and household owners determine where water is used/wasted, how much and how often. This paper describes the end uses of water from a number of single-family homes in Greater Melbourne, Australia. The study involves the analysis of water consumption data recorded at 5-s intervals from logged households collected by Yarra Valley Water in Melbourne in 2004. The study determines how much water is used for outdoor and indoor purposes in a single-family home in Melbourne and compares the water usage during winter and summer. Hourly patterns of major end uses of water are also developed. The aim of this study is to improve the understanding of the end uses of water and to assist where to focus water conservation efforts that would be most effective financially and environmentally, and be acceptable to everyone. 相似文献
9.
10.
Wyman C Parker S Shirley P Hansen C 《IEEE transactions on visualization and computer graphics》2006,12(2):186-196
In many applications, volumetric data sets are examined by displaying isosurfaces, surfaces where the data, or some function of the data, takes on a given value. Interactive applications typically use local lighting models to render such surfaces. This work introduces a method to precompute or lazily compute global illumination to improve interactive isosurface renderings. The precompiled illumination resides in a separate volume and includes direct light, shadows, and intersections. Using this volume, interactive globally illuminated renderings of isosurfaces become feasible while still allowing dynamic manipulation of lighting, viewpoint and isovalue. 相似文献